Enzymology

Mechanisms of enzyme catalysis are important to many researchers in the area of biochemistry. The most common methods of enzyme activity measurement involve monitoring absorbance or fluorescence of a solution of enzyme and substrate as a function of time. The change in absorbance or fluorescence reports the increase in product concentration as the reaction proceeds.

Steady-State Kinetics

Steady-state kinetics measurements involve monitoring the absorbance or fluorescence change exhibited by an enzyme/substrate complex at constant concentration throughout the measurement. Several Olis instruments support the measurement of steady-state kinetics. These include such absorbance models as the HPDA 8452, Cary 14, Cary 17, DW2, DW2000, DB620, and RSM 1000, as well as fluorescence models such as the DM 45 and DM 245.

Pre-steady State Kinetics

Pre-steady state kinetics involve measuring of the formation of the enzyme/substrate complex. A stopped flow accessory can be added to the majority of these instruments. Other accessories include the TLC 50, Four cell Peltier turrets, and the Automated Enzyme Assay Device. The Olis CLARiTY chamber offers an exciting new possibility of measuring enzyme kinetics in highly scattering environments such as those in whole cell or mitochondria suspensions.

I Efimov, CN Cronin, WS McIntire. (2001). Effects of Noncovalent and Covalent FAD Binding on the Redox and Catalytic Properties of p-Cresol Methylhydroxylase.
      Biochemistry 40, 2155-2166
Achieved using the OLIS RSM 1000 SF

S Nisar and SA Kazmi. (2011). Reductive release of Fe(III) from Bovine Lactoferrin by Ascorbic Acid.
      J. Chemical Society of Pakistan 33, 55-64
Achieved using the OLIS HPDA 8452

FP Guengerich, WA McCormick, JB Wheeler. (2003). Analysis of the Kinetic Mechanism of Haloalkane Conjugation by Mammalian ?-Class Glutathione Transferases.
      Chem. Res. Toxicol. 16, 1493-1499
Achieved using the OLIS RSM 1000/Cary 14

JL Roose, Y Kashino, HB Pakrasi. (2007). The PsbQ protein defines cyanobacterial Photosystem II complexes with highest activity and stability .
      PNAS 104, 2548-2553
Achieved using the OLIS DW2000

R Covian and BL Trumpower. (2009). Ilicicolin Inhibition and Binding at Center N of the Dimeric Cytochrome bc1 Complex Reveal Electron Transfer and Regulatory Interactions between Monomers .
      J Biol Chem 284, 8614-8620
Achieved using the OLIS RSM 1000

CS Barker, IV Meshcheryakova, T Sasaki, MC Roy, PK Sinha, T Yagi, FA Samatey. (2014). Randomly selected suppressor mutations in genes for NADH:quinone oxidoreductase-1, which rescue motility of a?Salmonella?ubiquinone-biosynthesis mutant strain.
      Microiology 160, 1075-1086
Achieved using the OLIS DW2000

S Raboni, A Mozzarelli, PF Cook. (2007). Control of Ionizable Residues in the Catalytic Mechanism of Tryptophan Synthase from Salmonella typhimurium.
      Biochemistry 46, 13223-13234
Achieved using the OLIS RSM 1000

L Yi and SW Ragsdale. (2007). Evidence That the Heme Regulatory Motifs in Heme Oxygenase-2 Serve as a Thiol/Disulfide Redox Switch Regulating Heme Binding .
      J Biol Chem 282, 21056-21067
Achieved using the OLIS Cary 14

R Saha, AT Verseput, BM Berla, TJ Mueller, HB Pakarasi, CD Maranas. (2012). Reconstruction and Comparison of the Metabolic Potential of Cyanobacteria?Cyanothece?sp. ATCC 51142 and?Synechocystissp. PCC 6803.
      PLoS ONE 7(10): e48285
Achieved using the OLIS DW 2000

BC hill, L Becker, V Anand, A Kusmierczyk, SM Marcovina, MN Rahman, BR Gabel, Z Jia, MB Boffa, ML Koschinsky. (2003). A role for apolipoprotein(a) in protection of the low-density lipoprotein component of lipoprotein(a) from copper-mediated oxidation.
      ABB 412, 186-195
Achieved using the OLIS RSM 1000

EM Isin, CD Sohl, RL Eoff, FP Guengerich. (2008). Cooperativity of cytochrome P450 1A2: Interactions of 1,4-phenylene diisocyanide and 1-isopropoxy-4-nitrobenzene .
      ABB 473, 69-75
Achieved using the OLIS Cary 14/DW2/DM 45/RSM 1000

O Guryev, RA Carvalho, S Usanov, A Gilep, RW Estabrook. (2003). A pathway for the metabolism of vitamin D3: Unique hydroxylated metabolites formed during catalysis with cytochrome P450scc (CYP11A1) .
      PNAS 100, 14754-14759
Achieved using the OLIS DW2

RK Nandigama and DL Edmondson. (2000). Structure?Activity Relations in the Oxidation of Phenethylamine Analogues by Recombinant Human Liver Monoamine Oxidase A.
      Biochemistry 39, 15258-15265
Achieved using the OLIS RSM 1000 SF

I Smirnova, V Kasho, HR Kaback. (2008). Protonation and sugar binding to LacY.
      PNAS 105, 8896-8901
Achieved using the OLIS SLM 8100

R Covian and BL Trumpower. (2006). Regulatory Interactions between Ubiquinol Oxidation and Ubiquinone Reduction Sites in the Dimeric Cytochrome bc1 Complex .
      J Biol Chem 281, 30925-30932
Achieved using the OLIS RSM 1000

MM Whittaker and JW Whittaker. (2003). Cu(I)-dependent Biogenesis of the Galactose Oxidase Redox Cofactor .
      J Biol Chem 278, 22090-22101
Achieved using the OLIS RSM 1000

N Purwar, JM McGarry, J Kostera, AA Pacheco, M Schmidt. (2011). The Interaction of Nitric Oxide With Catalase: Structural, and Kinetic Analysis.
      Biochemistry 51, 4491-4503
Achieved using the OLIS RSM 1000

BK Barr and RJ Holewinski. (2002). 4-Methyl-7-thioumbelliferyl-?-d-cellobioside:? A Fluorescent, Nonhydrolyzable Substrate Analogue for Cellulases.
      Biochemistry 41, 4447-4452
Achieved using the OLIS SLM 8000

RL Walsky, RS Obach, R Hyland, P Kang, S Zhou, M West, KF Geoghegan, CJ Helal, GS Walker, TG Goosen, MA Zientek. (2012). Selective Mechanism-Based Inactivation of Cytochrome P4503a4 by Cyp3cide (Pf-04981517) and its Utility as an In Vitro Tool for Delineating the Relative Roles of Cytochrome P4503a4 Versus P4503a5 in the Metabolism of Drugs.
      Drug Metabolism and Disposition 40, 1686-1697
Achieved using the OLIS DW2000

C Feng, RV Kedia, JT Hazzard, JK Hurley, G Tollin, JH Enemark. (2002). Effect of Solution Viscosity on Intramolecular Electron Transfer in Sulfite Oxidase.
      Biochemistry 41, 5816-5821
Achieved using the OLIS SIFIT

M Martinez-Julvez, I Nogues, M Faro, JK Hurley, TB Brodie, T Mayoral, J Sanz-Aparicio, JA Hermoso, MT Stankovich, M Medina, G Tollin, C Gomez-Moreno. (2001). Role of a Cluster of Hydrophobic Residues Near the FAD Cofactor in Anabaena PCC 7119 Ferredoxin-NADP+Reductase for Optimal Complex Formation and Electron Transfer to Ferredoxin .
      J Biol Chem 276, 27498-27510
Achieved using the OLIS Cary 15

LV Lee, B Granda, K Dean, J Tao, E Lui, R Zhang, S Peukert, S Wattanasin, X Xie, NS Ryder, R Tommasi, G Deng. (2010). Biophysical Investigation of the Mode of Inhibition of Tetramic Acids, the Allosteric Inhibitors of Undecaprenyl Pyrophosphate Synthase.
      Biochemistry 49, 5366-5376
Achieved using the OLIS RSM 1000F

WM Rabeh, SS Alguindigue, PF Cook. (2005). Mechanism of the Addition Half of the O-Acetylserine Sulfhydrylase-A Reaction.
      Biochemistry 44, 5541-5550
Achieved using the OLIS RSM 1000 SF

RS Phillips, S Lima, R Khristoforov, B Sodararaju. (2010). Insights into the Mechanism of Pseudomonas dacunhae Aspartate ?-Decarboxylase from Rapid-Scanning Stopped-Flow Kinetics.
      Biochemistry 49, 5066-5073
Achieved using the OLIS RSM 1000

M Choi, N Sukumar, FS Matthews, A Liu, VL Davidson. (2010). Proline96 of the copper ligand loop of amicyanin regulates electron transfer from methylamine dehydrogenase by positioning other residues at the protein-protein interface.
      Biochemistry 50, 1265-1273
Achieved using the OLIS RSM 1000

AS Fernandes, AA Konstantinov, M Teixeira, MM Pereira. (2005). Quinone reduction by?Rhodothermus marinus?succinate:menaquinone oxidoreductase is not stimulated by the membrane potential.
      BBRC 330, 565-570
Achieved using the OLIS DW2

D Jancura, J Stanicova, G Palmer, M Fabian. (2014). How Hydrogen Peroxide Is Metabolized by Oxidized Cytochrome c Oxidase.
      Biochemistry 53, 3564-3575
Achieved using the OLIS RSM 1000

S Herold, M Exner, T Nauser. (2001). Kinetic and Mechanistic Studies of the NO-mediated Oxidation of Oxymyoglobin and Oxyhemoglobin.
      Biochemistry 40, 3385-3395
Achieved using the OLIS RSM 1000

K Stark, M Dostalek, FP Guengerich. (2008). Expression and Purification of Orphan Cytochrome P450 4X1 and Oxidation of Anandamide.
      FEBS 275, 3706-3717
Achieved using the OLIS DW2

JN Lampe, C Fernandez, A Nath, WM Atkins. (2008). Nile Red Is a Fluorescent Allosteric Substrate of Cytochrome P450 3A4.
      Biochemistry 47, 509-516
Achieved using the OLIS DW2

WE Karsten and PF Cook. (2009). Detection of a gem-diamine and a stable quinonoid intermediate in the reaction catalyzed by serine?glyoxylate aminotransferase from Hyphomicrobium methylovorum?.
      BBA 1790, 575
Achieved using the OLIS RSM 1000

RS Phillips and G Holterman. (2005). Differential Effects of Temperature and Hydrostatic Pressure on the Formation of Quinonoid Intermediates from l-Trp and l-Met by H463F Mutant Escherichia coli Tryptophan Indole-lyase.
      Biochemistry 44, 14289-14297
Achieved using the OLIS RSM/Cary 14

Gchowdhury, MW Calcutt, LD Nagy, FP Guengerich. (2012). Oxidation of Methyl and Ethyl Nitrosamines by Cytochromes P450 2E1 and 2B1.
      Biochemistry 51, 9995-10007
Achieved using the OLIS HPDA 8452

S Shin, NA Tarboush, and VL Davidson. (2010). Long-Range Electron Transfer Reactions between Hemes of MauG and Different Forms of Tryptophan Tryptophylquinone of Methylamine Dehydrogenase.
      Biochemistry 49, 5810-5816
Achieved using the OLIS RSM 1000

C-A Yu, X Wen, K Xiao, D Xia, L Yu. (2002). Inter- and intra-molecular electron transfer in the cytochrome?bc1?complex.
      BBA Bioenergetics 1555, 65-70
Achieved using the OLIS RSM 1000

D Sun, K Ono, T Okajima, K Tanizawa, M Uchida, Y Yamamoto, FS Matthews, VL Davidson. (2003). Chemical and Kinetic Reaction Mechanisms of Quinohemoprotein Amine Dehydrogenase from Paracoccus denitrificans.
      Biochemistry 42, 10896-10903
Achieved using the OLIS RSM 1000

S Gomez-Manzo, JL Chavez-Pacheco, M Contreras-Zentella, ME SosaTorres, R Arreguin-Espinosa, M Perez de la Mora, J Membrillo-Hernandez, JE Escamilla. (2010). Molecular and Catalytic Properties of the Aldehyde Dehydrogenase of?Gluconacetobacter diazotrophicus, a Quinoheme Protein Containing Pyrroloquinoline Quinone, Cytochrome?b, and Cytochrome?c.
      J Bacteriology 192, 5718-5724
Achieved using the OLIS DW2000

GA Hunter, J Zhang, GC Ferreira. (2007). Transient Kinetic Studies Support Refinements to the Chemical and Kinetic Mechanisms of Aminolevulinate Synthase .
      J Biol Chem 282, 23025-23035
Achieved using the OLIS RSM 1000F

WA Alves, SA de Almeida-Filho, MV de Almeida, A Paduan-Filho, CC Becerra, AM DaCosta Ferreira. (2003). Comparative kinetic studies on tyrosinase-like catalytic activity of dinuclear imidazole-containing copper(II) complexes.
      J Mol Catalysis A: Chemical 198, 63-75
Achieved using the OLIS DW 2000

B Zhao, FP Guengerich, M Voehler, MR Waterman. (2005). Role of Active Site Water Molecules and Substrate Hydroxyl Groups in Oxygen Activation by Cytochrome P450 158A2.
      J. Biological Chemistry 280, 42188-42197
Achieved using the OLIS Cary 14

DA Mills, S xu, L Gereu, C Hiser, L Qin, MA Sharpe, J McCracken, B Durham, F Millett, S Ferguson-Miller. (2008). Proton-Dependent Electron Transfer from CuA to Heme a and Altered EPR Spectra in Mutants Close to Heme a of Cytochrome Oxidase.
      Biochemistry 47, 11499-11509
Achieved using the OLIS RSM 1000 SF

HJ Nothnagel, MR Preimesberger, MP Pond, BY Winer, EM Adney, JTJ Leconte. (2011). Chemical reactivity of?Synechococcus?sp. PCC 7002 and?Synechocystis?sp. PCC 6803 hemoglobins: covalent heme attachment and bishistidine coordination.
      J Biological Inorganic Chemistry 16, 539-552
Achieved using the OLIS RSM 1000

A Melet, N Assrir, P Jean, MP Lopez-Garcia, C Marques-Soares, M Jaouen, PM Dansette, M-A sari, D Mansury. (2003). Substrate selectivity of human cytochrome P450 2C9: importance of residues 476, 365, and 114 in recognition of diclofenac and sulfaphenazole and in mechanism-based inactivation by tienilic acid.
      ABB 409, 80-91
Achieved using the OLIS DW2

Y Zhao, DA Lukovanov, YV Toropov, K Wu, JP Shapleigh, CP Scholes. (2002). Catalytic Function and Local Proton Structure at the Type 2 Copper of Nitrite Reductase:? The Correlation of Enzymatic pH Dependence, Conserved Residues, and Proton Hyperfine Structure.
      Biochemistry 41, 7464-7474
Achieved using the OLIS DM 45 SF

NT Ha-Duong, S Dijols, AC Macherey, JA Goldstein, PM Dansette, D Mansuy. (2001). Ticlopidine as a Selective Mechanism-Based Inhibitor of Human Cytochrome P4502C19.
      Biochemistry 40, 12112-12122
Achieved using the OLIS DW-2

S Herold and F-JK Rehmann. (2003). Kinetics of the reactions of nitrogen monoxide and nitrite with ferryl hemoglobin.
      Free Radical Biology and Medicine 34, 531-545
Achieved using the OLIS RSM 1000

TE Swartz, I Szundi, JL Spudich, RA Bogomolni. (2000). New Photointermediates in the Two Photon Signaling Pathway of Sensory Rhodopsin-1.
      Biochemistry 39, 15101-15109
Achieved using the OLIS DW-2

LK Patterson, J-C Maziere, DM Bartels, GL Hug, R Santus, P Morliere. (2010). Evidence for a slow and oxygen-insensitive intra-molecular long range electron transfer from tyrosine residues to the semi-oxidized tryptophan 214 in human serum albumin: its inhibition by bound copper (II).
      Amino Acids 42, 1269-1275
Achieved using the OLIS RSM 1000

S Shin, S Lee, VL Davidson. (2009). Suicide Inactivation of MauG during Reaction with O2 or H2O2 in the Absence of Its Natural Protein Substrate.
      Biochemistry 48, 10106-10112
Achieved using the OLIS RSM 1000

RLS Goncalves, DE Rothschild, CL Quinlan, GK Scott, CC Benz, MD Brand. (2014). Sources of superoxide/H2O2?during mitochondrial proline oxidation.
      Redox Biology 2, 901-909
Achieved using the OLIS DW 2

CD Sohl, EM Isin, RL Eoff, GA Marsch, DF Stec, FP Guengerich. (2008). Cooperativity in Oxidation Reactions Catalyzed by Cytochrome P450 1A2: Highly Coorerative Pyrene Hydroxylation and Multiphasic Kinetics and Ligand Binding.
      J. Biological Chemistry 283, 7293-7308
Achieved using the OLIS HPDA

R Covian and BL Trumpower. (2005). Rapid Electron Transfer between Monomers when the Cytochrome bc1 Complex Dimer Is Reduced through Center N .
      J Biol Chem 280, 22732-22740
Achieved using the OLIS RSM 1000

E Ritter, K Zimmermann, M Heck, KP Hofmann, FJ Bartl. (2004). Transition of Rhodopsin into the Active Metarhodopsin II State Opens a New Light-induced Pathway Linked to Schiff Base Isomerization .
      J Biol Chem 279, 48102-48111
Achieved using the OLIS RSM 1000

M Gutierrez-Aguilar, X Perez-Martinez, E Chavez, S Uribe-Carajal. (2010). In Saccharomyces cerevisiae, the phosphate carrier is a component of the mitochondrial unselective channel.
      ABB 494, 184-191
Achieved using the OLIS DW2

A Sreedhara, JD Freed, JA Cowan. (2000). Efficient Inorganic Deoxyribonucleases. Greater than 50-Million-fold Rate Enhancement in Enzyme-Like DNA Cleavage.
      JACS 122, 8814-8824
Achieved using the OLIS HPDA 8452

J Kauny and P Setif. (2014). NADPH fluorescence in the cyanobacterium Synechocystis sp. PCC 6803: A versatile probe for in vivo measurements of rates, yields and pools.
      Biochimical Biophysica Acta: Bioenergetics 1837, 792-801
Achieved using the OLIS DW 2000

MA Khan, H Miyoshi, S Ray, T Natsuaki, N Suehiro, DJ Goss. (2006). Interaction of Genome-linked Protein (VPg) of Turnip Mosaic Virus with Wheat Germ Translation Initiation Factors eIFiso4E and eIFiso4F .
      J Biol Chem 281, 28002-28010
Achieved using the OLIS RSM 1000F

GJ Colpas and RP Hausinger. (2000). In Vivo and in Vitro Kinetics of Metal Transfer by the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE*,210.
      J Biol chem 275, 10731-10737
Achieved using the OLIS RSM 1000

EA Cowa, CD Oldham, SW May. (2010). Identification of a thioselenurane intermediate in the reaction between phenylaminoalkyl selenoxides and glutathione.
      Arch. Biochem. Biophys 506, 201-207
Achieved using the OLIS RSM 1000

JJ Jung, AR Choi, YK Lee, HK Lee, K-H Jung. (2008). Spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the Arctic Ocean .
      FEBS Lett. 582, 1679-1684
Achieved using the OLIS RSM 1000

B Gonzalez, S Martinez, JL Chavez, S Lee, NA Castro, MA Dominguez, S Gomez, ML Contreras, C Kennedy, JE Escamilla. (2006). Respiratory system of?Gluconacetobacter diazotrophicus?PAL5 Evidence for a cyanide-sensitive cytochrome?bb?and cyanide-resistant cytochrome?ba?quinol oxidases.
      BBA Bioenergetics 1757, 1614-1622
Achieved using the OLIS DW 2000

MA Hummel, PM Gannett, J Aguilar, TS Tracy. (2008). Substrate proton to heme distances in CYP2C9 allelic variants and alterations by the heterotropic activator, dapsone.
      ABB 475, 175-183
Achieved using the OLIS DW2000

YJ Chun, T Shimada, R Sanchez-Ponce, MV Martin, L Lei, B Zhao, SL Kelly, MR Waterman, DC Lamb, FP Guengerich. (2007). Electron Transport Pathway for a Streptomyces Cytochrome P450: Cytochrome P450 105D5 - Catalyzed Fatty Acid Hydrolysis in Streptomyces Coelicolor A3(2).
      J. Biological Chemistry 282, 17486-17500
Achieved using the OLIS RSM 1000 SF

AL Blobaum. DL Harris, PF Hollenberg. (2005). P450 Active Site Architecture and Reversibility: Inactivation of Cytochromes P450 2B4 and 2B4 T302A by tert-Butyl Acetylenes.
      Biochemistry 44, 3831-3844
Achieved using the OLIS DW-2

G Chowdhury, MW Calcutt, FP Guengerich. (2010). Oxidation of N-Nitrosoalkylamines by Human Cytochrome P450 2A6: SEQUENTIAL OXIDATION TO ALDEHYDES AND CARBOXYLIC ACIDS AND ANALYSIS OF REACTION STEPS .
      JBC 285, 8031-8044
Achieved using the OLIS RSM 1000/DW2

X Perez-Martinez, M Vazquez-Acevedo, E Tolkunova, S Funes, MG Claros, E Davidson, MP King, D Gonzalez-Halphen. (2000). Unusual Location of a Mitochondrial Gene: SUBUNIT III OF CYTOCHROME c OXIDASE IS ENCODED IN THE NUCLEUS OF CHLAMYDOMONAD ALGAE .
      J Biol Chem 275, 30144-30152
Achieved using the OLIS DW2

EM Isin and FP Guengerich. (2007). Multiple Sequential Steps Involved in the Binding of Inhibitors to Cytochrome P450 3A4.
      J. Biological Chemistry 282, 6863-6874
Achieved using the OLIS DW2/Cary 14

T Lendrihas, GA Hunter, GC Ferreira. (2010). Serine 254 Enhances an Induced Fit Mechanism in Murine 5-Aminolevulinate Synthase .
      JBC 285, 3351-3359
Achieved using the OLIS RSM 1000F

PK Grzyska, MJ Ryle, GR Monterosso, J Liu, DP Ballou, RP Hausinger. (2006). Steady-State and Transient Kinetic Analyses of Taurine/?-Ketoglutarate Dioxygenase:? Effects of Oxygen Concentration, Alternative Sulfonates, and Active-Site Variants on the FeIV-oxo Intermediate.
      Biochemistry 44, 3845-3855
Achieved using the OLIS RSM 1000 SF

LS Motto, IB Ramos, FM Gomes, W de Souza, DE Champagne, MF Santiago, R Docampo, K Miranda, EA Machado. (2009). .
      Insect Biology and Molecular Biology 39, 198-206
Achieved using the OLIS DW2000

M Oszajca, A Franke, M Brindall, G Stochel, R van Eldik. (2011). Mechanistic Studies on the Reactions of Cyanide with a Water-Soluble Fe(III) Porphyrin and Their Effect on the Binding of NO.
      Inorg. Chem. 50, 3413-3424
Achieved using the OLIS Kinfit

KH Tang, CH Chang, PA Frey. (2001). Electron Transfer in the Substrate-Dependent Suicide Inactivation of Lysine 5,6-Aminomutase.
      Biochemistry 40, 5190-5199
Achieved using the OLIS RSM 1000

D Sun and VL Davidson. (2003). Effects of Engineering Uphill Electron Transfer into the Methylamine Dehydrogenase?Amicyanin?Cytochrome c-551i Complex.
      Biochemistry 42, 1772-1776
Achieved using the OLIS RSM 1000 SF

EA Mader and JM Mayer. (2010). The Importance of Precurser and Successor Complex Formation in a Bimolecular Proton-Electron Transfer Reaction.
      Inorg. Chem 49, 3685-3687
Achieved using the OLIS RSM 1000

WA Alves, IA Bagatin, AM Da Costa Ferreira. (2001). Equilibria and tyrosinase activity of a dinuclear and its analogous tetranuclear imidazolate-bridged copper(II) complexes.
      Inorgica Chimica Acta 321, 11-21
Achieved using the OLIS DW 2000

W Fast, Z Wang, SJ Benkovic. (2001). Familial Mutations and Zinc Stoichiometry Determine the Rate-Limiting Step of Nitrocetin Hydrolysis by Metallo-beta-lactamase from Bacterioides fragilis.
      Biochemistry 40, 1640-1650
Achieved using the OLIS Cary 14

JW Beckman, Q Wang, FP Guengerich. (2008). Kinetic Analysis of Correct Nucleotide Insertion by a Y-Family DNA Polymerase Reveals Conformational Changes Both Prior to and Following Phosphodiester Bond Conformation Formationas Directed by Tryptophan Fluorescence.
      J. Biological Chemistry 283, 36711-36723
Achieved using the OLIS DM 45

Y Zhen, B Schmidt, UG Kang, W Antholine, S Ferguson-Miller. (2002). Mutants of the CuA Site in Cytochrome c Oxidase of Rhodobacter sphaeroides: I. Spectral and Functional Properties.
      Biochemistry 41, 2288-2297
Achieved using the OLIS RSM 1000 SF

L Zhang, C-H, Tai, L Yu, C-A Yu. (2000). pH-induced Intramolecular Electron Transfer between the Iron-Sulfur Protein and Cytochrome c 1 in Bovine Cytochrome bc 1 Complex.
      J Biol Chem 275, 7656-7661
Achieved using the OLIS RSM 1000

JK Ma, Y Wang, CJ Carrell, FS Mathews, VL Davidson. (2007). A Single Methionine Residue Dictates the Kinetic Mechanism of Interprotein Electron Transfer from Methylamine Dehydrogenase to Amicyanin.
      Biochemistry 46, 11137-11146
Achieved using the OLIS RSM 1000

MR Webster, EF Johson, C Marques-Soares, S Dijols, PM Dansette, D Mansuy, CD Stout. (2003). Structure of Mammalian Cytochrome P 450 2C5 Complexed with Dichlofenac at 2.1 Angstrom Resolution: Evidence for an Induced Fit Model of Substrate Binding.
      Biochemistry 42, 9335-9345
Achieved using the OLIS DW-2

N Goyal, J Liu, L Lovings, P Dupart, S Taylor, S Bellow, L Mensah, E McClain, B Dotson, J Sridhar, X Zhang, M Zhao, M Foroozesh. (2014). Ethynylflavones, Highly Potent and Selective Inhibitors of Cytochrome P450 1A1.
      Chemical Research in Toxicology DOI:?10.1021/tx5001865
Achieved using the OLIS DM 45

Z-G Zhang, Y Liu, FP Guengerich, JH Matse, J Chen, Z-L Wu. (2009). Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries.
      J Biotech. 139, 12
Achieved using the OLIS DW2

T Sakamoto and DA Bryant. (2001). Requirement of Nickel as an Essential Micronutrient for the Utilization of Urea in the Marine Cyanobacterium.
      Microbes and Environments 16, 177-184
Achieved using the OLIS RSM 1000

D Nurizzo, HM Baker, QY He, RTA MacGillivray, AB Mason, RC Woodworth, EN Baker. (2001). Crystal Structures and Iron Release Properties of Mutants (K206A and K296A) that Abolish the Dilysine Interaction in the N-lobe of Human Transferrin.
      Biochemistry 40, 1616-1623
Achieved using the OLIS RSM 1000F

BT Farrer and HH Thorp. (2000). Redox Pathways in DNA Oxidation:? Kinetic Studies of Guanine and Sugar Oxidation by Para-Substituted Derivatives of Oxoruthenium(IV).
      Inorg. Chem. 39, 44-49
Achieved using the OLIS RSM 1000 SF

R Pietri, L Granell, A Cruz, W de Jesus, A Lewis, R Leon, CL Cadilla, JL Garriga. (2005). Tyrosine B10 and heme?ligand interactions of?Lucina pectinata?hemoglobin II: control of heme reactivity.
      BBA Proteins and Proteomics 1747, 195-203
Achieved using the OLIS RSM 1000

TC Goosen, UM Kent, L Brand, PF Hollenberg. (2000). Inactivation of Cytochrome P450 2B1 by Benzyl Isothiocyanate, a Chemopreventative Agent from Cruciferous Vegetables.
      Chem. Res. Toxicol. 13, 1349-1359
Achieved using the OLIS DW2

E Balodite, I Strazdina, N Galinina, S McLean, R Rutkis, RK Poole, U Kalnenieks. (2014). Structure of the?Zymomonas mobilisrespiratory chain: oxygen affinity of electron transport and the role of cytochrome?c?peroxidase.
      Microbiology 160, 2045-2052
Achieved using the OLIS RSM 1000

A-L Kaufholz, GA Hunter, GC Fereira, T Lendrihas, V Hering, G Layer, M Jahn, D Jahn. (2013). Aminolaevulinic acid synthase of Rhodobacter capsulatus: high-resolution kinetic investigation of the structural basis for substrate binding and catalysis.
      Biochemical Journal 451, 205-216
Achieved using the OLIS RSM 1000

S Shin, M Feng, C Li, HR Williamson, M choi, CM Wilmot, VL Davidson. (2015). A T67A mutation in the proximal pocket of the high-spin heme of MauG stabilizes formation of a mixed-valent FeII/FeIII?state and enhances charge resonance stabilization of the?bis-FeIV?state.
      Biochimica et Biophysica Acta: Bioenergetics DOI: 10.1016/j.bbabio.2015.04.008
Achieved using the OLIS RSM 1000

Z Tang, MV Martin, FP Guengerich. (2009). Elucidation of Functions of Human Cytochrome P450 Enzymes: Identification of Endogenous Substrates in Tissue Extracts Using Metabolomic and Isotope Labeling Approaches.
      Analytical Chemistry 81, 3071-3078
Achieved using the OLIS DW2

H Frauenfelder, BH McMahon, RH Austin, K Chu, JT Groves. (2001). The role of structure, energy landscape, dynamics, and allostery in the enzymatic function of myoglobin.
      PNAS 98, 2370-2374
Achieved using the OLIS Stopped flow

A Paulus, C Werner, B Ludwig, S de Vries. (2015). The cytochrome?ba3?oxidase from?Thermus thermophilus?does not generate a tryptophan radical during turnover; Implications for the mechanism of proton pumping.
      Biochimica et Biophysica Acta (BBA) Bioenergetics DOI:10.1016/j.bbabio.2015.05.013
Achieved using the OLIS DW 2000

S Harold, S Kalinga, T Matsui, Y Watanabe. (2004). Mechanistic Studies of the Isomerization of Peroxynitrite to Nitrate Catalyzed by Distal Histidine Metmyoglobin Mutants.
      JACS 126, 6945-6955
Achieved using the OLIS RSM 1000

JD Lutz, V Dixit, CK Yeung, LJ Dickmann, A Zelter, JE Thatcher, WL Nelson, N Isoherranen. (2009). Expression and functional characterization of cytochrome P450 26A1, a retinoic acid hydroxylase.
      Biochemical Pharmacology 77, 258-268
Achieved using the OLIS DW2

WD Nes, A Sinha, P Jayasimha, W Zhou, Z Song, AL Dennis. (2005). Probing the sterol binding site of soybean sterol methyltransferase by site-directed mutagenesis: Functional analysis of conserved aromatic amino acids in Region 1.
      ABB 448, 23-30
Achieved using the OLIS DSM 1000

PK Sinha, J Torres-Bacete, E Nakamaru-Ogiso, N Castro-Guerrero, A Matsuno-Yagi. (2009). Critical Roles of Subunit NuoH (ND1) in the Assembly of Peripheral Subunits with the Membrane Domain of Escherichia coli NDH-1 .
      J Biol Chem 284, 9814-9823
Achieved using the OLIS DW2000

S Neny, K Imai, H Hori, I Ishikawa, K Ishimori, D Okuno, S Nagatomo, T Hoshino, M Hata, N Funasaki. (2003). Iron Hemiporphycene as a Functional Prosthetic Group for Myoglobin.
      Inorg. Chem. 42, 1456-1461
Achieved using the OLIS RSM 1000 SF

CR Lloyd, GM Raner, A Moser, EM Eyring, WR Ellis Jr.. (2000). Oxmyohemerythrin Discriminating Between O2 Release and Autooxidation.
      J Inorganic Biochem. 81, 293-300
Achieved using the OLIS Software

AD Hegeman, JW Gross, PA Frey. (2001). Probing Catalysis by Eschrichia Coli dTDP-Glucose-4,6-dehydratase: Identification and Preliminary Characterization of Functional amino Acid Residues at the Active Site.
      Biochemistry 40, 6598-6610
Achieved using the OLIS RSM 1000

Q Cheng, CD Sohl, FF Yoshimoto, FP Guengerich. (2012). Oxidation of Dihydrotestosterone by Human Cytochromes P450 19A1 and 3A4.
      J. Biol. Chem. 287, 29554-29567
Achieved using the OLIS DW 2000

H Tian, R Guan, E Salsi, B Campanini, S Battati, V Prasana Kumar, WE Karsten, A Mozzarelli, PF Cook. (2010). Identification of the Structural Determinants for the Stability of Substrate and Aminoacrylate External Schiff Bases in O-Acetylserine Sulfhydrylase-A.
      Biochemistry 49, 6093-6103
Achieved using the OLIS RSM 1000

A Dementiev, R Swanson, R Roth, G Isetti, G Izaguirre, ST Olson, PGW Gettins. (2013). The allosteric mechanism of activation of antithrombin as an inhibitor of factor IXa and factor Xa. Heparin-independent full activation through mutations adjacent to helix D..
      Journal of Biological Chemistry 288, 33611-33619
Achieved using the OLIS SLM 8000

I Smirnova, V Kasho, J Sugihara, HR Kaback. (2013). Trp replacements for tightly interacting Gly-Gly pairs in LacY stabilize an outward-facing conformation..
      Proc. Natl. Acad. Sci. 110, 8876-8881
Achieved using the OLIS SLM 8000 SF

CW Locuson, PM Gannett, TS Tracy. (2006). Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9.
      ABB 449, 115-129
Achieved using the OLIS DW2000

II Protasevich, CG Brouillette, ME Snow, S Dunham, JR Rubin, R Gogliotti, K Siegel. (2004). Role of Inhibitor Aliphatic Chain in the Thermodynamic of Inhibition Binding to Escherichia coli: Enoyl-ACP Reductase and the Phe 203Leu Mutant: A Proposed Mechanism for Drug Resistance.
      Biochemistry 43, 13380-13389
Achieved using the OLIS DSM 1000

D-L Popescu, M Vrabel, A Brausam, P Madsen, G Lente, I Fabian, AD Ryabov, R van Eldik, TJ Collins. (2010). Thermodynamic, Electrochemical, High-Pressure Kinetic, and Mechanistic Studies of the Formation of Oxo FeIV?TAML Species in Water.
      Inorg. Chem. 49, 11439-11448
Achieved using the OLIS KinFit

CL Quinlan, AL Carr, IV Perevoshchikova, JR Treberg, BA Ackrell, MD Brand. (2012). Mitochondrial complex II can generate reactive oxygen species at high rates in both the forward and reverse reactions.
      J. Biol. Chem. 287, 27255-27264
Achieved using the OLIS DW2000

Y Yang, T Yamashita, E Nakamura-Ogiso, T Hashimoto, M Murai, J Igarashi, H Miyoshi, N Mori, A Matsuno-Yagi, T Yagi, H Kosaka. (2010). Reaction Mechanism of Single Subunit NADH-Ubiquinone Oxidoreductase (Ndi1) from Saccharomyces cerevisiae:Evidence for a ternary complex mechanism.
      J. Biol. Chem. 286, 9287-9297
Achieved using the OLIS DW2000

CH Li and SC Tu. (2005). Probing the Functionalities of ?Glu328 and ?Ala74 of Vibrio harveyi Luciferase by Site-Directed Mutagenesis and Chemical Rescue.
      Biochemistry 44, 13866-13873
Achieved using the OLIS RSM 1000 SF

IV Vrcek and G Sinko. (2013). Inactivation of Cholinesterases by Silver and Gold Ions in vitro.
      Central European Journal of Chemistry 11, 935-944
Achieved using the OLIS RSM 1000

FI Rosel, HH Kuo, AG Mauk. (2011). NADH-Oxidase Activity of Indoleamine 2,3-Dioxygenase.
      J. Biol. Chem. 286, 29273-29283
Achieved using the OLIS RSM 1000

AS Pawate, J Morgan, A Namslauer, D Mills, P Brzezinski, S Ferguson-Miller. RB Gennis. (2002). A Mutation in Subunit I of Cytochrome Oxidase from Rhodobacter sphaeroides Results in an Increase in Steady-State Activity but Completely Eliminates Proton Pumping.
      Biochemistry 41, 13417-13423
Achieved using the OLIS RSM 1000 SF

N Gupta and SW Ragsdale. (2008). Dual Roles of an Essential Cysteine Residue in Activity of a Redox-regulated Bacterial Transcriptional Activator .
      J Biol Chem 283, 28721-28728
Achieved using the OLIS RSM 1000F

J Liu, SF Taylor, PS Dupart, CL Arnold, J Sridhar, Q Jiang, Y Wang, EV Skriprikova, M Zhao, M Foroozesh. (2013). Pyranoflavones: A Group of Small-molecule Probes for Exploring the Active Site Cavities of Cytochrome P450 Enzymes 1A1, 1A2, and 1B1.
      J. Med. Chem. 56, 4082-4092
Achieved using the OLIS DM 45

JP Emerson, ED Coulter, RS Phillips, DM Kurtz Jr.. (2003). Kinetics of the Superoxide Reductase Catalytic Cycle.
      J Biol Chem 278, 39662-39668
Achieved using the OLIS RSM 1000F

PJ Halbrooks, QY He, SK Briggs, SJ Everse, VC Smith, RTA MacGillivray. (2003). Investigation of the Mechanism of Iron Release from the C-Lobe of Human Serum Transferrin:? Mutational Analysis of the Role of a pH Sensitive Triad.
      Biochemistry 42, 3701-3707
Achieved using the OLIS RSM 1000

AC Raffalt, L Schmidt, HEM Christensen, Q chi, J Ulstrup. (2009). Electron transfer patterns of the di-heme protein cytochrome?c4?fromPseudomonas stutzeri.
      J Inorg. Chem. 103, 717-722
Achieved using the OLIS Hi Tech SF 53

FP Guengerich, JL Sorrells, S Schmitt, JA Krauser, P Aryal, L Meijer. (2004). Generation of New Protein Kinase Inhibitors Inhibitors Utilizing Cytochrome P450 Mutant Enzymes for Indigoid Synthesis.
      J. Med. Chem. 47, 3236-3241
Achieved using the OLIS Cary 14

DB Northrop, Y-K Cho. (2000). Effects of High Pressure on Solvent Isotope Effects of Yeast Alcohol Dehydrogenase.
      Biophysical J 79, 1621-1628
Achieved using the OLIS DW2000

M Castellani, R Covian, T Kleinschroth, O Anderkay, B Ludwig, BL Trumpower. (2010). Direct Demonstration of Half-of-the-sites Reactivity in the Dimeric Cytochrome bc1 Complex: ENZYME WITH ONE INACTIVE MONOMER IS FULLY ACTIVE BUT UNABLE TO ACTIVATE THE SECOND UBIQUINOL OXIDATION SITE IN RESPONSE TO LIGAND BINDING AT THE UBIQUINONE REDUCTION SITE .
      JBC 285, 502-510
Achieved using the OLIS RSM 1000

M Gutierrez-Aguilar, V Perez-Vazquez, O Bunoust, S Manon, M Rigoulet, S Uribe. (2007). In yeast, Ca2+?and octylguanidine interact with porin (VDAC) preventing the mitochondrial permeability transition.
      BBA Bioenergetics 1767, 1245-1251
Achieved using the OLIS DW2000

CH Li and SC Tu. (2005). Active Site Hydrophobicity Is Critical to the Bioluminescence Activity of Vibrio harveyi Luciferase.
      Biochemistry 44, 12970-12977
Achieved using the OLIS RSM 1000 SF

M Fabian and G Palmer. (2001). Proton Involvement in the Transition from the "Peroxy" to the Ferryl Intermediate of Cytochrome C Oxidase.
      Biochemistry 40, 1867-1878
Achieved using the OLIS DOS software

ZL Wu, LM Podust, FP Guengerich. (2005). Expansion of Substrate Specificity of Cytochrome P450 2A6 by Random and Site Directed Mutagenesis.
      J. Biological Chemistry 280, 41090-41100
Achieved using the OLIS DW2/Cary 14

KJ Koebke, DJ Pauly, L Lerner, X Liu, AA Pacheco. (2013). Does the Oxidation of Nitric Oxide by oxyMyoglobin Share an Intermediate with the metMyoglobin-Catalyzed Isomerization of Peroxynitrite?.
      Inorganic Chemistry 52, 7623-7632
Achieved using the OLIS RSM 1000

R Shinkyo and FP Guengerich. (2011). Inhibition of Human Cytochrome P450 3A4 by Cholesterol.
      J. Biol. Chem. 286, 18426-18433
Achieved using the OLIS RSM 1000

MD Vlasie and R Banerjee. (2004). When a Spectator Turns Killer: Suicidal Electron Transfer from Cobalamin in Methylmalonyl-CoA Mutase.
      Biochemistry 43, 8410-8417
Achieved using the OLIS SF?

PS David and RO Poyton. (2006). Effects of a transition from normoxia to anoxia on yeast cytochrome?c?oxidase and the mitochondrial respiratory chain: Implications for hypoxic gene induction.
      BBA Bioenergetics 1709, 169-180
Achieved using the OLIS DW 2000

R van Lis, D Gonzalez-Halphen, A Atteia. (2005). Divergence of the mitochondrial electron transport chains from the green algaChlamydomonas reinhardtii?and its colorless close relative?Polytomella?sp..
      BBA Bioenergetics 1708, 23-34
Achieved using the OLIS DW2

E Nickel, K Nienhaus, C Lu, S-R, Yeh, GU Nienhaus. (2009). Ligan and Substrate Migration in Human Indoleamine 2,3-Dioygenase.
      JBC 284, 31548-31554
Achieved using the OLIS Cary 14

A Zapata, MR Kumar, D Pervitsky, PJ Farmer. (2012). A singular variable decomposition approach for kinetic analysis of reactions of HNO with myoglobin.
      J. Inorganic Biochemistry 118, 171-178
Achieved using the OLIS RSM 1000

SA Seibold, T Ball, LC His, DA Mills, RD Abeysinghe, R Micielli, CJ Rieke, RI Cukier, WL Smith. (2003). Histidine 386 and Its Role in Cyclooxygenase and Peroxidase Catalysis by Prostaglandin-endoperoxide H Synthases .
      J Biol Chem 278, 46163-46170
Achieved using the OLIS RSM 1000

PM Gannett, J Kabulski, FA Perez, D Lederman, CW Locuson, RK Ayscue, NM Thomsen, TS Tracy. (2006). Preparation, Characterization, and Substrate Metabolism of Gold-Immobilized Cytochrome P450 2C9.
      JACS 128, 8374-8375
Achieved using the OLIS DW 2000

ZL Wu, P Aryal, O Lazach, L Meijer, FP Guengerich. (2005). Biosynthesis of New Indigoid Inhibitors of Protein Kinases Using Recombinant Cytochrome P450 2A6.
      Chem. & Biodiversity 2, 51-65
Achieved using the OLIS DW2/Cary 14

K Kumar and FG Walz, Jr.. (2001). Probing Functional Perfection in Substructures of Ribonuclease T1:? Double Combinatorial Random Mutagenesis Involving Asn43, Asn44, and Glu46 in the Guanine Binding Loop.
      Biochemistry 40, 3748-3757
Achieved using the OLIS Cary 118

SP Balashov, ES Imasheva, AR Choi, K -H Jung, S Liaaen-Jensen, JK Lanyi. (2010). Reconstitution of Gloeobacter Rhodopsin with Echinenone: Role of the 4-keto Group.
      Biochemistry 16, 9792-9799
Achieved using the OLIS SLM

J Zhang and GC Ferreira. (2002). Transient State Kinetic Investigation of 5-Aminolevulinate Synthase Reaction Mechanism .
      J Biol Chem 277, 44660-44669
Achieved using the OLIS RSM 1000F

MT Cash, EW Miles, RS Phillips. (2004). The reaction of indole with the aminoacrylate intermediate of Salmonella typhimurium tryptophan synthase: observation of a primary kinetic isotope effect with 3-[2H]indole.
      ABB 432, 233-243
Achieved using the OLIS RSM 1000

RS Phillips, R Ghaffari, P Dinh, S Lima, D Bartlett. (2010). Properties of tryptophan indole-lyase from a piezophilic bacterium,Photobacterium profundum?SS9.
      Arch. Biochem. Biophys 506, 35-41
Achieved using the OLIS RSM 1000

E Karasik, LD Kwiatkowski, HL Hui, JF Colby, RW Noble. (2004). Effects of Heterotropic Allosteric Effectors on the Equilibrium and Kinetics of the Reactions of a Single Ligand Molecule with an alpha or beta Subunit of Deoxygenated HbA.
      Biochemistry 43, 7851-7856
Achieved using the OLIS Cary 14

T Wenz, R Covian, P Hellwig, F MacMillen, B Meunier, BL Trumpower, C Hunte. (2007). Mutational Analysis of Cytochrome b at the Ubiquinol Oxidation Site of Yeast Complex III.
      J Biol Chem 282, 3977-3988
Achieved using the OLIS RSM 1000

RS Phillips, HY Chen, NG Faleev. (2006). Aminoacrylate Intermediates in the Reaction of Citrobacter freundii Tyrosine Phenol-Lyase.
      Biochemistry 45, 9575-9583
Achieved using the OLIS RSM/Cary 14

P Pavel and A Tiwari. (2010). Differential mechanism of light-induced and oxygen-dependent restoration of the high-potential form of cytochrome b559 in Tris-treated Photosystem II membranes?.
      BBA Bioenergetics 1797, 451
Achieved using the OLIS RSM 1000

VV Smirnov, DW Brinkley, MP Lanci, KD Karlin, JP Roth. (2006). Probing metal-mediated O2?activation in chemical and biological systems.
      J Molecular Catalysis A: Chemical 251, 100-107
Achieved using the OLIS RSM 1000

AL Orr, CL Quinlan, IV Perevoshchikova, MD Brand. (2012). A Refined Analysis of Superoxide Production by Mitochondrial sn-Glycerol 3-Phosphate Dehydrogenase.
      J. Biol. Chem. 287, 42921-42935
Achieved using the OLIS DW2000

AP Batista, BC Marreiros, MM Perreira. (2011). Decoupling of catalytic and transport activities of Rhodothermus marinus complex I by a sodium/proton antiporter inhibitor.
      ACS Chem. Biol. 6, 477-483
Achieved using the OLIS DW 2

S Wu, G Wu, KK Surerus, JA Cowan. (2002). Iron-Sulfur Cluster Biosynthesis. Kinetic Analysis of [2Fe-2S] Cluster Transfer from Holo ISV to Apo Fd: Role of Redox Chemistry and a Conserved Aspartate.
      Biochemistry 41, 8876-8885
Achieved using the OLIS RSM and HPDA

KP Neupane, K Gearty, A Francis, J Shearer. (2007). Probing Variable Axial Ligation in Nickel Superoxide Dismutase Utilizing Metallopeptide-Based Models:? Insight into the Superoxide Disproportionation Mechanism.
      JACS 129, 14605-14618
Achieved using the OLIS Cary 14/DSM 17

M Oszajca, A Franke, A Drzewiecka-Matuszek, M Brindell, G Stochel, R van Eldik. (2014). Temperature and Pressure Effects on C?H Abstraction Reactions Involving Compound I and II Mimics in Aqueous Solution.
      Inorganic Chemistry 53, 2848-2857
Achieved using the OLIS Kinfit

AL Blobaum, Y Lu, UM Kent, S Wang, PF Holenberg. (2004). Formation of a Novel Reversible Cytochrome P450 Spectral Intermediate:? Role of Threonine 303 in P450 2E1 Inactivation.
      Biochemistry 43, 11942-11952
Achieved using the OLIS DW2

N Song and DM Stanbury. (2011). Overoxidation of Phenol by Hexachloroiridate(IV).
      Inorg. Chem. 50, 12762-12773
Achieved using the OLIS Hi Tech SF 53

IH Hanna, JA Krauser, H Cai, MS Kim, FP Guengerich. (2001). Diversity in Mechanisms of Substrate Oxidation by Cytochrome P450 2D6: Lack of an Allosteric Role of NADPH-Cytochrome P450 Reductase in Catalytic Regioselectivity.
      J. Biological Chemistry 276, 39553-39561
Achieved using the OLIS DW2

H Zang, Q Fang, AE Pegg, FP Guengerich. (2005). Kinetic Analysis of Steps in the Repair of Damaged DNA by Human 06-alkylguanine DNA Alkyltransferase.
      J. Biological Chemistry 280 30873-30881
Achieved using the OLIS DM 45

J Seravalli, M Kumar, SW Ragsdale. (2002). Rapid Kinetic Studies of Acetyl-CoA Synthesis: Evidence Supporting the Catalytic Intermediacy of a Paramagnetic NiFeC Species in the Autotropic Wood-Ljungdahl Pathway.
      Biochemistry 41, 1807-1819
Achieved using the OLIS Cary 14

DA Geisler, C Broselid, L Hederstedt, AG Rasmusson. (2007). Ca2+-binding and Ca2+-independent Respiratory NADH and NADPH Dehydrogenases of Arabidopsis thaliana .
      J Biol Chem 282, 28455-28464
Achieved using the OLIS DW2

CH Snyder, EB Gutierrez-Cirlos, BL Trumpower. (2000). Evidence for a Concerted Mechanism of Ubiquinol Oxidation by the Cytochrome bc 1 Complex .
      J Biol Chem 275, 13535-13541
Achieved using the OLIS RSM 1000

OI Gogoleva, LN Zakomirdina, TV Demidkina, RS Phillips, NG Faleev. (2003). Tryptophanase in aqueous methanol: the solvent effects and a probable mechanism of the hydrophobic control of substrate specificity.
      Enzyme and Micropbial Technology 32, 843-850
Achieved using the OLIS SIFIT

VB Gawandi, D Liskey, S Lima, RS Phillips. (2004). Reaction of Pseudomonas fluorescens Kynureninase with ?-Benzoyl-l-alanine:? Detection of a New Reaction Intermediate and a Change in Rate-Determining Step.
      Biochemistry 43, 3230-3237
Achieved using the OLIS RSM 1000 SF

CJ Chang, ZH Loh, C Shi, FC Anson, DG Nocera. (2004). Targeted Proton Delivery in the Catalyzed Reduction of Oxygen to Water by Bimetallic Pacman Porphyrins.
      JACS 126, 10013-10020
Achieved using the OLIS Cary 17

A Boullier, JC Maziere, P Filipe, LK Patterson, DM Bartels, GL Hug, JP Freitas, R Santus, P Morliere. (2007). Interplay of Oxygen, Vitamin E, and Carotenoids in Radical Reactions Following Oxidation of Trp and Try Residues in Native HDL3 Apolipoproteins. Comparison with LDL. A Time-Resolved Spectroscopic Analysis.
      Biochemistry 46, 5226-5237
Achieved using the OLIS RSM 1000

A Weichsel, EM Maes, JF Anderson, JG Valenzuela, TK Shokhireva, FA Walker, WR Monfort. (2005). Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein .
      PNAS 102, 594-599
Achieved using the OLIS RSM 1000

T Li, JK Ma, JP Hosler, VL Davidson, A Liu. (2007). Detection of Transient Intermediates in the Metal Dependent Nonoxidative Decarboxylation Catalyzed by ?-Amino-?-Carboxymuconate-?-Semialdehyde Decarboxylase.
      JACS 129, 9278-9279
Achieved using the OLIS RSM 1000 SF

NR Mattatall, LM Cameron, BC Hill. (2001). Transient-State Reduction and Steady-State Kinetic Studies of Menaquinol Oxidase from Bacillus Subtilis, Cytochrome aa3-600 nm Spectroscopic Characterization of the Steady-State Species.
      Biochemistry 40, 13331-13341
Achieved using the OLIS DSM 1000

J Pearson, UP Dahal, D Rock, C-C Peng, JO Schenk, C Joswig-Jones, JP Jones. (2011). The kinetic mechanism for cytochrome P450 metabolism of Type II binding compounds: Evidence supporting direct reduction.
      Arch. Biochem. Biophys. 511, 69-79
Achieved using the OLIS DW2000

RCJ Dobson, SRA Devenish, LA Turner, VR Clifford, FG Pearce, GB Jameson, JA Gerrard. (2005). Role of Arginine 138 in the Catalysis and Regulation of Escherichia coli Dihydrodipicolinate Synthase.
      Biochemistry 44, 13007-13013
Achieved using the OLIS DSM 10

D Kim, GS Cha, LD Nagy, C-H Yun, FP Guengerich. (2014). Kinetic Analysis of Lauric Acid Hydroxylation by Human Cytochrome P450 4A11.
      Biochemistry 53, 6161-6172
Achieved using the OLIS DW 2000

AP Harris and RS Phillips. (2013). Benzimidazole Analogues of L-Tryptophan are Substrates and Inhibitors of Tryptophan Indole-Lyase from?Escherichia coli.
      FEBS Journal 280, 1807-1817
Achieved using the OLIS RSM 1000

LB von Weyman, AL Blobaum, PF Hollenberg. (2004). The mechanism-based inactivation of P450 2B4 by tert-butyl 1-methyl-2-propynyl ether: structural determination of the adducts to the P450 heme.
      ABB 425, 95-105
Achieved using the OLIS DW2

E Pierce, DF Becker, SW Ragsdale. (2010). Identification and Characterization of Oxalate Oxidoreductase, a Novel Thiamine Pyrophosphate-dependent 2-Oxoacid Oxidoreductase That Enables Anaerobic Growth on Oxalate.
      JBC 285, 40515-40524
Achieved using the OLIS Cary 14

H Zang, TM Harris, FP Guengerich. (2005). Kinetics of Nucleotide Incorporation Opposite DNA Bulky Guanine N2 Adducts by Processive Bacteriophage T7 DNA Polymerase (Exonuclease) and HIV-1 Reverse Transcriptase.
      J. Biological Chemistry 280 1165-1178
Achieved using the OLIS Cary 14

J Sridhar, P Jin, J Liu, M Faroozesh, CL Klein Stevens. (2010). In Silico Studies of Polyaromatic Hydrocarbon Inhibitors of Cytochrome P450 Enzymes 1A1, 1A2, 2A6, and 2B1.
      Chem Res Toxicol 23, 600-607
Achieved using the OLIS DM 45

MM Pereira, FL Sousa, M Teixeira, RM Nyquist, J Heberle. (2006). A tyrosine residue deprotonates during oxygen reduction by the?caa3 reductase from?Rhodothermus marinus.
      FEBS Lett. 580, 130-1354
Achieved using the OLIS DW2

KP Conner, P Vennam, MD Krzyaniak, CM Woods, MK Bowman, WM Atkins. (2012). 1,2,3-Triazole-Heme Interactions in Cytochrome P450: Functionally Competent Triazole-Water-Heme Complexes.
      Biochemistry 51, 6441-6457
Achieved using the OLIS DW 2

Y Tsukatani, SP Romberger, JH Golbeck, DA Bryant. (2011). Isolation and characterization of a homodimeric type-I reaction center complex from Candidatus Chloracidobacterium thermophilum, an aerobic chlorophototroph.
      J. Biol. Chem. 287, 5720-5732
Achieved using the OLIS Cary 14

GA Hunter, E Rivera, GC Ferreira. (2005). Supraphysiological concentrations of 5-aminolevulinic acid dimerize in solution to produce superoxide radical anions via a protonated dihydropyrazine intermediate.
      ABB 437, 128-137
Achieved using the OLIS RSM 1000F

QT Do, GT Nguyen, V Celis, RS Phillips. (2014). Inhibition of?Escherichia coli?tryptophan indole-lyase by tryptophan homologues.
      Archives of Biochemistry and Biophysics 560, 20-26
Achieved using the OLIS RSM 1000

S Gomez-Manzo, M Contreras-Zentella, a Gonzalez-Valden, M Sosa-Torres, R Arreguin-Espinoza, E Escamilla-Marvan. (2008). The PQQ-alcohol dehydrogenase of?Gluconacetobacter diazotrophicus.
      Int J Food Microbiology 125, 71-78
Achieved using the OLIS DW2000

V Bandarian and GH Reed. (2000). Isotope Effects in the Transient Phases of the Reaction Catalyzed by Ethanolamine Ammonia-Lyase: Determination of the Number of Exchangeable Hydrogens in the Enzyme-Cofactor Complex.
      Biochemistry 39, 12069-12075
Achieved using the OLIS RSM 1000

LA Solomon, G Kodali, CC Moser, PL Dutton. (2014). Engineering the assembly of heme cofactors in man-made proteins.
      Journal of the American Chemical Society DOI: 10.1021/ja411845f
Achieved using the OLIS RSM 1000

I Azarov, C Liu, H Reynolds, Z Tsekouras, JS Lee, MT Gladwin, DB Kim-Shapiro. (2011). Mechanisms of slower nitric oxide uptake by red blood cells and other hemoglobin-containing vesicle.
      J. Biol.Chem. 286, 33567-33579
Achieved using the OLIS RSM 1000

D Jancura, V Berka, M Antalik, J Bagelova, RB Gennis, G Palmer, M Fabian. (2006). Spectral and Kinetic Equivalence of Oxidized Cytochrome c Oxidase as Isolated and ?Activated? by Reoxidation.
      J Biol Chem 281, 30319-31325
Achieved using the OLIS RSM 1000

R Wedmann, S Bertlein, I Macinkovic, S Bolz, J Miljkovic, L Munoz, M Herrmann, MR Filipovic. (2014). Working With ?H2S?: Facts and apparent artifacts.
      Nitric Oxide 41, 85-96
Achieved using the OLIS HPDA 8452

D Jancura, M Antalik, V Berka, G Palmer, M Fabian. (2006). Filling the Catalytic Site of Cytochrome c Oxidase with Electrons: REDUCED CuB FACILITATES INTERNAL ELECTRON TRANSFER TO HEME a3 .
      J Biol Chem 281, 20003-20010
Achieved using the OLIS RSM 1000

LD Kwiatkowski, HL Hui, E Karasik, JE Colby, RW Noble. (2007). Mutations of the ?N102 Residue of HbA Not Only Inhibit the Ligand-Linked T to Re State Transition, but Also Profoundly Affect the Properties of the T State Itself.
      Biochemistry 46, 2037-2049
Achieved using the OLIS Cary 14

S Daum, CH Tai, PF Cook. (2003). Characterization of the S272A, D Site-Directed Mutations of O-Acetylserine Sulhydrylase: Involvement of the Pyridine Ring in the alpha, beta Elimination Reaction.
      Biochemistry 42, 106-113
Achieved using the OLIS RSM 1000

RJ Auchus, AS Kumar, CA Boswell, MK Gupta, K Bruce, NP Rath, DF Covey. (2003). The enantiomer of progesterone (ent-progesterone) is a competitive inhibitor of human cytochromes P450c17 and P450c21.
      ABB 409, 134-144
Achieved using the OLIS DW2

JW Gross, AD Hegeman, MM Vestling, PA Frey. (2000). Characterization of Enzymatic Processes by Rapid Mix-Quench Mass Spectrometry: The Case of dTDP-Glucose 4,6-Dehydratase.
      Biochemistry 39, 13633-13640
Achieved using the OLIS RSM 1000

FP Guengerich, GP Miller, IH Hanna, MV Martin, S Lege, C Black, N Chauret, JM Siha, LA Trimble, JA Yergey, DA Nicoll-Griffith. (2002). Diversity in the Oxidatiof Substrates by Cytochrome P450 2D6: Lack of an Obligatory Role of Aspartate 301-Substrate Electrostatic Binding.
      Biochemistry 41, 11025-11034
Achieved using the OLIS DW2/Cary 14

K Rahman, MAF Abdullah, S Ambati, MD Taylor, MJ Adang. (2012). Differential protection of Cry1Fa toxin by cadherin orthologs against Spodoptera frugiperda larval gut proteases correlates with increased synergism.
      Applied and Environmental Microbiology 78, 354-362
Achieved using the OLIS RSM 1000

Z -L Wu, CJ Bartleson, A -JL Ham, FP Guengerich. (2006). Heterologous expression, purification, and properties of human cytochrome P450 27C1 .
      ABB 445, 138-146
Achieved using the OLIS DW2

D Barrick, NT Ho, V Simplaceanu, C Ho. (2001). Distal Ligand Reactivity and Quaternary Structure Studies of Proximally Detached Hemoglobins.
      Biochemistry 40, 3780-3795
Achieved using the OLIS Stopped flow

MS Afanasyen, MB Taraban, NE Polyakov, PA Purtov, TV Leshina, CB Grissom. (2006). Magnetic Spin Effects in Enzymatic Reactions:? Radical Oxidation of NADH by Horseradish Peroxidase.
      JACS 128, 8651-8658
Achieved using the OLIS RSM 1000 SF

S Herold. (2004). The Outer-Sphere Oxidation of Nitrosyliron(II)hemoglobin by Peroxynitrite Leads to the Release of Nitrogen Monoxide.
      Inorg. Chem. 43, 3783-3785
Achieved using the OLIS RSM 1000 SF

EJ Fogle and WA van der Donk. (2007). Pre-Steady State Studies of Phosphite Deydrogenase Demonstrate That Hydride Transfer is Fully Rate Limiting.
      Biochemistry 46, 13101-13108
Achieved using the OLIS RSM 1000

J Yang, S Rawat, TL Stemmler, and BP Rosen. (2010). Arsenic Binding and Transfer by the As(III) Metallochaperone.
      Biochemstry 4, 3658-3666
Achieved using the OLIS DSM 16

ZM Beharry, DM Elby, ED Coulter, R Viswanathans, EL Neidle, RS Phillips, DM Kurtz, Jr.. (2003). Histidine Ligand Protonation and Redox Potential in the Rieske Dioxygenases:? Role of a Conserved Aspartate in Anthranilate 1,2-Dioxygenase.
      Biochemistry 42, 13625-13636
Achieved using the OLIS RSM 1000 SF

Y Cai and JH Espenson. (2005). Catalysis by Methyltrioxorhenium(VII):? Reduction of Hydronium Ions by Europium(II) and Reduction of Perchlorate Ions by Europium(II) and Chromium(II).
      Inorg. Chem. 44, 489-495
Achieved using the OLIS RSM 1000 SF

S Langoeut, LL Furge, N Keriguy, K Nakamura, A Guillouzo, FP Guengerich. (2000). Inhibition of Human Cytochrome P450 Enzymes by 1,2-Dithiole-3-thione, Oltipraz and its Derivatives and Sulforaphane.
      Chem. Res. Toxicology 13, 245-252
Achieved using the OLIS DW2

AG Roberts, J Yang, JR Halpert, SD Nelson, KT Thummel, WM Atkins. (2011). The Structural Basis for Homotropic and Heterotropic Cooperativity of Midazolam Meabolism by Human Cytochrome P450 3A4.
      Biochemistry 50, 10801-10818
Achieved using the OLIS DW2

C Fengm HL Wilson, JK Hurley, JT Hazzard, G Tollin, KV Rajagopalan, JH Enemark. (2003). Essential Role of Conserved Arginine 160 in Intramolecular Electron Transfer in Human Sulfite Oxidase.
      Biochemistry 42, 12235-12242
Achieved using the OLIS RSM 1000

PP Ghoroghchian, PR Frail, K Susumu, D Blessington, AK Brannan, FS Bates, B Chance, DA Hammer, MJ Therien. (2005). Near-infrared-emissive polymersomes: Self-assembled soft matter for in vivo optical imaging .
      PNAS 102, 2922-2927
Achieved using the OLIS RSM 1000

M Choi, S Shin, VL Davidson. (2012). Characterization of electron tunneling and hole hopping reactions between different forms of MauG and methylamine dehydrogenase within a natural protein complex.
      Biochemistry 51, 6942-6949
Achieved using the OLIS RSM 1000

OL Guryex, AA Gilep, SA Usanov, RW Estabrook. (2001). Interaction of Apo-cytochrome b5 with Cytochrome P450A4 and P45017A: Relevance of Heme Transfer Reactions.
      Biochemistry 40, 5018-5031
Achieved using the OLIS RSM 1000

W Shi, J Mersfelder, R Hille. (2005). The Interaction of Trimethylamine Dehydrogenase and Electron-transferring Flavoprotein .
      J Biol Chem 280, 20239-20246
Achieved using the OLIS Kinetic Instruments SF

CE Sabel, R Carbone, JR Dabous, SY Lo, S Siemann. (2011). Preparation and characterization of cobalt-substituted anthrax lethal factor.
      Biophys. Biochem. Res. Comm. 16, 106-110
Achieved using the OLIS RSM 1000

AB Daugherty, S Govindarajan, S Lutz. (2013). Improved biocatalysts from a synthetic circular permutation library of the flavin-dependent oxidoreductase Old Yellow Enzyme.
      J. American Chemical Society 135, 14425-14432
Achieved using the OLIS RSM 1000

J Sridhar, J Liu, M Foroozesh, CK Stevens. (2011). Inhibition of Cytochrome P450 Enzymes by Quinones and Anthraquinones.
      Chem. Res. Toxicol. 25, 357-365
Achieved using the OLIS DM 45

H Rottenberg, R Covian, BL Trumpower. (2009). Membrane Potential Greatly Enhances Superoxide Generation by the Cytochrome bc1 Complex Reconstituted into Phospholipid Vesicles .
      J Biol Chem 284, 19203-19210
Achieved using the OLIS RSM 1000

BJ Chitester and FG Walz. (2002). Kinetic studies of guanine recognition and a phosphate group subsite on ribonuclease T1 using substitution mutants at Glu46 and Lys41.
      ABB 406, 73-77
Achieved using the OLIS Cary 118

T Yamashita, E Nakamaru-Ogioso, H Miyoshi, A Matsuno-Yagi, T Yagi. (2007). Roles of Bound Quinone in the Single Subunit NADH-Quinone Oxidoreductase (Ndi1) from Saccharomyces cerevisiae .
      J Biol Chem 282, 6012-6020
Achieved using the OLIS DW2000

S Gomez-Manzo, A Solano-Peralta, JP Saucedo-Vazquez, JE Escamilla-Marvan, PMH Kroneck, ME Sosa-Torres. (2010). The Membrane-Bound Quinohemoprotein Alcohol Dehydrogenase from Gluconacetobacter diazotrophicus PAL5 Carries a [2Fe-2S] Cluster.
      Biochemistry 49, 2409-2415
Achieved using the OLIS DW2000

C Furdui and SW Ragsdale. (2002). The Roles of Coenzyme A in the Pyruvate:Ferredoxin Oxidoreductase Reaction Mechanism:? Rate Enhancement of Electron Transfer from a Radical Intermediate to an Iron?Sulfur Cluster.
      Biochemistry 41, 9921-9937
Achieved using the OLIS RSM 1000 SF

M Antalik, D Jancura, G Palmer, M Fabian. (2005). A Role for Protein in Inernal Electron Transfer to the Catalytic Center of Cytochrome c Oxidase.
      Biochemistry 44, 14881-14889
Achieved using the OLIS RSM 1000

FI Rosell, TR Harris, DP Heldebrand, S Dopner, P Hildebrandt, AG Mauk. (2000). Characterization of an Alkaline Transition Intermediate Stabilized in the Phe82Trp Variant of Yeast iso-1-Cytochrome c.
      Biochemistry 39, 9047-9054
Achieved using the OLIS RSM 1000 SF

WE Karsten, T Ohshiro, Y Izumi, PF Cook. (2005). Reaction of Serine-Glyoxylate Aminotransferase with the Alternative Substrate Ketomalonate Indicates Rate-Limiting Protonation of a Quinonoid Intermediate.
      Biochemistry 44, 15930-15936
Achieved using the OLIS RSM 1000 SF

LA Luevano-Martinez, C Barba-Ostria, D Araiza-Olivera, N Chiquete-Felix, S Georgellis, S Uribe-Carvajal. (2012). A CRITICAL TYROSINE RESIDUE DETERMINES THE UNCOUPLING PROTEINLIKE ACTIVITY OF THE YEAST MITOCHONDRIAL OXALOACETATE CARRIER.
      Biochemical Journal Immediate Publication Manuscript BJ20110992
Achieved using the OLIS DW2

AG Roberts and WM Atkins. (2007). Energetics of heterotropic cooperativity between ?-naphthoflavone and testosterone binding to CYP3A4.
      ABB 463, 89-101
Achieved using the OLIS DW2

C Everroad, C Six, F Partensky, J-C Thomas, J Holtzendorf. (2006). Biochemical Bases of Type IV Chromatic Adaption in Marine synchococcus spp..
      J Bacteriol. 188, 3345-3356
Achieved using the OLIS DW2

A Mukherjee, AM Angeles-Boza, GS Huff, JP Roth. (2011). Catalytic Mechanism of a Heme and Tyrosyl Radical-Containing Fatty Acid ?-(Di)oxygenase.
      JACS 133, 227-238
Achieved using the OLIS RSM 1000

AL Blobaun, UM Kent, WL Alworth, PF Hollenberg. (2002). Mechanism-Based Inactivation of Cytochromes P450 2E1 and 2E1 T303A by tert-Butyl Acetylenes:? Characterization of Reactive Intermediate Adducts to the Heme and Apoprotein.
      Chem. Res. Toxicol. 15, 1561-1571
Achieved using the OLIS DW2

X Xiao, R Shinkyo, FP Guengerich. (2011). Cytochrome P450 2S1 is Reduced by NADPH-Cytochrome P450 Reductase.
      Drug. Met. Disposition 39, 944-946
Achieved using the OLIS Cary 14

A Lena-Kaufholz, GA Hunter, GC Ferreira, T Lendrihas, V Hering, G Layer, M Jahn, D Jahn. (2013). Aminolevulinic Acid Synthase of?Rhodobacter capsulatus?- High Resolution Kinetic Investigation of the Structural Basis for Substrate Binding and Catalysis.
      Biochemical Journal 451, 205-216
Achieved using the OLIS RSM 1000

I Efimov, CN Cronin, DJ Bergmann, V Kuusk, WS McIntire. (2004). Insight into Covalent Flavinylation and Catalysis from Redox, Spectral, and Kinetic Analyses of the R474K Mutant of the Flavoprotein Subunit of p-Cresol Methylhydroxylase.
      Biochemistry 43, 6138-6148
Achieved using the OLIS RSM 1000 SF

C Marques-Soarers, S Dijols, AC Macherey, MR Webster, EF Johnson, PM Dansette, and D Mansuy. (2003). Sulfapehazole Derivatives as Tools for Comparing Cytochrome P4502C5 and Human Cytochromes P450 2CS: Identification of a New High Affinity Substrate Common to These Enzymes.
      Biochemistry 42, 6363-6369
Achieved using the OLIS DW-2

CH Yun, KH Kim, MW Calcutt, FP Guengerich. (2005). Kinetic Analysis of Oxidation of Coumarins by Human Cytochrome P450 2A6.
      J. Biological Chemistry 280, 12279-12291
Achieved using the OLIS DW2

S Herold, A Fago, RE Weber, S Dewilde, L Moens. (2004). Reactivity Studies of the Fe(III) and Fe(II)NO Forms of Human Neuroglobin Reveal a Potential Role against Oxidative Stress .
      J Biol Chem 279, 22841-22847
Achieved using the OLIS RSM 1000

AC Schoijet, K Miranda, W Girard-Dias, W de Sousa, MM Flwia, HN Torres, R Docampo, GD Alonso. (2008). A Trypanosoma cruzi Phosphatidylinositol 3-Kinase (TcVps34) Is Involved in Osmoregulation and Receptor-mediated Endocytosis .
      J Biol Chem 283, 31541-31550
Achieved using the OLIS DW2000

R Covian and BL Trumpower. (2009). The Rate-limiting Step in the Cytochrome bc1 Complex (Ubiquinol-Cytochrome c Oxidoreductase) Is Not Changed by Inhibition of Cytochrome b-dependent Deprotonation: IMPLICATIONS FOR THE MECHANISM OF UBIQUINOL OXIDATION AT CENTER P OF THE bc1 COMPLEX .
      J Biol Chem 284, 14359-14367
Achieved using the OLIS RSM 1000

MJ Longley, MM Humble, FS Sharief, WC Copeland. (2010). Disease Variants of the Human Mitochondrial DNA Helicase Encoded by C10orf2 Differentially Alter Protein Stability, Nucleotide Hydrolysis, and Helicase Activity.
      JBC 285, 29690-29702
Achieved using the OLIS RSM 1000

JC Low and SC Tu. (2002). Functional Roles of Conserved Residues in the Unstructured Loop of Vibrio harveyi Bacterial Luciferase.
      Biochemistry 41, 1724-1731
Achieved using the OLIS RSM 1000

M Castellani, R Covian, T Kleinschroth, O Anderkay, B Ludwig, BL Trumpower. (2010). Direct Demonstration of Half-of-the-sites Reactivity in the Dimeric Cytochrome bc1 Complex: ENZYME WITH ONE INACTIVE MONOMER IS FULLY ACTIVE BUT UNABLE TO ACTIVATE THE SECOND UBIQUINOL OXIDATION SITE IN RESPONSE TO LIGAND BINDING AT THE UBIQUINONE REDUCTION SITE .
      J Biol Chem 285, 502-510
Achieved using the OLIS RSM 1000

EB Gutierrez-Cirlos, T Merbitz-Zahradnik, BL Trumpower. (2004). Inhibition of the Yeast Cytochrome bc1 Complex by Ilicicolin H, a Novel Inhibitor That Acts at the Qn Site of the bc1 Complex.
      J Biol Chem 279, 8708-8714
Achieved using the OLIS DW2

YJ chun, S Kim, SK Lee, FP Guengerich. (2001). A New Selective and Potent Inhibitor of Human Cytochrome P450 1B1 and its Application to Antimutagenesis.
      Cancer Research 61, 8164-8170
Achieved using the OLIS DW2

M Hey-Mogensen, RLS Goncalves, AL Orr, MD Brand. (2014). Production of superoxide/H2O2?by dihydroorotate dehydrogenase in rat skeletal muscle mitochondria.
      Free Radical Biology and Medicine 72, 149-155
Achieved using the OLIS DW2

A Pena, NS Sanchez, M Calahorra. (2013). Effects of chitosan on Candida albicans. Conditions for its antifungal activity..
      Biomed Research International 527549
Achieved using the OLIS DW2

R Covian, EB Gutierrez-Cirlos, BL Trumpower. (2004). Anti-cooperative Oxidation of Ubiquinol by the Yeast Cytochrome bc1 Complex .
      J Biol Chem 279, 15040-15049
Achieved using the OLIS RSM 1000

C -H Tai, WM Rabeh, R Guan, KD Schnackerz, PF Cook. (2008). Role of Histidine-152 in cofactor orientation in the PLP-dependent O-acetylserine sulfhydrylase reaction.
      ABB 472, 115-125
Achieved using the OLIS RSM 1000F

Z Tang, SG Salamanca-Pinzon, Z -L Wu, Y Xiao, FP Guengerich. (2010). Human cytochrome P450 4F11: Heterologous expression in bacteria, purification, and characterization of catalytic function.
      ABB 494, 86-93
Achieved using the OLIS DW2

AA Narine, JN Watson, AJ Bennet. (2006). Mechanistic Requirements for the Efficient Enzyme-Catalyzed Hydrolysis of Thiosialosides.
      Biochemistry 45, 9319-9326
Achieved using the OLIS Cary 14

K Rahman, MAF Abdullah, S Ambati, MD Taylor, MJ Adang. (2012). Differential Protection of Cry1Fa Toxin against Spodoptera frugiperda Larval Gut Proteases by Cadherin Orthologs Correlates with Increased Synergism.
      Applied and Environmental Microbiology 78, 354-362
Achieved using the OLIS RSM 1000

SB Mulrooney, MJ Howard, RP Hausinger. (2011). The Escherichia coli alkylation response protein AidB is a redox partner of flavodoxin and binds RNA and acyl carrier protein.
      Arch. Biochem. Biophys. 513, 81-86
Achieved using the OLIS RSM 1000

VV Smirnov and JP Roth. (2014). Tyrosine oxidation in heme oxygenase: examination of long-range proton-coupled electron transfer.
      Journal of Biological Inorganic Chemistry 19, 1137-1148
Achieved using the OLIS RSM 1000

E Sforza, A Bertucco, T Morosinotto, GM Giacometti. (2011). Photobioreactors for microalgal growth and oil production with Nannochloropsis salina: From lab-scale experiments to large-scale design.
      Chem. Eng. Res. Design doi:10.1016/j.cherd.2011.12.002?
Achieved using the OLIS DM 45

KA Regal, ML Schrag, UM Kent, LC Wienkers, PF Hollenberg. (2000). Mechanism-Based Inactivation of Cytochrome P450 2B1 by 7-Ethynylcoumarin:? Verification of Apo-P450 Adduction by Electrospray Ion Trap Mass Spectrometry.
      Chem. Res. Toxicol. 13, 262-270
Achieved using the OLIS DW2

EM Isin, CD Sohl, RL Eoff, FP Guengerich. (2008). Cooperationof Cytochrome P450 1A2: Interactions of 1,4 Phenylene Diisocyanide and 1-Isoproxy-4-Nitrobenzene.
      Archives of Biochemistry and Biophysics 473, 69-75
Achieved using the OLIS DW2/Cary 14

AA Maddur, R Swanson, G Izaguirre, PGW Gettins, ST Olson. (2013). Kinetic intermediates en route to the final serpin-protease complex. Studies of complexes of ?1-protease inhibitor with trypsin.
      J Biol. Chem. 288, 32020-32035
Achieved using the OLIS SLM 8000

DA Mills, B Schmidt, C Hiser, E Whestley, S Furgeson-Miller. (2002). Membrane Potential-controlled Inhibition of Cytochromec Oxidase by Zinc .
      J Biol Chem 277, 14894-14901
Achieved using the OLIS RSM 1000

R Mukherjee, V Manivannan, ES Gould. (2007). Electron transfer. Part 165: Oxidations of Ti(II)(aq) with ligated iron(III) and ruthenium(III).
      Inorganica Chimica Acta 360, 3633-3636
Achieved using the OLIS Durram-Gibson

FA Al-Qalaf, AA Al Bassam, MM Shoukry. (2013). KINETICS AND MECHANISM OF BASE HYDROLYSIS OF A-AMINOACID ESTERS CATALYSED BY [Pd(1,3- DIAMINO-2-HYDROXYPROPANE)(H2O)2]2+ COMPLEX.
      Journal of the Chilean Chemical Society 58, 2
Achieved using the OLIS KinFit

S Goyal, Y Xiao, NA Porter, L Xu, FP Guengerich. (2014). Oxidation of 7-Dehydrocholesterol and Desmosterol by Human Cytochrome P450 46A1.
      Journal of Lipid Research 55, 1933-1943
Achieved using the OLIS DW 2

KS Lyle, JA Haas, BG Fox. (2003). Rapid-Mix and Chemical Quench Studies of Ferredoxin-Reduced Stearoyl-Acyl Carrier Protein Desaturase.
      Biochemistry 42, 5857-5866
Achieved using the OLIS RSM 1000F

H Park, G Kidman, DB Northrop. (2005). Effects of pressure on deuterium isotope effects of yeast alcohol dehydrogenase using alternative substrates.
      ABB 433, 335-340
Achieved using the OLIS DW2

MJ Ryle, A Liu, RB Muthukumaran, RYN Ho, KD Koehntop, J McCracken, L Que, Jr., RP Hausinger. (2002). O2- and ?-Ketoglutarate-Dependent Tyrosyl Radical Formation in TauD, an ?-Keto Acid-Dependent Non-Heme Iron Dioxygenase.
      Biochemistry 42, 1854-1862
Achieved using the OLIS RSM 1000

CS Farmer, DM Kurtz, RS Phillips, J Ai, J Sanders-Loehr. (2000). A Leucine Residue ?Gates? Solvent but Not O2Access to the Binding Pocket of Phascolopsis gouldiiHemerythrin .
      J Biol Chem 275, 17043-17050
Achieved using the OLIS RSM 1000

L Liu, DL Hachey, G Valadez, KM Williams, FP Guengerich, NA Loktionova, S Kanugula, AE Pegg. (2004). Characterization of a Mutagenic DNA Adduct Formed From 1,2-Dibromoethane by O6-alkylguanine-DNA Alkyltransferase.
      J. Biological Chemistry 279, 4250-4259
Achieved using the OLIS Cary 14

PM Pereira, M Teixeira, AV Xavier, RO Louro, IAC Pereira. (2006). The Tmc Complex from Desulfovibrio vulgaris Hildenborough Is Involved in Transmembrane Electron Transfer from Periplasmic Hydrogen Oxidation.
      Biochemistry 45, 10359-10367
Achieved using the OLIS DW2

I Forquer, MK Bowman, BL Trumpower, DM Kramer. (2006). Similar Transition States Mediate the Q-cycle and Superoxide Production by the Cytochrome bc1 Complex .
      J Biol Chem 281, 38459-38465
Achieved using the OLIS RSM 1000

T Shimada, RL Mernaugh, FP Guengerich. (2005). Interactions of mammalian cytochrome P450, NADPH-cytochrome P450 reductase, and cytochrome b5 enzymes.
      ABB 435, 207-216
Achieved using the OLIS DW2

F Zarate-Perez, ME Chanez-Carenas, R Arreola, A Torres-Larios, E Vasquez-Contreras. (2009). Different catalytic properties of two highly homologous triosephosphate isomerase monomers.
      Biochem. Biophys. Res. Comm. 382, 626
Achieved using the OLIS DM 45

MD Joshi, G Sidhu, JE Nielson, JD Brayer, SG Withers, LP McIntosh. (2001). Dissecting the Electrostatic Interactions and pH-Dependent Activity of a Family II Glycosidase.
      Biochemistry 40, 10115-10139
Achieved using the OLIS RSM 1000

N Cremades, A Velazquez-Campoy, M Martinez-Julvez, JL Neira, I Perez-Dorado, J Hermoso, P Jimenez, A Lanas, PS Hoffman, J Sancho. (2009). Discovery of Specific Flavodoxin Inhibitors as Potential Therapeutic Agents against Helicobacter pylori Infection.
      ACS Chem. Biol. 4, 928-938
Achieved using the OLIS Cary 14

S Mitroka, ME Shoman, JF DuMond, L Bellavia, OM Aly, M Abed-Aziz, DB Kim-Shapiro SB King. (2013). Direct and Nitroxyl (HNO)-Mediated Reactions of Acyloxy Nitroso Compounds with the Thiol-Containing Proteins Glyceraldehyde 3-Phosphate Dehydrogenase and Alkyl Hydroperoxide Reductase Subunit C.
      J. Medicinal Chemistry 56, 6583-6592
Achieved using the OLIS RSM 1000

DA Mills, L Geren, C Hiser, B Schmidt, B Durham, F Millet, S Ferguson-Miller. (2005). An Arginine to Lysine Mutation in the Vicinity of the Heme Propionates Affects the Redox Potentials of the Hemes and Associated Electron and Proton Transfer in Cytochrome c Oxidase.
      Biochemistry 44, 10457-10465
Achieved using the OLIS RSM 1000 SF

TI Doukov, H Hemmi, CL Drennan, SW Ragsdale. (2007). Structural and Kinetic Evidence for an Extended Hydrogen-bonding Network in Catalysis of Methyl Group Transfer: ROLE OF AN ACTIVE SITE ASPARAGINE RESIDUE IN ACTIVATION OF METHYL TRANSFER BY METHYLTRANSFERASES .
      J Biol Chem 282, 6609-6618
Achieved using the OLIS Cary 14

RS Phillips, HY Chen, D Shin, S Lima, K Tavakoli, B Sundararaju. (2004). Role of Lysine-256 in Citrobacter freundii Tyrosine Phenol-lyase in Monovalent Cation Activation.
      Biochemistry 43, 14412-14419
Achieved using the OLIS RSM 1000 SF

K Matsuura, S Yoshioka, S Takahashi, K Ishimor, T Mogi, h Hori, I Morishima. (2004). Dioxygen Reduction by bo-Type Quinol Oxidase from Escherichia coli Studied by Submillisecond Resolved Freeze-Quench EPR Spectroscopy.
      Biochemistry 43, 2288-2296
Achieved using the OLIS RSM 1000

H Yamazaki, T Shimada, MV Martin, FP Guengerich. (2001). Stimulation of Cytochrome P450 Reactions by Apo-cytochrome b5: Evidence Against Against Transfer of Heme from Cytochrome P450 3A4 to Apo-Cytochrome b5 or Heme Oxygenase.
      J. Biological Chemistry 276, 30885-30891
Achieved using the OLIS DW2/Cary 14

G Cerchiara, GA Micke, MFM Tavares, AM da Costa Fereira. (2004). Kinetic studies of carbohydrate oxidation catalyzed by novel isatin?Schiff base copper(II) complexes.
      J Molecular Catalysis A: Chemical 221, 29-39
Achieved using the OLIS DW 2000

JM Brewer, JS McKinnon, and RS Phillips. (2010). Stopped-flow studies of the reaction of d-tartronate semialdehyde-2-phosphate with human neuronal enolase and yeast enolase 1.
      FEBS Let 584, 979-983
Achieved using the OLIS RSM 1000

MZ Cabail, J Kostera, AA Pacheco. (2005). Laser Photoinitiated Nitrosylation of 3-Electron Reduced Nm europaea Hydroxylamine Oxidoreductase:? Kinetic and Thermodynamic Properties of the Nitrosylated Enzyme.
      Inorg. Chem. 44, 225-231
Achieved using the OLIS RSM flash system

TV Demidkina, NG Faleev, AI Papisova, NP Bazhulina, VV Kulikova, PD Gollnick, RS Phillips. (2006). Aspartic acid 214 in?Citrobacter freundii?tyrosine phenol-lyase ensures sufficient C?H-acidity of the external aldimine intermediate and proper orientation of the cofactor at the active site.
      BBA Proteins and Proteomics 1764, 1268-1276
Achieved using the OLIS RSM 1000

TK Nygaard, GC Blouin, M Liu, M Fukumura, JS Olson, M Fabian, DM dooley, B Lei. (2006). The Mechanism of Direct Heme Transfer from the Streptococcal Cell Surface Protein Shp to HtsA of the HtsABC Transporter .
      J Biol Chem 281, 20761-20771
Achieved using the OLIS RSM 1000

I Nogues, J Tejero, JK Hurley, D Paladini, S Frago, G Tollin, S Mayhew, C Gomez-Moreno, EA Ceccarelli, N Carrillo, M Medina. (2004). Role of the C-Terminal Tyrosine of Ferridoxin-Nicotinamide Adenine Dinucleotide Phosphate Reductase in the Electron Transfer Process with its Protein Partners Ferredoxin and Flavodoxin.
      Biochemistry 43, 6127-6137
Achieved using the OLIS GlobalWorks

JP Emerson, ED Coulter, DE Cabelli, RS Phillips, DM Kurtz Jr.. (2002). Kinetics and Mechanism of Superoxide Reduction by Two-Iron Superoxide Reductase from Desulfovibrio vulgaris.
      Biochemistry 41, 4348-4357
Achieved using the OLIS RSM 1000

GL Squadrito and WA Pryor. (2002). Mapping the Reaction of Peroxynitrite with CO2: Energetics, Reactive Species, and Biological Implications.
      Chem. Res. Toxic. 15, 885-895
Achieved using the OLIS RSM 1000

CL Quinlan, AA Gerencser, JR Treberg, MD Brand. (2011). The mechanism of superoxide production by the antimycin-inhibited mitochondrial Q-cycle.
      J Biol Chem 286, 31361-31372
Achieved using the OLIS DW2

MJ Cheeseman, MJ Traylor, ME Hilton, KE Richards, MC Taylor, PJ Daborn, RJ Russell, EMJ Gillam, JG Oakeshott. (2013). Soluble and membrane-bound?Drosophila melanogaster?CYP6G1 expressed in?Escherichia coli: Purification, activity, and binding properties toward multiple pesticides.
      Insect Biochemistry and Molecular Biology 43, 455-465
Achieved using the OLIS DW 2

H Park, GG Girdaukas, DB Northrop. (2006). Effect of Pressure on a Heavy-Atom Isotope Effect of Yeast Alcohol Dehydrogenase.
      JACS 128, 1868-1872
Achieved using the OLIS DW2

P Pospiail and A Tiwari. (2010). Differential mechanism of light-induced and oxygen-dependent restoration of the high-potential form of cytochrome b559 in Tris-treated Photosystem II membranes.
      Biochem. Biophys. Arch: Bioenergetics 1797, 451-456
Achieved using the OLIS RSM 1000F

RS Phillips, P McPhie, EW Miles, S Marachal, R Lange. (2008). Quantitative effects of allosteric ligands and mutations on conformational equilibria in Salmonella typhimurium tryptophan synthase.
      ABB 470, 8-19
Achieved using the OLIS Cary 14

K Xiao, L Yu, G-A Yu. (2000). Confirmation of the Involvement of Protein Domain Movement during the Catalytic Cycle of the Cytochrome bc 1Complex by the Formation of an Intersubunit Disulfide Bond between Cytochrome b and the Iron-Sulfur Protein .
      J Biol Chem 275, 38597-38604
Achieved using the OLIS RSM 1000

CD Sohl and FP Guengerich. (2010). Kinetic Analysis of the Three-step Steroid Aromatase Reaction of Human Cytochrome P450 19A1.
      JBC 285, 17734-17743
Achieved using the OLIS DW2/Cary 14/RSM 1000

KL Brown, U Bren, MP Stone, FP Guengerich. (2009). Inherent Stereospecificity in the Reaction of Aflatoxin B1 8,9-Epoxide with Deoxyguanosine and Efficiency of DNA Catalysis.
      Chemical Research and Toxicology 22, 913-917
Achieved using the OLIS Cary 14

AE McElhaney, FE Inscore, JT Schirlin, JH Enemark. (2002). Electron transfer studies of dithiolate complexes: effects of ligand variation and metal substitution.
      Inorganica Chimica Acta 341, 85-90
Achieved using the OLIS Cary 14

O Ecevit, MA Khan, DJ Goss. (2010). Kinetic Analysis of the Interaction of b/HLH/Z Transcription Factors Myc, Max, and Mad with Cognate DNA.
      Biochemistry 49, 2627-2635
Achieved using the OLIS RSM 1000F

DJ Quirk and DB Northrop. (2001). Effect of Pressure on Deuterium Isotope Effects of Formate Dehydrogenase.
      Biochemistry 40, 847-851
Achieved using the OLIS DW2

J Quin, H-L Fu, J Ye, KZ Bencze, TL Stemmler, DE Rawlings, BP Rosen. (2007). Convergent Evolution of a New Arsenic Binding Site in the ArsR/SmtB Family of Metalloregulators .
      J Biol Chem 282, 34346-34355
Achieved using the OLIS DSM 16

D Laraspata, V Gorgoglione, G La Piana, V Palmitessa, D Marzulli, NE Lofrumento. (2009). Interaction of nitric oxide with the activity of cytosolic NADH/cytochrome c electron transport system.
      ABB 489, 99-109
Achieved using the OLIS DW2000

C-A Chen, L Chi-Hung, JA Cowan. (2000). Functional role of a conserved tryptophan residue of?Chromatium vinosum?high potential iron protein.
      Inorganica Chimica Acta 300, 91-95
Achieved using the OLIS RSM 1000

RD McBane, C Gonzalez, DO Hodge, WE Wysok. (2013). Propensity for young reticulated platelet recruitment into arterial thrombi.
      Journal of Thrombosis and Thrombolysis 37, 148-154
Achieved using the OLIS SLM 8000

DA Mills and S Ferguson-Miller. (2002). Influence of structure, pH and membrane potential on proton movement in cytochrome oxidase.
      BBA Bioenergetics 1555, 96-100
Achieved using the OLIS RSM 1000

V Mathrubootham, J Thomas, R Staples, J McCraken, J Shearer, EL Hegg. (2010). Bisamidate and Mixed Amine/Amidate NiN2S2 Complexes as Models for Nickel-Containing Acetyl Coenzyme A Synthase and Superoxide Dismutase: An Experimental and Computational Study.
      Inorg. Chem 49, 5393-5406
Achieved using the OLIS Cary 14 - low temp

S Swavey and ES Gould. (2000). Electron Transfer. 140. Reactions of Riboflavin with Metal Center Reductants1.
      Inorg. Chem. 39, 352-256
Achieved using the OLIS Durrum SF

LM Hughes, R Covian, GW Gribble, BL Trumpower. (2010). Probing binding determinants in center P of the cytochrome bc1 complex using novel hydroxy-naphthoquinones.
      Biochem. Biophys Acta 1797, 38-43
Achieved using the OLIS RSM 1000

MS Afanasyen, MB Taraban, NE Polyakov, PA Purtov, TV Leshina, CB Grissom. (2006). Elementary Steps of Enzymatic Oxidation of Nifedipine Catalyzed by Horseradish Peroxidase.
      J Phys. Chem. B. 110, 21232-21237
Achieved using the OLIS RSM 1000 SF

J Hirst and DB Goodin. (2000). Unusual Oxidative Chemistry ofN ?-Hydroxyarginine and N-Hydroxyguanidine Catalyzed at an Engineered Cavity in a Heme Peroxidase .
      J Biol Chem 275, 8582-8591
Achieved using the OLIS RSM 1000

MK Gupta, OL Guryev, RJ Auchus. (2003). 5?-reduced C21 steroids are substrates for human cytochrome P450c17.
      ABB 418, 151-160
Achieved using the OLIS DW2

EB Gutierrez-Cirlos, T Merbitz-Zahradnik, BL Trumpower. (2002). Failure to Insert the Iron-Sulfur Cluster into the Rieske Iron-Sulfur Protein Impairs Both Center N and Center P of the Cytochrome bc 1 Complex .
      J Biol Chem 277, 50703-50709
Achieved using the OLIS RSM 1000

R Franco, JG Ma, Y Lu, GC Ferreira, JA Shelnutt. (2000). Porphyrin Interactions with Wild-Type and Mutant Mouse Ferrochelatase.
      Biochemistry 39, 2517-2529
Achieved using the OLIS RSM 1000F

R Shinkyo and FP Guengerich. (2010). Cytochrome P450 7A1 cholesterol 7?-hydroxylation. individual reaction steps in the catalytic cycle and rate-limiting ferric iron reduction.
      JBC 286, 4632-4643
Achieved using the OLIS DW2/Cary 14

PM Nielsen and A Fago. (2015). Inhibitory effects of nitrite on the reactions of bovine carbonic anhydrase II with CO2?and bicarbonate consistent with zinc-bound nitrite.
      Journal of Inorganic Biochemistry DOI:10.1016/j.jinorgbio.2015.04.013
Achieved using the OLIS RSM 1000

D Singh, D Gawal, M Itsko, A Hochkoeppler, JM Krahn, RE London, RM Schaaper. (2015). Structure of Escherichia coli dGTP Triphosphohydrolase: A Hexameric Enzyme With DNA Effector Molecules.
      Journal of Biological Chemistry 290, 10418-10429
Achieved using the OLIS RSM 1000

VL Davidson. (2003). Probing mechanisms of catalysis and electron transfer by methylamine dehydrogenase by site-directed mutagenesis of ?Phe55.
      BBA Proteins and Proteomics 1647, 230-233
Achieved using the OLIS RSM 1000

WE Karsten and PF Cook. (2002). Detection of intermediates in reactions catalyzed by PLP-dependent enzymes:?O-acetylserine sulfhydrylase and serine-glyoxalate aminotransferase.
      Methods in Enzymology 354, 223-237
Achieved using the OLIS RSM 1000

JK Tie, D-Y Jin, DR Louiselle, RM Pope, DL Straight, DW Stafford. (2004). Chemical Modification of Cysteine Residues Is a Misleading Indicator of Their Status as Active Site Residues in the Vitamin K-dependent ?-Glutamyl Carboxylation Reaction .
      J Biol Chem 279, 54079-54087
Achieved using the OLIS SLM 8000

FGM Wiertz, O-MH Richter, B Ludwig, S de Vries. (2007). Kinetic Resolution of a Tryptophan-radical Intermediate in the Reaction Cycle of Paracoccus denitrificans Cytochrome c Oxidase.
      J Biol Chem 282, 31580-31591
Achieved using the OLIS DW2000

AP Nigro and DB Goodin. (2010). Reaction of N-hydroxyguanidine with the ferrous?oxy state of a heme peroxidase cavity mutant: A model for the reactions of nitric oxide synthase.
      ABB 500, 66-73
Achieved using the OLIS RSM 1000

HK Joshi, JJA Cooney, FE Inscore, NE Gruhn, DL Lichtenberger, JH Enemark. (2003). Investigation of metal?dithiolate fold angle effects: Implications for molybdenum and tungsten enzymes .
      PNAS 100, 3719-3724
Achieved using the OLIS Cary 14

R Covian and BL Trumpower. (2008). The dimeric structure of the cytochrome?bc1?complex prevents center P inhibition by reverse reactions at center N.
      BBA Bioenergetics 1777, 1044-1052
Achieved using the OLIS RSM 1000

D Sun, X Li, FS Matthews, VL Davidson. (2005). Site Directed Mutagenesis of Proline 94 to Alanine in Amicyanin Converts a True Electron Transfer Reaction into One That is Kinetically Coupled.
      Biochemistry 44, 7200-7206
Achieved using the OLIS RSM 1000

FP Guengerich. (2005). Reduction of Cytochrome b5 by NADPH - Cytochrome P450 Reductase.
      Archives of Biochemistry and Biophysics 440, 204-211
Achieved using the OLIS Cary 14

J Seravalli, KL Brown, SW Ragsdale. (2001). Acetyl Coenzyme A Synthesis from Unnatural Methylated Corrinoids:? Requirement for ?Base-Off? Coordination at Cobalt.
      JACS 123, 1786-1787
Achieved using the OLIS Cary 14

AT Russo, J Rosgen, DW Bolen. (2003). Osmolyte Effects on Kinetics of FKBP12 C22A Folding Coupled with Prolyl Isomerization.
      J Mol Biol 330, 851-866
Achieved using the OLIS ?????

K -H Tang, AD Casarez, W Wu, PA Frey. (2003). Kinetic and biochemical analysis of the mechanism of action of lysine 5,6-aminomutase.
      ABB 418, 49-54
Achieved using the OLIS RSM 1000

FP Guengerich, GP Miller, IH Hanna, H Sato, MV Martin. (2002). Oxidations of Methoxyphenethylamines by Cytochrome P450 2D6: Analysis of Rate Limiting Steps.
      J. Biological Chemistry 277, 33711-33719
Achieved using the OLIS Cary 14

JA Krauser and FP Guengerich. (2005). Cytochrome P450 3A4 - Catalyzed Testosterone 6-(beta)-Hydroxylation Stereochemistry, Kinetic Dueterium Isotope Effects and Rate-limiting Steps.
      J. Biological Chemistry 280, 19496-19506
Achieved using the OLIS Cary 14

Y Liu and BC Hill. (2007). Formamide probes a role for water in the catalytic cycle of cytochrome?c?oxidase.
      BBA Bioenergetics 1767, 45-55
Achieved using the OLIS RSM 1000

IH Hanna, S. Dawling, N Roodi, FP Guengerich. (2000). Cytochrome P450 1B1 (CTP 1B1) Pharmacogenetics Association of Polymophisms with functional Differences in Estrogen Hydroxylation Activity.
      Cancer Research 60, 3440-3444
Achieved using the OLIS DW2

AJ Cruz, K Siam, C Moore, M Islam, and DP Rillema. (2010). Dicyano and Pyridine Derivatives of Retinal: Synthesis and Vibronic, Electronic, and Photophysical Properties.
      J Phys Chem A 114, 6766-6775
Achieved using the OLIS RSM 1000

ZL Wu, CJ Bartleson, AJL Ham, FP Guengerich. (2006). Heterologous Expression, Purification, and Properties of Human Cytochrome P450 27C1.
      Archives of Biochemistry and Biophysics 445, 138,146
Achieved using the OLIS DW2

EM Maes, A Weichsel, JF Anderson, D Shepley, WR Monfort. (2004). Role of Binding Site Loops in Controlling Nitric Oxide Release:? Structure and Kinetics of Mutant Forms of Nitrophorin 4,.
      Biochemistry 43, 6679-6690
Achieved using the OLIS RSM 1000 SF

FP Guengerich, IH Hanna, MV Martin, EMJ Gillan. (2003). Role of Glutamic Acid 216 in Cytochrome P4502D6 Substrate Binding and Catalysis.
      Biochemistry 42, 1245-1253
Achieved using the OLIS Cary 14

B Sundararaju, H Chen, S Shilcutt, RS Phillips. (2000). The Role of Glutamic Acid-69 in the Activation of Citrobacter freundii Tyrosine Phenol-Lyase by Monovalent Cations.
      Biochemistry 39, 8546-8555
Achieved using the OLIS RSM 1000 SF

S Lee, S Shin, X Li, VL Davidson. (2009). Kinetic Mechanism for the Initial Steps in MauG-Dependent Tryptophan Tryptophylquinone Biosynthesis.
      Biochemistry 48, 2442-2447
Achieved using the OLIS RSM 1000 SF

H Zhang, U Bren, ID Kozekov, CJ Rizzo, DF Stec, FP Guengerich. (2009). Steric and Electrostatic Effects at the C2 Atom Substituent Influence Replication and Miscoding of the DNA Deamination Product Deoxyxanthosine and Analogs by DNA Polymerases?.
      J Mol. Biol. 392, 251
Achieved using the OLIS DW2

KM Cunningham and RE McCarty. (2000). Influences of Energinization and Nucleotide Binding on the Reaction of Lucifer Yellow Vinyl Sulfone with the alpha-subunits of the Chloroplast ATP Synthase.
      Biochemistry 39, 4391-4398
Achieved using the OLIS SLM SPF-500

BG Fritz, SA Roberts, A Ahmed, L Breci, W Li, A Weichsel, JL Brailey, VH Wysocki, F Tama, WR Montfort. (2013). Molecular Model of a Soluble Guanylyl Cyclase Fragment Determined by Small-Angle X-ray Scattering and Chemical Cross-Linking.
      Biochemistry 52, 1568-1582
Achieved using the OLIS RSM 1000

JR Reed, M Eyer, WL Backes. (2010). Functional Interactions between Cytochromes P450 1A2 and 2B4 Require Both Enzymes to Reside in the Same Phospholipid Vesicle: EVIDENCE FOR PHYSICAL COMPLEX FORMATION .
      JBC 285, 8942-8952
Achieved using the OLIS RSM 1000

BM Rock, SM Hengel, DA Rock, LC Wienkers, KL Kunze. (2014). Characterization of Ritonavir-Mediated Inactivation of Cytochrome P450 3A4.
      Molecular Pharmacology 86, 665-674
Achieved using the OLIS DW 2

S -H Cho and FP Guengerich. (2011). Conjugation of Butadiene Diepoxide with Glutathione Yields DNA Adducts In Vitro and In Vivo.
      Chem. Res. Toxicol. 25, 706-712
Achieved using the OLIS Cary 14

GP Miller, IH Hanna, Y Nishimura, FP Guengerich. (2001). Oxidation of Phenethylamine Derivatives by Cytochrome P450 2D6: The Issue of Substrate Protonation in binding and Catalysis.
      Biochemistry 40, 14215-14223
Achieved using the OLIS DW2

LJ Bailey and BG Fox. (2009). Crystallographic and Catalytic Studies of the Peroxide-Shunt Reaction in a Diiron Hydroxylase.
      Biochemistry 48, 8932-8939
Achieved using the OLIS RSM 1000

P Ryan and MJ Hynes. (2007). The kinetics and mechanisms of the complex formation and antioxidant behaviour of the polyphenols EGCg and ECG with iron(III).
      J Inorg Biochem 101, 585-593
Achieved using the OLIS Kinfit

J Xiong, RS Phillips, DM Kurtz, Jr., S Jin, J ai, J Sanders-Loehr. (2000). The O2 Binding Pocket of Myohemerythrin:? Role of a Conserved Leucine.
      Biochemistry 39, 8526-8536
Achieved using the OLIS RSM 1000 SF

P Rohloff, K Miranda, JCF Rodrigues, J Fang, M Galizzi, H Plattner, J Hentschel, SNJ Moreno. (2011). Calcium Uptake and Proton Transport by Acidocalcisomes ofToxoplasma gondii.
      PLoS ONE 6(4): e18390
Achieved using the OLIS DW2000

AB Mason, PJ Halbrooks, NG James, SA Connolly, JR Larouche, VC Smith, RTA MaGillivray, ND Chasteen. (2005). Mutational Analysis of C-Lobe Ligands of Human Serum Transferrin:? Insights into the Mechanism of Iron Release.
      Biochemistry 44, 8013-8021
Achieved using the OLIS RSM 1000 SF

RG Leon, H Munier-Lehman, O Barzu, V Baudin-Creuza, R Pietri, J Lopez-Garriga, CL Cadilla. (2004). High-level production of recombinant sulfide-reactive hemoglobin I from?Lucina pectinata?in?Escherichia coli: High yields of fully functional holoprotein synthesis in the BLi5?E. coli?strain.
      Protein Expression and Purification 38, 184-195
Achieved using the OLIS RSM 1000

WD Lu and WM Atkins. (2004). A Novel Antioxidant Role for Ligand in Behavior of Glutathione S-Transferases: Attenuation of the Photodynamic Effects of Hypericin.
      Biochemistry 43, 12761-12769
Achieved using the OLIS DW-2

R Kaspara, SB Naraharisetti, EA Evangelista, KC Marciante, BM Psaty, RA Totah. (2011). Drug metabolism by CYP2C8.3 is determined by substrate dependent interactions with cytochrome P450 reductase and cytochrome b5.
      Biochemical Pharmacology 82, 681-691
Achieved using the OLIS DW2

CL Quinlan, RLS Goncalves, M Hey-Mogensen, N Yadava, VI Bunik, MD Brand. (2014). The 2-Oxoacid Dehydrogenase Complexes in Mitochondria Can Produce Superoxide/Hydrogen Peroxide at Much Higher Rates than Complex I.
      Journal of Biological Chemistry 289, 8312-8325
Achieved using the OLIS DW 2

T Tosha, S Yoshioka, H Hori, S Takahashi, K Ishimori, I Morishima. (2002). Molecular Mechanism of the Electron Transfer Reaction in Cytochrome P450cam?Putidaredoxin:? Roles of Glutamine 360 at the Heme Proximal Site.
      Biochemistry 41, 13883-13893
Achieved using the OLIS RSM 1000 SF

MT Honaker, M Acchione, W Zhang, B Mannervik, WM Atkins. (2013). Enzymatic Detoxification, Conformational Selection, and the Role of Molten Globule Active Sites.
      J. Biol. Chem. 288, 18599-18611
Achieved using the OLIS DW 2

SP Wu and JA Cowan. (2003). Iron-Sulfur Cluster Biosynthesis. A Comparative Kinetic Analysis of Native and Cys-Substituted ISA-Mediated [2Fe-2S]2+ Cluster Transfer to an Apoferredoxin Target.
      Biochemistry 42, 5784-5791
Achieved using the OLIS RSM and HPDA

T Shimada, S Takenaka, N Murayama, H Yamazaki, J-H Kim, D Kim, FK Yoshimoto, FP Guengerich, M Komori. (2015). Oxidation of Acenaphthene and Acenaphthylene by Human Cytochrome P450 Enzymes.
      Chemical Research in Toxicology 28, 268-278
Achieved using the OLIS DW 2

JM Hutzler, RJ Melton, JM Rumsey, ME Schnute, CW Loucuson, LC Wiekers. (2006). Inhibition of Cytochrome P450 3A4 by a Pyrimidineimidazole:? Evidence for Complex Heme Interactions.
      Chem. Res. Toxicol. 19, 1650-1659
Achieved using the OLIS DW2000

T Lendrihas, GA Hunter, GC Ferreira. (2010). Targeting the Active Site Gate to Yield Hyperactive Variants of 5-Aminolevulinate Synthase.
      JBC 285, 13704-13711
Achieved using the OLIS RSM 1000F

PS Pallan, LD Nagy, L Lei, E Gonzalez, VM Kamlinger, CM Azumaya, Z Wawrzak, MR Waterman, FP Guengerich, M Egli. (2015). Structural and Kinetic Basis of Steroid 17?,20-Lyase Activity in Teleost Fish Cytochrome P450 17A1 and Its Absence in Cytochrome P450 17A2.
      J. Biological Chemistry 290, 3248-3268
Achieved using the OLIS DW 2

S Guo, GZ Ferl, R Deora, M Riedinger, S Yin, JL Kerwin, JA Loo, ON Witte. (2004). A phosphorylation site in Bruton's tyrosine kinase selectively regulates B cell calcium signaling efficiency by altering phospholipase C-? activation .
      PNAS 101, 14180-14185
Achieved using the OLIS SLM 8000

A Franke, E Hartman, I Schlichting, R van Eldik. (2012). A complete volume profile for the reversible binding of camphor to cytochrome P450cam.
      J. Biol. Inorg. Chem. 17, 447-463
Achieved using the OLIS KinFit

E Murakami, U Deppenmeir, SW Ragsdale. (2001). Characterization of the Intramolecular Electron Transfer Pathway form 2-Hydroxyphenazine to the Heterodisulfide Reductase from Methanosarcina thermophila.
      JBC 276, 2432
Achieved using the OLIS Cary 14

M Fabian, E Solomaha, JS Olson, AW Maresso. (2009). Heme Transfer to the Bacterial Cell Envelope Occurs via a Secreted Hemophore in the Gram-positive Pathogen Bacillus anthracis .
      J Biol Chem 284, 32138-32146
Achieved using the OLIS RSM 1000

X Hu, LB Murata, A Weichsel, JL Brailey, SA Roberts, A Nighorn, WR Montfort. (2008). Allostery in Recombinant Soluble Guanylyl Cyclase from Manduca sexta .
      J Biol Chem 283, 20968-20977
Achieved using the OLIS RSM 1000

C Feng, G Tollin, MA Holliday, C Thomas, JC Salerno, JH Enemark, DK Ghosh. (2006). Intraprotein Electron Transfer in a Two-Domain Construct of Neuronal Nitric Oxide Synthase:? The Output State in Nitric Oxide Formation.
      Biochemistry 45, 6354-6362
Achieved using the OLIS DW2000

EB Watkins and RS Phillips. (2001). Inhibition of Tyrosine Phenol-lyase from Citrobacter freundii by 2-Azatyrosine and 3-Azatyrosine.
      Biochemistry 40, 14862-14868
Achieved using the OLIS RSM 1000 SF

VL Davidson, LH Jones, ME Graichen, Z Zhu. (2000). Tyr30?of amicyanin is not critical for electron transfer to cytochrome?c-551i: implications for predicting electron transfer pathways.
      BBA Bioenergetics 1457, 27-35
Achieved using the OLIS RSM 1000

J Liu, TT Nguyen, PS Dupart, J Sridhar, X Zhang, N Zhu, CK Stevens, M Foroozesh. (2012). 7-Ethynylcoumarins: Selective Inhibitors of Human Cytochrome P450s 1A1 and 1A2.
      Chem. Res. Toxicol. 25, 1047-1057
Achieved using the OLIS DM 45

MZ Cabail and AA Pacheco. (2003). Selective One-Electron Reduction of Nitrosomonas europaea Hydroxylamine Oxidoreductase with Nitric Oxide.
      Inorg. Chem. 42, 270-272
Achieved using the OLIS RSM 1000

PD Gollnick and RS Phillips. (2003). Role of Aspartate-133 and Histidine-458 in the Mechanism of Tryptophan Indole-Lyase from Proteus vulgaris.
      Biochemistry 42, 11161-11169
Achieved using the OLIS RSM 1000 SF

I Azarov, KT Huang, S Basu, MT Gladwin, N Hogg, DB Kim-Shapiro. (2005). Nitric Oxide Scavenging by Red Blood Cells as a Function of Hematocrit and Oxygenation .
      J Biol Chem 280, 39024-39032
Achieved using the OLIS RSM 1000

L Zhao, MG Pence, RL Eoff, S Yuan, CA Fercu, FP Guengerich. (2014). Elucidation of Kinetic Mechanisms of Human Translesion DNA Polymerase ? Using Tryptophan Mutants.
      FEBS Journal 281, 4394-4410
Achieved using the OLIS Cary 14

GK Freschauf, RS Mani, TR Mereniuk, M Fanta, CA Virgen, GL Dianov, J-M Grassot, DG Hall, M Weinfeld. (2010). Mechanism of Action of an Imidopiperidine Inhibitor of Human Polynucleotide Kinase/Phosphatase .
      JBC 285, 2351-2360
Achieved using the OLIS DSM 17

B Gerratana, WW Cleland, PA Frey. (2001). Mechanistic Roles of Thr134, Tyr160, and Lys164 in the Reaction Catalyzed by dTDP-Glucose-4,6-Dehydrogenase.
      Biochemistry 40, 9187-9195
Achieved using the OLIS RSM 1000

BT Farrer and VL Pecoraro. (2003). Hg(II) binding to a weakly associated coiled coil nucleates an encoded metalloprotein fold: A kinetic analysis .
      PNAS 100, 3760-3765
Achieved using the OLIS RSM 1000

RS Phillips, B Sundararaju, N Faleev. (2000). Proton Transfer and Carbon?Carbon Bond Cleavage in the Elimination of Indole Catalyzed by Escherichia coli Tryptophan Indole-Lyase.
      JACS 122, 1008-1014
Achieved using the OLIS RSM 1000 SF

EB Gutierrez-Cirlos and BL Trumpower. (2002). Inhibitory Analogs of Ubiquinol Act Anti-cooperatively on the Yeast Cytochrome bc 1 Complex: EVIDENCE FOR AN ALTERNATING, HALF-OF-THE-SITES MECHANISM OF UBIQUINOL OXIDATION .
      J Biol Chem 277, 1195-1202
Achieved using the OLIS DW2

Y Xiao and FP Guengerich. (2012). Metabolomic analysis and identification of a role for the orphan human cytochrome P450 2W1 in selective oxidation of lysophospholipids.
      J. Lipid Res. 53, 1610-1617
Achieved using the OLIS DW2

C-H Tai, WM Rabeh, R Guan, KD Schnacker, PF Cook. (2008). Role of Histidine-152 in cofactor orientation in the PLP-dependent?O-acetylserine sulfhydrylase reaction.
      ABB 472, 115-125
Achieved using the OLIS RSM 1000

RS Mani, Y Yu, S Fang, M Lu, M Fanta, AE Zolner, N Tahbaz, DA Ramsden, DW Litchfield, SP Lees-Miller, M Weinfield. (2010). Dual Modes of Interaction between XRCC4 and Polynucleotide Kinase/Phosphatase IMPLICATIONS FOR NONHOMOLOGOUS END JOINING*.
      JBC 285, 37619
Achieved using the OLIS RSM 1000

DA Mills, Z Tan, S Ferguson-Miller, J Hosler. (2003). A Role for Subunit III in Proton Uptake into the D Pathway and a Possible Proton Exit Pathway in Rhodobacter sphaeroides Cytochrome c Oxidase.
      Biochemistry 42, 7410-7417
Achieved using the OLIS RSM 1000 SF

J Qian, DA Mills, L Green, K Wang, CW Hoganson, B Schmidt, C Hiser, GT Babcock, B Durham, F Millett, S Ferguson-Miller. (2004). Role of the Conserved Arginine Pair in Proton and Electron Transfer in Cytochrome c Oxidase.
      Biochemistry 43, 5748-5756
Achieved using the OLIS RSM 1000 SF

W Zhang, JS Olson, GN Phillips, Jr.. (2005). Biophysical and Kinetic Characterization of HemAT, an Aerotaxis Receptor from Bacillus subtilis .
      Biophys J 88, 2801-2814
Achieved using the OLIS Gibson-Dionex

Client Publications