Protein Secondary Structure

Protein secondary structure is an important consideration when studying protein folding, protein stability, or enzyme activity.

A powerful tool used to monitor secondary structure is circular dichroism (CD), which is the differential absorbance of left and right circularly polarized light. Since proteins consist of chiral amino acids, they are CD active and exhibit distinct CD spectra that are sensitive to the protein’s secondary structure. This spectra can provide a qualitative snapshot of the protein structure or the secondary structure composition of a protein can be estimated from the far UV CD spectrum using a number of algorithms, several of which are made available in Olis GlobalWorks software.

Protein secondary structure can also be used to monitor protein folding and unfolding. An equilibrium study consists of collection of CD spectra as a function of increasing temperature or chemical denaturant. As the protein unfolds, the CD spectrum reflects this. Fitting data at a single wavelength or the full spectral scans during this denaturation process provides thermodynamic information such as the melting temperature and the enthalpy of unfolding. Both these parameters provide important information about the stability of the protein under study.

The DSM 17, DSM 20, DSM 1000, and the Multiscan support protein secondary structural studies. Accessories such as the CD 250 Peltier cell holder provide a convenient method for conducting these experiments.

Stopped Flow

The kinetics of protein folding and unfolding can be monitored by the use of a stopped flow accessory. Millisecond changes to protein structure can be studied by observing the CD signal at an informative wavelength during the course of a stopped flow reaction. GlobalWorks also supports the kinetic analysis of these kinetic traces.

Looking for Paul's Practical Guide to CD?

Visit the Literature gallery and look for Paul’s Practical Guide to CD under the Manuals section.

D Meinhold, M Beach, Y Shao, R Osuna, W Colon. (2006). The Location of an Engineered Inter-Subunit Disulfide Bond in Factor for Inversion Stimulation (FIS) Affects the Denaturation Pathway and Coopertivity.
      Biochemistry 45, 9767-9777
Achieved using the OLIS CD????

SY Lo, CE Sabel, JP Mapletoft, S Siemann. (2015). Influence of chemical denaturants on the activity, fold and zinc status of anthrax lethal factor.
      Biochemistry and Biophysics 1, 68-77
Achieved using the OLIS RSM 1000

S Lu, M Liu, B Ni. (2011). Degradable, injectable poly(N-isopropylacrylamide)-based hydrogels with low gelation concentrations for protein delivery application.
      Chemical Engineering Journal 173, 241-250
Achieved using the OLIS DSM 1000

NE Kurland, T Dey, SC Kundu, VK Yadavalli. (2013). Precise Patterning of Silk Microstructures Using Photolithography.
      Advanced Materials 25, 6207-6212
Achieved using the OLIS DSM 1000

AF Mehl, K Okada, SM Dehn, S Kurian. (2012). Probing the Stability and Mechanism for Folding of the GrpE1-112 Tetrameric Deletion Mutant of the GrpE Protein from E. coli.
      Biochem. Biophys Res. Comm. 420, 635-638
Achieved using the OLIS DSM 1000 +SF

Z Li, D Roccatano, M Lorenz, R Martinez, U Schwaneberg. (2013). Insights on activity and stability of subtilisin E towards guanidinium chloride and sodium dodecylsulfate.
      Journal of Biotechnology 169C, 87-94
Achieved using the OLIS DSM 17

AG Krishna, ST Menon, TJ Terry, TP Sakmar. (2002). Evidence That Helix 8 of Rhodopsin Acts as a Membrane-Dependent Conformational Switch.
      Biochemistry 41, 8298-8309
Achieved using the OLIS DSM 1000

MR Larson, KR Rajashankar, MH Patel, RA Robinette, PJ Crowley, S Michalek, LJ Brady, C Deinvanayagan. (2010). Elongated fibrillar structure of a streptococcal adhesin assembled by the high-affinity association of ?- and PPII-helices.
      PNAS 107, 5983-5988
Achieved using the OLIS DSM 1000

H-Z He, M Wang, DS-H Chan, C-H Leung, J-W Qiu, D-L Ma. (2013). A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine.
      Methods 64, 205-211
Achieved using the OLIS DSM 1000?

D -L Zhang, D Su, A Berczi, A Vargus, H Asard. (2006). An ascorbate-reducible cytochrome b561 is localized in macrophage lysosomes.
      BBA General Subjects 1760, 1903-1913
Achieved using the OLIS DW2000

RM Alvey, A Biswas, WM Schluchter, DA Bryant. (2011). Attachment of Non-cognate Chromophores to CpcA of Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002 by Heterologous Expression in Escherichia coli.
      Biochemistry 50, 4890-4902
Achieved using the OLIS SLM 8000

S Chen, V Berelier, W Yang, R Wetzel. (2001). Polyglutamine aggregation behavior?in vitro?supports a recruitment mechanism of cytotoxicity.
      J Molecular Biology 311, 173-182
Achieved using the OLIS DSM 1000

S Boswell, J Matthew, M Beach, R Osuna, W Colon. (2004). Variable Contributions of Tyrosine Residues to the Structural and Spectroscopic Properties of the Factor for Inversion Stimulation.
      Biochemistry 43, 2964-2977
Achieved using the OLIS DSM 10

W Shang, JH Nuffer, JS Dordick, RW Siegel. (2007). Unfolding of Ribonuclease A on Silica Nanoparticle Surfaces.
      Nano Lett 7, 1991-1995
Achieved using the OLIS DSM 10

A Chattopadhyay, M Meier, S Ivaniskii, P Burkhard, F Speroni, B Camanin, S Bettata, A Mozzarelli, WM Rabeh, L Li, PF Cook. (2007). Structure, Mechanism, and Conformational Dynamics of O-Acetylserine Sulfhydrylase from Salmonella typhimurium:? Comparison of A and B Isozymes.
      Biochemistry 46, 8315-8330
Achieved using the OLIS RSM 1000 SF

KW Bender, S Dobney, A Ogunrinde, D Chiasson, RT Mullen, HJ Teresinski, P Singh, K Munro, SP Smith, WA Snedden. (2014). The calmodulin-like protein, CML43, functions as a salicylic acid-inducible, root-specific Ca2+ sensor in Arabidopsis.
      Biochemical Journal 457, 127-136
Achieved using the OLIS DSM 1000

B Wheeler and Z Jia. (2014). Preparation and characterization of human ADCK3, a putative atypical kinase.
      Protein Expression and Purification 108C, 13-17
Achieved using the OLIS DSM 1000

J Bai, DJ Swartz, II Protasevich, CG Brouillette, PM Harrell, E Hildebrandt, B Gasser, D Mattanovich, A Ward, C Chang, IL Urbatsch. (2011). A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris.
      PLoS ONE 6(8): e22577
Achieved using the OLIS DSM 1000

H Etayash, LL Norman, T Thundat, K Kaur. (2013). Peptide-Bacteria Interactions using Engineered Surface Immobilized Peptides from Class IIa Bacteriocins.
      Langmuir 29, 4048-4056
Achieved using the OLIS DSM 17

TM Josephs, IM Morison, CL Day, SM Wilbanks, EC Ledgerwood. (2014). Enhancing the peroxidase activity of cytochrome c by mutation of residue 41: implications for the peroxidase mechanism and cytochrome c release.
      Biochemical Journal 458, 259-265
Achieved using the OLIS DSM 10

E Longo, E De Santis, R Hussain, CF van der Walle, J Casa-Finet, S Uddin, A Dos Santos, G Siligardi. (2014). The effect of palmitoylation on the conformation and physical stability of a model peptide hormone.
      International Journal of Pharmaceutics 472, 156-164
Achieved using the OLIS GlobalWorks

X Wang, X Xie, C Ren, Y Yang, X Xu, X Chen. (2010). Application of molecular modeling and spectroscopic approaches for investigating binding of vanillin to human serum albumin.
      Food Chemistry 127, 705-710
Achieved using the OLIS DSM 1000

NB Gillespie, KJ Wise, L Ren, JA Stuart, DL Marcy, Hillebrecht, Q Li, L Ramos, K Jordan, S Fyvie, RR Birge. (2002). Characterization of the Branched-Photocycle Intermediates P and Q of Bacteriorhodopsin.
      J. Phys. Chem. B 106, 13352-13361
Achieved using the OLIS RSM 1000

K Wang, J Yan, J Zhang, R Chan, B Zhang, W Dang, W Zhang, M Kai, J Song, R Wang. (2011). Novel Cytotoxity Exhibition Mode of Polybia-CP, a Novel Antimicrobial Peptide from the Venom of the Social Wasp Polybia paulista.
      Toxicology 288, 27-33
Achieved using the OLIS DSM 1000

CM Mosher, MA Hummel, TS Tracy, AE Rettie. (2008). Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9.
      Biochemistry 47, 11725-11734
Achieved using the OLIS DW2

S Raccosta, V Martorana, M Manno. (2012). Thermodynamic Versus Conformational Metastability in Fibril-Forming Lysozyme Solutions.
      J. Phys. Chem. B 116, 12078-12087
Achieved using the OLIS DSM 10

MM Pereira, K Jones, MG Campos, AMP Melo, LM Saraiva, RO Loura, P Wittung-Stafshede, M Teixera. (2003). A ferredoxin from the thermohalophilic bacterium?Rhodothermus marinus.
      BBA Proteins and Proteomics 1601, 1-8
Achieved using the OLIS DSM 1000

MG Sehorn, SV Slepenkov, SN Witt. (2002). Characterization of Two Partially Unfolded Intermediates of the Molecular Chaperone DnaK at Low pH.
      Biochemistry 41, 8499-8507
Achieved using the OLIS DSM 16

K Tang, M Knipp, B-B Liu, N Cox, R Stabel Q He, M Zhou, H Scheer, K-H Zhao, W Gaertner. (2015). Redox-dependent ligand switching in a sensory heme-binding gaf domain of the cyanobacterium NOSTOC SP. PCC7120.
      Journal of Biological Chemistry DOI: 10.1074/jbc.M115.654087
Achieved using the OLIS 1.4 T Magnet (with Jasco)

K Lecours, M-H Tremblay, M-EL Gagne, SM Gagne, D LeClerc. (2006). Purification and biochemical characterization of a monomeric form of papaya mosaic potexvirus coat protein.
      Protein Expression and Purification 47, 273-280
Achieved using the OLIS RSM 1000

NE Kurland, J Kundu, S Pal, SC Kundu, VK Yadavalli. (2012). Self-assembly mechanisms of silk protein nanostructures on two-dimensional surfaces.
      Soft Matter 4952-4959
Achieved using the OLIS DSM 1000

M Choi, N Sukumar, A Liu, VL Davidson. (2009). Defining the Role of the Axial Ligand of the Type 1 Copper Site in Amicyanin by Replacement of Methionine with Leucine.
      Biochemistry 48, 9174-9184
Achieved using the OLIS RSM 1000

JR Kramer, AR Rodriguez, U-J Choe, DT Kanei, TJ Deming. (2013). Glycopolypeptide conformations in bioactive block copolymer assemblies influence their nanoscale morphology.
      Soft Matter 9, 3389-3395
Achieved using the OLIS DSM 1000

JN Lampe, R Brandman, S Sivaramakrishnan, PR Ortiz de Montellano. (2010). Two-dimensional NMR and All-atom Molecular Dynamics of Cytochrome P450 CYP119 Reveal Hidden Conformational Substates .
      JBC 285, 9594-9603
Achieved using the OLIS DW2

KC Bodapati, R Soudy, H Etayash, M Stiles, K Kaur. (2013). Design, synthesis and evaluation of antimicrobial activity of N-terminal modified Leucocin A analogues.
      Bioorganic and Medicinal Chemistry 21, 3715-3722
Achieved using the OLIS DSM 17

L Wang, HA Lashuel, T Walz, W Colon. (2002). Murine apolipoprotein serum amyloid A in solution forms a hexamer containing a central channel .
      PNAS 99, 15947-15952
Achieved using the OLIS DSM CD

J Maia, H Vazao, DCS Pedroso, CSH Jesus, RMM Brito, M Graos, MH Gil, LS Ferreira. (2012). VEGF-functionalized dextran has longer intracellular bioactivity than VEGF in endothelial cells.
      BioMacromolecules 13, 2906-2916
Achieved using the OLIS DSM 20

MP Bronnimann, JA Chapman, CK Park, SK Campos. (2012). A Transmembrane Domain and GxxxG Motifs Within L2 are Essential for Papillomavirus Infection..
      J. Virology 87, 464-473
Achieved using the OLIS DSM 20

D Meinhold, S Boswell, W Colon. (2005). P61A Mutation in the Factor for Inversion Stimulation Results in a Thermostable Dimeric Intermediate.
      Biochemistry 44, 14715-14724
Achieved using the OLIS DSM 10

PF Minimol, W Paul, and CP Sharma. (2013). PEGylated Starch Acetate Nanoparticles and its Potential Use for Oral insulin Delivery.
      Carbohydrate Polymers 95, 1-8
Achieved using the OLIS GlobalWorks

L Zhu, F Cheng, V Piatkowski, U Schwaneberg. (2013). Protein Engineering of the Antitumor Enzyme PpADI for Improved Thermal Resistance.
      ChemBioChem DOI:?10.1002/cbic.201300433
Achieved using the OLIS DSM 17

B Jeppesen, L Costello, A fung, E Stanley, J McDonald, A Lambert, B Johnson, L Gentile. (2007). Structure nor stability of the transmembrane spanning 6/7 domain of presenilin I correlates with pathogenicity.
      BBRC 355, 820-824
Achieved using the OLIS DSM 10

MC De Jesus, BI Ingle, KA Barakat, B Sherstha, KD Slavens, TR Cundari, ME Anderson. (2014). The Role of Strong Electrostatic Interactions at the Dimer Interface of Human Glutathione Synthetase.
      The Protein Journal 33, 403-9
Achieved using the OLIS DSM 1000

H Popelkova, A Commet, CF Yocum. (2010). The double mutation ?L6MW241F in PsbO, the photosystem II manganese stabilizing protein, yields insights into the evolution of its structure and function.
      FEBS Lett. 584, 4009
Achieved using the OLIS Cary 17

C Bleiholder, TD Do, C Wu, NJ Economou, SS Bernstein, SK Buratto, J-E Shea, MT Bowers. (2013). Ion mobility spectrometry reveals the mechanism of amyloid formation of A?(25-35) and its modulation by inhibitors at the molecular level: Epigallocatechin gallate and scyllo-inositol..
      Journal of the American Chemical Society 135, 16926-16937
Achieved using the OLIS DSM 1000

MK Sharma and ML Gilchrist. (2007). Templated Assembly of Biomembranes on Silica Microspheres Using Bacteriorhodopsin Conjugates as Structural Anchors.
      Langmuir 23, 7101-7112
Achieved using the OLIS DSM 20

JF Andersen, XD Ding, C Balfour, TK Shokireva, DE Champagne, FA Walker, WR Monfort. (2000). Kinetics and Equilibria in Ligand Binding by Nitrophorins 1?4:? Evidence for Stabilization of a Nitric Oxide?Ferriheme Complex through a Ligand-Induced Conformational Trap.
      Biochemistry 39, 10118-10131
Achieved using the OLIS RSM 1000 SF

J Yu, W Wei, E Danner, JN Israelachvili, JH Waite. (2011). Effects of Interfacial Redox in Mussel Adhesive Protein Films on Mica.
      Advanced Materials 23, 2362-2366
Achieved using the OLIS DSM 1000

RH Pires, SS Venceslau, F Morais, M Teixeira, AV Xavier, IAC Pereira. (2006). Characterization of the Desulfovibrio desulfuricans ATCC 27774 DsrMKJOP Complex-A Membrane Bound Redox Complex Involved in the Sulfate Respiratory Pathway.
      Biochemistry 45, 249-262
Achieved using the OLIS DW-2

S Volden, SM Lystvet, O Halskau, WR Glomm. (2012). Generally applicable procedure for?in situ?formation of fluorescent protein-gold nanoconstructs.
      RSC Advances 2, 11704-11711
Achieved using the OLIS DSM 1000

JT Park, LMG Ramos, AS Bommarius. (2015). Engineering towards Nitroreductase Functionality in Ene-Reductase Scaffolds.
      ChemBioChem DOI:?10.1002/cbic.201402667
Achieved using the OLIS Protein Machine

K Biyani, MA Kahsai, AT Clark, TL Armstrong, SP Edmondson, JW Shriver. (2005). Solution Structure, Stability, and Nucleic Acid Binding of the Hyperthermophile Protein Sso10b2.
      Biochemistry 44, 14217-14230
Achieved using the OLIS DSM 1000

KM Oshaben, R Salari, DR McCaslin, LT Chong, SW Horne. (2012). The Native GCN4 Leucine-Zipper Domain Does Not Uniquely Specify a Dimeric Oligomerization State.
      Biochemistry 51, 9581-9591
Achieved using the OLIS DSM 17

M Lai, KC Yam, D Andrews, and BC Hill. (2011). Copper binding traps the folded state of the SCO protein from Bacillus subtilis.
      Biochim. Biophys. Acta Proteins and Proteomics 1824, 292-302
Achieved using the OLIS DSM 1000

RGC dela Cruz, MA Jairajpuri, SC Bock. (2006). Disruption of a Tight Cluster Surrounding Tyrosine 131 in the Native Conformation of Antithrombin III Activates It for Factor Xa Inhibition .
      J Biol Chem 281, 31668-31676
Achieved using the OLIS DM 45K

SA Cochrane, B Findlay, JC Vederas, ES Ratemi. (2014). Key Residues in Octyl-Tridecaptin A1?Analogues Linked to Stable Secondary Structures in the Membrane.
      ChemBioChem 15, 1295-1299
Achieved using the OLIS DSM 17

CG Brouillette, WJ Dong, ZW Yang, MJ Ray, II Protasevich, HC Cheung, JA Engler. (2005). Forster Resonance Energy Transfer Measurements Are Consistent with a Helical Bundle Model for Lipid-Bound Apolipoprotein A-1.
      Biochemistry 44, 16413-16425
Achieved using the OLIS DSM 1000

CM Timmer, NL Michmerhuizen, AB Witte, MA Van Winkle, D Zhou, K Sinniah. (2014). An Isothermal Titration and Differential Scanning Calorimetry Study of the G-Quadruplex DNA-Insulin Interaction.
      Journal of Physical Chemistry B 118, 1784-1790
Achieved using the OLIS DSM 20

A-C Chien, S Kian, G Zareh, YM Wang, PA Levin. (2012). Changes in the oligomerization potential of the division inhibitor UgtP coordinate?Bacillus subtilis?cell size with nutrient availability.
      Molecular Microbiology 86, 594-610
Achieved using the OLIS DM 45

W Wei, CC Broomwell, JN Israelachvili, JH Waite. (2013). Hydrophobic enhancement of Dopamediated adhesion in a mussel foot protein.
      J. Amer. Chem. Soc. 135, 377-383
Achieved using the OLIS DSM 1000

JK Ma, CJ Carrell, FS Mathews, VL Davidson. (2006). Site Directed Mutagenesis of Proline 52 to Glycine in Amicyanin Converts a True Electron Transfer Reaction into One That is Conformationally Gated.
      Biochemistry 45, 8284-8293
Achieved using the OLIS RSM 1000

J Iwasaki, W-A Smith, SR Stone, WR Thomas, BJ Hales. (2013). Species-Specific and Cross-Reactive IgG1 Antibody Binding to Viral Capsid Protein 1 (VP1) Antigens of Human Rhinovirus Species A, B and C.
      PLoS ONE 8(8): e70552
Achieved using the OLIS DSM 1000

VK Singh, MN Rahman, K Munro, VN Uversky, SP Smith, Z Jia. (2012). Free Cysteine Modulates the Conformation of Human C/EBP Homologous Protein.
      PLoS ONE 7(4): e34680
Achieved using the OLIS DSM 1000

D Li, M Zhu, C Xu, B Ji. (2011). Characterization of the Baicalein-Bovine Serum Albumin Complex without or with Cu2+or Fe3+?by Spectroscopic Approaches.
      Eur. J. Medicinal Chem 46, 588-599
Achieved using the OLIS DSM 1000

IAC Pereira, J LeGall, AV Xavier, M Teixeira. (2000). Characterization of a heme?c?nitrite reductase from a non-ammonifying microorganism,?Desulfovibrio vulgaris?Hildenborough.
      BBA Protein Structure and Molecular Enzymology 1481, 119-130
Achieved using the OLIS DW2

BP Jena. (2011). Role of SNAREs in Membrane Fusion.
      Adv. Exp. Med. Biol. 713, 13-32
Achieved using the OLIS DSM 16

QY He, AB Mason, R Pakdaman, ND Chasteen, BK Dixon, BM Tam, V Nguyen, RTA MacGillivray, RC Woodworth. (2000). Mutations at the Histidine 249 Ligand Profoundly Alter the Spectral and Iron-Binding Properties of Human Serum Transferrin N-Lobe.
      Biochemistry 39, 1205-1210
Achieved using the OLIS RSM 1000 SF

MJ Holden, MP Mayhew, DT Gallagher, VL Vilker. (2002). Chorismate lyase: kinetics and engineering for stability.
      BBA Protein Structure and Molecular Enzymology 1594, 160-167
Achieved using the OLIS RSM 1000

C Fleischer and CK Payne. (2014). Secondary Structure of Corona Proteins Determines the Cell Surface Receptors Used by Nanoparticles.
      Journal of Physical Chemistry B DOI:?10.1021/jp502624n
Achieved using the OLIS DSM 1000

E De Santis, T Hjelmgaard, S Faure, O Roy, C Didierjean, BD Alexander, G Siligardi, R Hussain, T Javorfi, AA Edwards, C Tuillefumier. (2011). Cyclic ?,?-peptoid octamers with differing side chain patterns: synthesis and conformational investigation.
      Amino Acids 41, 663-672
Achieved using the OLIS Custom CD

S Ye, BM Discher, J Strzalka, T Xu, SP Wu, D Noy, I Kuzmenko, T Gog, MJ Therien. (2005). Amphiphilic Four-Helix Bundle Peptides Designed for Light-Induced Electron Transfer Across a Soft Interface.
      Nano Lett 5, 1658-1667
Achieved using the OLIS Cary 14

J Mendez, MM Cruz, YD Reyes, CM Figueroa, EA Orellano, M Morales, A Monteagudo, KH Griebenow. (2014). Delivery of Chemically Glycosylated Cytochrome c Immobilized in Mesoporous Silica Nanoparticles Induces Apoptosis in HeLa Cancer Cells.
      Molecular Pharmaceuticals 11, 102-111
Achieved using the OLIS DSM 10

JO Speare, TS Rush, ME Bloom, B Caughey. (2003). The Role of Helix 1 Aspartates and Salt Bridges in the Stability and Conversion of Prion Protein .
      J Biol Chem 278, 12522-12529
Achieved using the OLIS DSM 16

J Liu, M Meng, C li, X Huang, D Di. (2008). Simultaneous determination of three diarylheptanoids and an ?-tetralone derivative in the green walnut husks (Juglans regia?L.) by high-performance liquid chromatography with photodiode array detector.
      J Chromatography A 1190, 80-85
Achieved using the OLIS DSM 1000

S Laera, G Ceccone, F Rossi, D Gilliland, R Hussain, G Siligardi, L Cazolai. (2011). Measuring Protein Structure and Stability of Protein Nanoparticle Systems with Synchrotron Radiation Circular Dichroism.
      Nano. Lett. 11, 4480-4484
Achieved using the OLIS Custom CD

H Meuzelaar, KA Marino, A Huerta-Viga, MR Panman, LEJ Smeenk, AJ Kettelarij, JH van Maarseveen, P Timmerman, PG Bolhuis, S Woutersen. (2013). Folding Dynamics of the Trp-cage Miniprotein: Evidence for a Native-like Intermediate from combined Time-Resolved Vibrational Spectroscopy and Molecular Dynamics Simulations.
      Journal of Physical Chemistry B DOI: 10.1021/jp404714c
Achieved using the OLIS DSM 1000

M Bertsch, AL Mayburd, RJ Kassner. (2003). The identification of hydrophobic sites on the surface of proteins using absorption difference spectroscopy of bromophenol blue.
      Anal Biochem. 313, 187-195
Achieved using the OLIS Cary 14

T Bartels, LS Ahlstrom, Leftin, F Kamp, C Haass, MF Brown, K Beyer. (2010). The N-Terminus of the Intrinsically Disordered Protein ?-Synuclein Triggers Membrane Binding and Helix Folding?.
      Biophys J 99, 2116-2124
Achieved using the OLIS DSM 20

C-H Chen, G Battaglioli, DL Martin, SA Hobart, W Colon. (2003). Distinctive interactions in the holoenzyme formation for two isoforms of glutamate decarboxylase.
      BBA Proteins and Proteomics 1645, 63-71
Achieved using the OLIS FL SF

J Kardos, B Kiss, A Micsonai, P Rovo, DK Menyhard, J Kovacs, G Varaadi, GK Toth, A Perczel. (2015). Phosphorylation as Conformational Switch from the Native to Amyloid State - Trp-Cage as Protein Aggregation Model.
      Journal of Physical Chemistry B 119, 2946-2955
Achieved using the OLIS GlobalWorks

F Kona, X Xu, P Martin, P Kuzmic, DL Gatti. (2007). Structural and Mechanistic Changes along an Engineered Path from Metallo to Nonmetallo 3-Deoxy-d-manno-octulosonate 8-Phosphate Synthases,.
      Biochemistry 46, 4532-4544
Achieved using the OLIS Cary 60 CD

DJ Adamsm K Holtzman, C Schneider, and MF Butler. (2007). Self-Assembly of Surfactant-like Peptides.
      Langmuir 23, 12729-12736
Achieved using the OLIS DSM 10

JN Tripathy, M Hirasawa, RB Sutton, A Dasgupta, N Vaidyanathan, M Zabet-Moghaddam, FJ Florencio, AP Srivastava, DB Knaff. (2014). A loop unique to ferredoxin-dependent glutamate synthases is not absolutely essential for ferredoxin-dependent catalytic activity.
      Photosynthesis Research 123, 129-139
Achieved using the OLIS DSM 10

AM Preininger, AJ Kimple, DP Siderovski, HE Hamm, TM Iverson. (2009). Helix Dipole Movement and Conformational Variability Contribute to Allosteric GDP Release in G?i Subunits,.
      Biochemistry 48 2630-2642
Achieved using the OLIS DW2000

B Nocek, A Starus, M Makowska-Grzyska, B Gutierrez, S Sanchez, R Jedrejczak, JC Mack, KW Olsen, A Joachimiak, RC Holz. (2014). The Dimerization Domain in DapE Enzymes Is required for Catalysis.
      PlosOne 9, e93593
Achieved using the OLIS DSM 20

Y Yue, X Chen, J Qin, X Yao. (2009). Characterization of the mangiferin?human serum albumin complex by spectroscopic and molecular modeling approaches.
      J Pharm. Biomed. Analysis 49, 753-759
Achieved using the OLIS DSM 1000

D Apiyo, J Guidry, and P Wittung-Stafshede. (2000). No cofactor effect on equilibrium unfolding of?Desulfovibrio desulfuricansflavodoxin.
      BBA Protein Structure and Molecular Biology 1479, 214-224
Achieved using the OLIS DSM 1000

SB Shields SJ Franklin. (2004). De Novo Design of a Copper (II)-Binding Helix-Turn-Helix Chimera: The Prion Octarepeat Motif in a New Context.
      Biochemistry 43, 16086-16091
Achieved using the OLIS DSM 17

RD Gray, L Petraccone, JO Trent, JB Chaires. (2009). Characterization of a K+-Induced Conformational Switch in a Human Telomeric DNA Oligonucleotide Using 2-Aminopurine Fluorescence.
      Biochemistry 49, 179-194
Achieved using the OLIS RSM 1000

V Garcia-Gonzalez and J Mas-Oliva. (2013). Amyloid fibril formation of peptides derived from the C-terminus of CETP modulated by lipids.
      Biochem. Biophys. Res. Comm. 434, 54-59
Achieved using the OLIS DM 45

F Desmet, A Berczi, L Zimanyi, H Asard. (2011). Axial ligation of the high-potential heme center in an?Arabidopsis?cytochrome?b561.
      FEBS Letters 585, 545-548
Achieved using the OLIS DW 2000

ML Lolli, C Giordano, DS Pickering, B Rolandor, KB Hansen, A Foti, A Contreras-Sanz, A Amir, R Fruttero, A Gasco, B Nielsen, TN Johansen. (2010). 4-Hydroxy-1,2,5-oxadiazol-3-yl Moiety as Bioisoster of the Carboxy Function. Synthesis, Ionization Constants, and Molecular Pharmacological Characterization at Ionotropic Glutamate Receptors of Compounds Related to Glutamate and Its Homologues.
      J. Med. Chem. 53, 4110-4118
Achieved using the OLIS DSM 10

J Song, W Zhang, M Kai, J Chen, R Liang, X Zheng, G Li, B Zhang, K Wang, Y Zhang, Z Yang, J ni, R Wang. (2013). Design of Acid-activated Antimicrobial Peptide for Tumor Therapy.
      Molecular Pharmaceutics 10, 2934-2941
Achieved using the OLIS DSM 1000

T Sato, P Pallavi, U Golebiewska, S McLaughlin, SO Smith. (2006). Structure of the Membrane Reconstitution Transmembranes Juxtamembrane Peptide EGFR(622-660) and its Interactions with Ca2+/Calmodulin.
      Biochemistry 45, 12704-12714
Achieved using the OLIS DSM 1000

B McMicken, RJ Thomas, L Brancaleon. (2013). Photoinduced partial unfolding of tubulin bound to meso-tetrakis(sulfonatophenyl) porphyrin leads to inhibition of microtubule formation in vitro.
      Journal of Biophotonics 7, 874-888
Achieved using the OLIS DSM 17

M Xu, Z -R Ma, L Huang, F -J Chen, Z -Z Zeng. (2011). Spectroscopic studies on the interaction between Pr(III) complex of an ofloxacin derivative and bovine serum albumin or DNA.
      Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 78, 503
Achieved using the OLIS DSM 1000

G Hernandez-Cancel, D Suazo-Davila, J Medina-Guzman, M Rosado-Gonzalez, LM Diaz-Vazquez, K Griebenow. (2014). Chemically glycosylation improves the stability of an amperometric horseradish peroxidase biosensor.
      Analytica Chimica Acta 854, 129-139
Achieved using the OLIS DSM 10

NA Tavenor, KI Silva, S Saxena, WS Horne. (2014). Origins of Structural Flexibility in Protein-Based Supramolecular Polymers Revealed by DEER Spectroscopy.
      Journal of Physical Chemistry B 118, 9881-9889
Achieved using the OLIS DSM 17

CT Lohans. KM Towle, M Miskolzie, RT McKay, MJ van Belkum, LM McMullen, JC Vederas. (2013). Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide.
      Biochemistry 52, 3987-3994
Achieved using the OLIS DSM 17

DJ Hill and JS Moore. (2002). Helicogenicity of solvents in the conformational equilibrium of oligo(m-phenylene ethynylene)s: Implications for foldamer research .
      PNAS 99, 5053-5057
Achieved using the OLIS Cary 17

X Jing, M Yang, MR Kasimova, M Malmsten, H Franzyk, L Jorgensen, C Foged, HM Nielsen. (2012). Membrane adsorption and binding, cellular uptake and cytotoxicity of cell-penetrating peptidomimetics with ?-peptide/?-peptoid backbone: Effects of hydrogen bonding and ?-chirality in the ?-peptoid residues.
      Biochim. Biophys. Acta Biomembranes 1818, 2660-2668
Achieved using the OLIS DSM 10

TC Bjorndahl, G-P Zhou, X Liu, R Perez-Pineiro, V Semenchenko, F Saleem, S Acharya, A Bujold, CA Sobsey, DS Wishart. (2011). Detailed Biophysical Characterization of the Acid-induced PrPc to PrP Conversion Process.
      Biochemistry 50, 1162-1173
Achieved using the OLIS DSM 17

E Szymanska, K Frydenvang, A Contreras-Sanz, DS Pickering, E Frola, Z Serafimoska, B Nielsen, JS Kastrup, TN Johansen. (2011). A new phenylalanine derivative acts as an antagonist at the AMPA receptor GluA2 and introduces partial domain closure: Synthesis, resolution, pharmacology and crystal structure.
      J. Med. Chem. 54, 7289-7298
Achieved using the OLIS DSM 10

RB Prince, SA Barnes, JS Moore. (2000). Foldamer-Based Molecular Recognition.
      JACS 122, 2758-2762
Achieved using the OLIS DSM 17

DS Silva, LMG Pereira, AR Moreira, F Ferreira-da-Silva, RM Brito, TQ Faria, I Zornetta, C Montecucco, P Oliveira, JE Azevedo, PJB Pereira, S Macedo-Ribeira, A Do Vale, NMS do Santos. (2013). The Apoptogenic Toxin AIP56 Is a Metalloprotease A-B Toxin that Cleaves NF-?b P65.
      PLoS Pathog 9(2): e1003128
Achieved using the OLIS DSM 20

JT Welch, WR Kearney, SJ Franklin. (2003). Lanthanide-binding helix-turn-helix peptides: Solution structure of a designed metallonuclease .
      PNAS 100, 3725-3730
Achieved using the OLIS DSM 17

GW Bos, A Trullas-Jimeno, W Jiskoot, DJA Crommelin, WE Hennink. (2000). Sterilization of Poly(dimethylamino)ethyl Methacrylate-based Gene Transfer Complexes.
      Int. J. Pharmaceutics 211, 79-88
Achieved using the OLIS DSM 1000

L -S Huang, JT Shen, AC Wang, EA Berry. (2006). Crystallographic studies of the binding of ligands to the dicarboxylate site of Complex II, and the identity of the ligand in the ?oxaloacetate-inhibited? state.
      BBA Bioenergetics 1757, 1073-1083
Achieved using the OLIS DW 2

V Samoylenko, M Rahman, BL Tekwani, LM Tripathi, YH Wang, SI Khan, IA Khan, LS Miller, VC Joshi, I Muhammad. (2010). Banisteriopsis caapi, a unique combination of MAO inhibitory and antioxidative constituents for the activities relevant to neurodegenerative disorders and Parkinson's disease.
      J Ethnopharmacology 127, 357-367
Achieved using the OLIS DSM 20

A Tiwari and P Pospisil. (2009). Superoxide oxidase and reductase activity of cytochrome b559 in photosystem II.
      BBA-Bioenergetics 1787, 985-994
Achieved using the OLIS RSM 1000F

DMAM Luykx, SS Goerdayal, PJ Dingemanse, W Jiskoot, PMJM Jongen. (2005). HPLC and Tandem Detection to Monitor Conformational Properties of Biopharmaceuticals.
      J Chromatography B 821, 45-52
Achieved using the OLIS DSM 1000

LA Martin-Visscher, T Sprules, LJ Gursky, JC Vederas. (2008). Nuclear Magnetic Resonance Solution Structure of PisI, a Group B Immunity Protein that Provides Protection Against the Type IIa Bacteriocin Piscicolin 126, PisA.
      Biochemistry 47, 6427-6436
Achieved using the OLIS DSM 17

JJ Guidry and P Wittung-Stafshede. (2002). Low stability for monomeric human chaperonin protein 10: interprotein interactions contribute majority of oligomer stability.
      ABB 405, 280-282
Achieved using the OLIS DSM 16

CB Marshall, A Chakrabartty, PL Davies. (2005). Hyperactive Antifreeze Protein from Winter Flounder Is a Very Long Rod-like Dimer of ?-Helices .
      J Biol Chem 280, 17920-17929
Achieved using the OLIS DSM 1000

R Roychaudhuri, G Sarath, M Zeece, J Markwell. (2004). Stability of the allergenic soybean Kunitz trypsin inhibitor.
      BBA Proteins and Proteomics 1699, 207-212
Achieved using the OLIS DSM 1000

L Qin, DA Mills, C Hiser, A Murphree, RM Gravito, S Ferguson-Miller, J Hosler. (2007). Crystallographic Location and Mutational Analysis of Zn and Cd Inhibitory Sites and Role of Lipidic Carboxylates in Rescuing Proton Path Mutants in Cytochrome C Oxidase.
      Biochemistry 46, 6239-6248
Achieved using the OLIS RSM 1000

J Song, M Kai, W Zhang, J Zhang, L Liu, B Zhang, X Liu, R Wang. (2011). Cellular uptake of transportan 10 and its analogs in live cells: Selectivity and structure?activity relationship studies.
      Peptides 32, 1934-1941?
Achieved using the OLIS DSM 1000

DT Derksen, JL Stymiest, JC Vederas. (2006). Antimicrobial Leucocin Analogues with a Disulfide Bridge Replaced by a Carbocycle or by Noncovalent Interactions of Allyl Glycine Residues.
      JACS 128, 14252-14253
Achieved using the OLIS DSM 17

AJ van Hell, A Klymchencko, PP Burgers, EE Moret, W Jiskoot, WE Hennink, DJA Crommelin, E Mastrobattista. (2010). Conformation and Intermolecular Interactions of SA2 Peptides Self-Assembled into Vesicles.
      J Phys Chem 114, 11046-11052
Achieved using the OLIS DSM 1000

Z Ye, D Bayron, JJ Aguilera, S Srinivasan, Y Wang, LC Serpell, W Colon. (2011). Inflammation protein SAA2.2 spontaneously forms marginally stable amyloid fibrils at physiological temperature.
      Biochemistry 50, 9184-9191
Achieved using the OLIS DSM 20

EG Bellomo, P Davidson, M Imperor-Clerc, TJ Deming. (2004). Aqueous Cholesteric Liquid Crystals Using Uncharged Rodlike Polypeptides.
      JACS 126, 9101-9105
Achieved using the OLIS DSM 1000

I Pozdnyakova, J Guidry, P Wittung-Stafshede. (2002). Studies of?Pseudomonas aeruginosa?Azurin Mutants: Cavities in??-Barrel Do Not Affect Refolding Speed.
      Biophys J 82, 2645
Achieved using the OLIS DSM 1000

S Chen, FA Ferrone, R Wetzel. (2002). Huntington's disease age-of-onset linked to polyglutamine aggregation nucleation .
      PNAS 99, 11884-11889
Achieved using the OLIS DSM 1000

CA Harley, CSH Jesus, R Carvalho, RMM Brito, JH Morais-Cabral. (2012). Changes in Channel Trafficking and Protein Stability Caused by LQT2 Mutations in the PAS Domain of the HERG Channel.
      PLoS ONE 7(3): e32654
Achieved using the OLIS DSM 20

N Nakatsuka, SN Barnaby, A Tsiola, KR Fath, BA Williams, IA Banerjee. (2012). Self-Assembling Peptide Assemblies bound to ZnS Nanoparticles and their Interactions with Mammalian Cells.
      Colloids and Surfaces B: Biointerfaces 103, 405-415
Achieved using the OLIS DSM 20

S Chen, V Berthelier, JB Hamilton, B O'Nuallain, T Wetzel. (2002). Amyloid-like Features of Polyglutamine Aggregates and Their Assembly Kinetics.
      Biochemistry 41, 7391-7399
Achieved using the OLIS DSM 1000

W Soliman, L Wang, S Bhattacharjee, K Kaur. (2011). Structure?Activity Relationships of an Antimicrobial Peptide Plantaricin S from Two-Peptide Class IIb Bacteriocins.
      J. Med. Chem 54, 2399-2408
Achieved using the OLIS DSM 17

SG Kim, YH Chi, J-S Lee, SR Schlesinger, DB Knaff, ST Kim. (2010). Redox properties of a thioredoxin-like Arabidopsis protein, AtTDX?.
      BBA Proteins and Proteomics 1804, 2213-2221
Achieved using the OLIS DSM 10

JJ Aguilera, F Zhang, JM Beaudet, RJ Linhardt, W Colon. (2014). Divergent effect of glycosaminoglycans on the?in?vitroaggregation of serum amyloid A.
      Biochimie 104, 70-80
Achieved using the OLIS DSM 1000

J Shearer and P Soh. (2007). The Copper(II) Adduct of the Unstructured Region of the Amyloidogenic Fragment Derived from the Human Prion Protein is Redox-Active at Physiological pH.
      Inorg. Chem. 46, 710-719
Achieved using the OLIS DSM 17

VK Singh and Z Jia. (2005). Refolding and one-step purification of recombinant human ARA70 over-expressed in?Escherichia coli.
      Protein Expression and Purification 39, 283-287
Achieved using the OLIS DSM 1000

R Soudy, A Gill, T Sprules, A Lavasanifar, K Kaur. (2011). Proteolytically Stable Cancer Targeting Peptides with High Affinity for Breast Cancer Cells.
      J. Med. Chem. 54, 7523-7534
Achieved using the OLIS DSM 17

Y Li, H Wang, B Jia, C Liu, Y Xiao, Q Li. (2012). A?study?of the?interaction?of?deoxynivalenol?with?human?serum?albumin?by?spectroscopic?technique?and?molecular?modeling.
      manuscript.govi.de/manuscripts/5435/18377.doc
Achieved using the OLIS DSM 1000

KE Pavia, SA Spinella, DE Elmore. (2012). Novel histone-derived antimicrobial peptides use different antimicrobial mechanisms.
      Biophys. Biochim. Acta 1818, 869-876
Achieved using the OLIS Mini Protein Machine

L-L Liu, J-L Yang, Y-P Shi. (2011). Sesquiterpenoids and other constituents from the flower buds of Tussilago farfara.
      J. Asian Nat. Prod. Res. 13, 920-929
Achieved using the OLIS DSM 1000

T Javorfi, R Hussain, D Myatt, G Siligardi. (2010). Measuring circular dichroism in a capillary cell using the b23 synchrotron radiation CD beamline at diamond light source.
      Chirality 22, E149-E153
Achieved using the OLIS Custom CD

MB McNeil and PC Fineran. (2013). The conserved RGxxE motif of the bacterial FAD assembly factor SdhE is required for succinate dehydrogenase flavinylation and activity.
      Biochemistry 52, 7628-7640
Achieved using the OLIS DSM 10

JJ Adams, BA Webb, HL Spencer, SP Smith. (2005). Structural Characteization of Type II Dockerin Module from the Cellulosome of Clostridium thermocellum: Calcium-Induced Effects on Conformational Recognition.
      Biochemistry 44, 2173-2182
Achieved using the OLIS DSM 1000

F Eker, K Griebenow, X Cao, LA Naifie, R Schweitzer-Stenner. (2003). Stable Conformations of Tripeptides in Aqueous Solution Studied by UV Circular Dichroism Spectroscopy.
      JACS 125, 8178-8185
Achieved using the OLIS DSM 10

NG James, CL Berger, SL Byrne, VC Smith, TA MacGiiivray, AB Mason. (2007). Intrinsic Fluorescence Reports a Global Conformational Change in the N-Lobe of Human Serum Transferrin following Iron Release?.
      Biochemistry 46 10603-10611
Achieved using the OLIS RSM 1000 SF

JN Lampe, SN Floor, JD Gross, CR Nishida, Y Jiang, MJ Trnka, PR Ortiz de Montellano. (2008). Ligand-Induced Conformational Heterogeneity of Cytochrome P450 CYP119 Identified by 2D NMR Spectroscopy with the Unnatural Amino Acid 13C-p-Methoxyphenylalanine.
      JACS 130, 16168-16169
Achieved using the OLIS DW2

NR Maddux, IT Rosen, L Hu, CM Olsen, DB Volkin, CR Middaugh. (2012). An improved methodology for multidimensional high-throughput preformulation characterization of protein conformational stability.
      J. Phar,. Sci. 101, 2017-2024
Achieved using the OLIS Protein Machine

EM Maes, SA Roberts, A Weichsel, WR Montfort. (2005). Ultrahigh Resolution Structures of Nitrophorin 4: Heme Distortion in Ferrous CO NO Complexes.
      Biochemistry 44, 12690-12699
Achieved using the OLIS Kin-fit

S Srinivasan, S Patke, Y Wang, Z Ye, J Litt, SK Srivastava, MM Lopez, D Kurouski, IK Lednev, RS Kane, W Colon. (2013). Pathogenic Serum Amyloid A 1.1 Shows a Long Oligomer-rich Fibrillation Lag Phase Contrary to the Highly Amyloidogenic Non-pathogenic SAA2.2.
      J. Biol. Chem. 288, 2744-2755
Achieved using the OLIS DSM 1000

Y Qu and DW Bolen. (2002). Efficacy of macromolecular crowding in forcing proteins to fold.
      Biophysical Chemistry 101, 155-165
Achieved using the OLIS DSM 1000

MA Hummel, PM Gannett, J Aguilar, TS Tracy. (2008). Intrinsically Disordered Human C/EBP Homologous Protein Regulates Biological Activity of Colon Cancer Cells during Calcium Stress.
      J Mol Biol 380, 313-326
Achieved using the OLIS DSM 1000

PJ Reeder, Y-M, Huang, JS Dordick, C Bystroff. (2010). A Re-wired Green Fluorescent Protein: Folding and Function in a Non-sequential, Non-circular GFP Permutant.
      Biochemistry 49, 10773-10779
Achieved using the OLIS DSM 10

JA Kyndt, JC Fitch, TE Meyer, MA Cusanovich. (2005). Thermochromatium tepidum Photoactive Yellow Protein/Bacteriophytochrome/Diguanylate Cyclase: Characterization of the PYP Domain.
      Biochemistry 44, 4755-4764
Achieved using the OLIS Kin-Fit

KE Schaefer, P Keller, TJ Deming. (2006). Thermotropic Polypeptides Bearing Side-On Mesogens.
      Macromolecules 39, 12-22
Achieved using the OLIS DSM 1000

R Soudy, L Wang, K Kaur. (2012). Synthetic Peptides Derived from the Sequence of a Lasso Peptide Microcin J 25 Show Antibacterial Activity.
      Bioorg. Med. Chem 20, 1794-1800
Achieved using the OLIS DSM 17

A Ramzi, M Sutter, WE Hennink, W Jiskoot. (2006). Static Light Scattering and Small Angle Beutron Scattering DSM 1000 Study on Aggregated Recombinant Gelatin in Aqueous Solution.
      J Pharmaceutical Sciences 95, 1703-1711
Achieved using the OLIS DSM 1000

P Fonte, JC Andrade, V Seabra, B Sarmento. (2012). Chitosan-Based Nanoparticles as Delivery Systems of Therapeutic Proteins.
      Methods in Molecular Biology 899, 471-487
Achieved using the OLIS DSM 20

R Roychaudhuri, G Sarath, M Zeece, J Markwell. (2003). Reversible denaturation of the soybean Kunitz trypsin inhibitor.
      ABB 412, 20-26
Achieved using the OLIS CD

CH Tsai, TY Fang, NT Ho, C Ho. (2000). Novel Recombinant Hemoglobin, rHb (?N108Q), with Low Oxygen Affinity, High Cooperativity, and Stability against Autoxidation.
      Biochemistry 39, 13719-13729
Achieved using the OLIS Olis SF

F Khan, W Li, S Habelitz. (2012). Biophysical characterization of synthetic amelogenin C-terminal peptides.
      Eu. J. Oral Sci. 120, 113-122
Achieved using the OLIS DSM 20

E Ritter, M Elgeti, KP Hofman, FJ Bartl. (2007). Deactivation and Proton Transfer in Light-induced Metarhodopsin II/Metarhodopsin III Conversion: A TIME-RESOLVED FOURIER TRANSFORM INFRARED SPECTROSCOPIC STUDY .
      J Biol Chem 282, 10720-10730
Achieved using the OLIS RSM 1000

MV Frassa, M Ceolin, MS Dreon, H Heras. (2010). Structure and stability of the neurotoxin PV2 from the eggs of the apple snailPomacea canaliculata.
      BBA Proteins and Proteomics 1804, 1492-1499
Achieved using the OLIS SLM 4800?

H-S Seo, Y Ma, EW Debler, D Wacker, S Kutik, G Blobel, A Hoelz. (2009). Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex.
      PNAS 106, 14281-14286
Achieved using the OLIS RSM 1000

W Liu, Y Kamensky, R Kakkar, E foley, RJ Kulmacz, G Palmer. (2005). Purification and characterization of bovine adrenal cytochrome?b561?expressed in insect and yeast cell systems.
      Protein Expression and Purification 40, 429-439
Achieved using the OLIS ?????

ML Reback. (2010). Development of a Stopped-Flow Ultra-Rapid-Scanning Fourier Transform Infrared Spectrometer and its Application for Kinetic and Mechanistic Studies of Inorganic Compounds.
      Ph.D. Thesis Univ Idaho (3440782)
Achieved using the OLIS GlobalWorks

R Bizzarri, M Serresi, F Cardarelli, S Abbruzzetti, B Campanini, C Viappiani, F Beltran. (2009). Single Amino Acid Replacement Makes Aequorea victoria Fluorescent Proteins Reversibly Photoswitchable.
      JACS 132, 85-95
Achieved using the OLIS RSM 1000 with AP SF

KL Stewart, ED Dodds, VH Wysocki, MHJ Cordes. (2013). A polymetamorphic protein.
      Protein Science 22, 641-649
Achieved using the OLIS DSM 20

D Mitra, X Yang, K Moffat. (2012). Crystal Structures of Aureochrome1 LOV Suggest New Design Strategies for Optogenetics.
      Structure 20, 698-706
Achieved using the OLIS GlobalWorks

BC Hill and D Andrews. (2011). Differential affinity of BsSCO for Cu(II) and Cu(I) suggests a redox role in copper transfer to the CuA center of cytochrome c oxidase?.
      Biochim. Biophys. 1817, 948-954
Achieved using the OLIS DSM 1000

J Zhou, C Wei, G Jia, X Wang, Z Feng, C Li. (2009). Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics.
      Biochemie 91, 1104-1111
Achieved using the OLIS DSM 1000

ED Micewicz, AL cole, C-L Jung, H Luong, ML Phillips, P Pratikhya, s Sharma, AJ Waring, AM Cole, P Ruchala. (2010). Grifonin-1: A Small HIV-1 Entry Inhibitor Derived from the Algal Lectin, Griffithsin.
      PLoS ONE 5(12):e14360
Achieved using the OLIS GlobalWorks

M Xu, F -J Chen, L Huang, P-X Xi, Z -Z Zhang. (2011). The binding of rare earth metal complexes with an ofloxacin derivative to bovine serum albumin and its effect on the conformation of protein.
      J Luminescence 131, 1557-1565
Achieved using the OLIS DSM 1000

EP Holowka, DJ Pochan, TJ Deming. (2005). Charged Polypeptide Vesicles with Controllable Diameter.
      JACS 127, 12423-12428
Achieved using the OLIS DSM 1000

JM Reis, D Burns, GA Woolley. (2014). Optical control of protein-protein interactions via blue light induced domain swapping.
      Biochemistry 53, 5008-5016
Achieved using the OLIS DSM 1000

J-X Yan, K-R Wang, R Chen, J-J Song, B-Z Zhang, W Dang, W Zhang, R Wang. (2012). Membrane active antitumor activity of NK-18, a mammalian NK-lysin-derived cationic antimicrobial peptide.
      Biochimie 94, 184-191
Achieved using the OLIS DSM 1000

E Salsi, R Guan, B Campanini, S Bettati, J Lin, PF Cook, A Mozzarelli. (2011). Exploring?O-acetylserine sulfhydrylase-B isoenzyme from?Salmonella typhimurium?by fluorescence spectroscopy.
      Arch. Biochem. Biophys 505, 178-185
Achieved using the OLIS RSM 1000

S Runa, A Hill, VL Cochran, CK Payne. (2014). PEGylated nanoparticles: protein corona and secondary structure.
      Proc. SPIE9165, Physical Chemistry of Interfaces and Nanomaterials XIII, 91651F?
Achieved using the OLIS DSM 17

LYJGM de Oca, A Chagollo-Lopez, LG de la Vara, T Cabellos-Avelar, C Gomez-Lojero, EB Gitierez-Cirlos. (2012). The composition of the Bacillus subtilis aerobic respiratory chain supercomplexes.
      J. Bioenergetics and Biomembranes 44, 473-486
Achieved using the OLIS DW2

ED Prieto, N Ramella, LA Cuallar, MA Tricerri, HA Garda. (2012). Characterization of a Human Apolipoprotein A-I Construct Expressed in a Bacterial System.
      The Protein Journal 31, 681-688
Achieved using the OLIS SLM 4800

Y Wang, S Srinivasan, Z Ye, J Aguilera, MM Lopez, W Colon. (2011). Serum amyloid A 2.2 refolds into a octameric oligomer that slowly converts to a more stable hexamer.
      Biochem. Biophys. Res. Comm 407, 725-729
Achieved using the OLIS DSM 1000

HA Davies, J Madine, DA Middleton. (2015). Comparisons with amyloid-? reveal an aspartate residue that stabilizes fibrils of the aortic amyloid peptide medin.
      Journal of Biological Chemistry 290, 7791-7803
Achieved using the OLIS DSM 20

MJ Ranaghan, CT Schwall, NN Alder, RR Birge. (2011). Green Proteorhodopsin Reconstituted into Nanoscale Phospholipid Bilayers (Nanodiscs) as Photoactive Monomers.
      J. Amer. Chem. Soc. 133, 18318-18327
Achieved using the OLIS RSM 1000 (laser)

SM Lystvet, S Volden, G Singh, IM Rundgren, H Wen, O Halskau, WR Glomm. (2013). Anticancer Activity from Gold-alpha-Lactalbumin Nanoconstructs?.
      J. Phys. Chem. C 117, 2230-2238
Achieved using the OLIS DSM 1000

J Guidry and P Wittung-Stafshede. (2004). First characterization of co-chaperonin protein 10 from hyper-thermophilic?Aquifex aeolicus.
      BBRC 317, 176-180
Achieved using the OLIS ????

S Griffin, A Vitello, P Wittung-Strafshede. (2002). Buried water molecules contribute to cytochrome f stability.
      ABB 404, 335-337
Achieved using the OLIS CD

Y Delgado, M Morales-Cruz, J Hernandez-Roman, Y Martinez, K Griebenow. (2014). Chemical glycosylation of cytochrome c improves physical and chemical protein stability.
      Biomed Central 147-2091-15-16
Achieved using the OLIS DSM 10

Y Yue, X Chen, J Qin, X Yao. (2009). Characterization of interaction between C.I. Acid Green 1 and human serum albumin: Spectroscopic and molecular modeling method.
      Dyes and Pigments 83, 148-154
Achieved using the OLIS DSM 1000

A Kumar, DC Burns, MS Al-Abdul-Wahid, GA Woolley. (2013). A circularly permuted photoactive yellow protein as a scaffold for photoswitch design.
      Biochemistry 52, 3320-3331
Achieved using the OLIS DSM 1000

R Schweitzer-Stenner, T Measley, L Kakalis, F Jordan, S Pizzanelli, C Forte, K Griebenow. (2007). Conformations of Alanine-Based Peptides in Water Probed by FTIR, Raman, Vibrational Circular Dichroism, Electronic Circular Dichroism, and NMR Spectroscopy.
      Biochemistry 46, 1587-1596
Achieved using the OLIS DSM 10

J Holm, M Ferreras, H Ipsen, PA Wutzen, M Gajhede, JN Larsen, K Lund, MD Spangford. (2011). Epitope Grafting, Re-creating a Conformational Bet v 1 Antibody Epitope on the Surface of the Homologous Apple Allergen Mal d 1.
      J. Biol. Chem. 286, 17569-17568
Achieved using the OLIS DSM 10

RS Phillips, N Johnson AV Kanath. (2002). Formation in Vitro of Hybrid Dimers of H463F and Y74F Mutant Escherichia coli Tryptophan Indole-lyase Rescues Activity with l-Tryptophan.
      Biochemistry 41, 4012-4019
Achieved using the OLIS RSM 1000 SF

D Missirlis, M Farine, M Kastantin, B Anthanarayanan, T Neumann, M Tirrell. (2010). Linker Chemistry Determines Secondary Structure of p5314?29 in Peptide Amphiphile Micelles.
      Bioconjugate Chem. 21, 465-475
Achieved using the OLIS DSM 1000

Y Tutar and JG Harmon. (2006). Effect of salt bridge on transcription activation of CRP-dependent lactose operon in Escherichia coli.
      ABB 453, 217-223
Achieved using the OLIS DSM 1000

T Yanetani, S Park, A Tsuneshige, K Imai, K Kanaori. (2002). Global Allostery Model of Hemoglobin: MODULATION OF O2 AFFINITY, COOPERATIVITY, AND BOHR EFFECT BY HETEROTROPIC ALLOSTERIC EFFECTORS .
      J Biol Chem 277, 34508-34520
Achieved using the OLIS Cary 118

LA Cueller, ED Prieto, LV Cabaleiro, HA Garda. (2014). Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process.
      Biochimica Biophysica Acta Molecular and Cell Biology of Lipids 1841, 180-189
Achieved using the OLIS SLM 4800

SP Balashov, ES Imasheva, AR Choi, K-H Jung, S Liaaen-Jensen, JK Lanyi. (2010). Reconstitution of Gloeobacter Rhodopsin with Echinenone: Role of the 4-keto Group.
      Biochem. 49, 9792-9799
Achieved using the OLIS SLM 8000

R Xu, A Ori, TR Rudd, KA Uniewicz, YA Ahmed, SE Guimond, MA Skidmore, G Siligardi, EA Yates, DG Fernig. (2012). Diversification of the structural determinants of fibroblast growth factor-heparin interactions; implications for binding specificity.
      J. Biol. Chem. 287, 40061-40073
Achieved using the OLIS DSM 20

VS Sobolev, SA Neff, JB Gloer. (2009). New Dimeric Stilbenoids from Fungal-Challenged Peanut (Arachis hypogaea) Seeds.
      J. Agric. Food Chem 58, 875-881
Achieved using the OLIS DSM 17

J Pilbrow, M Sabherwal, D Garama, A Carne. (2014). A Novel Fatty Acid-Binding Protein-Like Carotenoid-Binding Protein from the Gonad of the New Zealand Sea Urchin?Evechinus chloroticus.
      PLoS ONE 9(9): e106465
Achieved using the OLIS DSM 10

K Nienhaus, JM Kriegl, GU Nienhaus. (2004). Structural Dynamics in the Active Site of Murine Neuroglobin and Its Effects on Ligand Binding .
      J Biol Chem 279, 22944-22952
Achieved using the OLIS Cary 14

F-H Liu, PL Davies, LA Graham. (2011). Thr- and Ala-Rich Hyperactive Antifreeze Protein From Inchworm Folds as a Flat Silk-Like Beta-Helix.
      Biochemistry 510, 4467-4478
Achieved using the OLIS DSM 1000

FG Pearce, SH MackIntosh, JA Gerrard. (2007). Formation of Amyloid-like Fibrils by Ovalbumin and Related Proteins under Conditions Relevant to Food Processing.
      J. Agric. Food. Chem. 55, 318-322
Achieved using the OLIS DSM 10

SW Crawley, J Liburd, K Shaw, Y Jung, SP Smith, GP Cote. (2011). Identification of Calmodulin and MlcC as Light Chains for Dictyostelium Myosin-I Isozymes.
      Biochemistry 50, 6579-6588
Achieved using the OLIS DSM 1000

TD Ju, AS Woods, RJ Cotter, P Moenne-Loccoz, KD Karlin. (2000). Dioxygen and nitric oxide reactivity of a reduced heme/non-heme diiron(II) complex [(5L)FeIIcdots, three dots, centeredFeII---Cl]+. Using a tethered tetraarylporphyrin for the development of an active site reactivity model for bacterial nitric oxide reductase.
      Inorganica Chimica Acta 297, 362-372
Achieved using the OLIS HPDA 8452

JP Hunn, S Koenen-Waisman, N Papic, N Schroeder, NP Pawlowski, R Lange, F Kaiser, J Zerrahn, S Martens, JC Howard. (2008). Regulatory Interactions between IRG Resistance GTPases in the Cellular Response to Toxoplasma gondii.
      EMBO J 1-15
Achieved using the OLIS DM 45

KML Rose, Z Wang, GN Magrath, ES Hazzard, JD Hildebradt, KL Schey. (2008). Aquaporin 0?Calmodulin Interaction and the Effect of Aquaporin 0 Phosphorylation.
      Biochemistry 47, 339-347
Achieved using the OLIS DM 45

L Leon, P Logrippo, R Tu. (2010). elf-Assembly of Rationally Designed Peptides under Two-Dimensional Confinement?.
      Biophys J 99, 2888
Achieved using the OLIS DSM 20

KR Brzezinska, SA Curtin, TJ Deming. (2002). Polypeptide End-Capping Using Functionalize Isocyanates: Preparation of Pentablock Copolymers.
      Macromolecues 35, 2970-2976
Achieved using the OLIS DSM 1000

AP Srivastava, M Hirasawa, M Bhalla, J-S Chung, JP Allen, MK Johnson, JN Tripathy, LM Rubio, BJ Vaccaro, S Subramanian, E Flores, M Zabel-Moghaddam, K Stittle, DB Knaff. (2013). The Roles of Four Conserved Basic Amino Acids in a Ferredoxin-Dependent Cyanobacterial Nitrate Reductase.
      Biochemistry 52, 4343-4353
Achieved using the OLIS DSM 10

NR Maddux, SB Joshi, DB Volkin, JP Ralson, R Middaugh. (2011). Multidimensional methods for the formulation of biopharmaceuticals and vaccines.
      J. Pharm. Sci. 100, 4171-4197
Achieved using the OLIS Protein Machine

SA Roberts, A Weichsel, G Grass, K Thakali, JT Hazzard, G Tollin, C Rensing, WR Monfort. (2002). Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli .
      PNAS 99, 2766-2771
Achieved using the OLIS KinFit

D Li, X Cao, B Ji. (2010). Spectrophotometric studies on the interaction between myricetin and lysozyme in the absence or presence of Cu2+or Fe3+.
      J Luminescence 130, 1893
Achieved using the OLIS DSM 1000

JS Bahnsen, H Franzyk, AS Sandberg-Schaal, HM Nielsen. (2012). Antimicrobial and cell-penetrating properties of penetratin analogs: Effect of sequence and secondary structure.
      Biochim. Biophys. Acta 1828, 223-232
Achieved using the OLIS DSM 10

JW Kyle, VM Berthoud, J Kurutz, PJ Minogue, M Greenspan, DA Hanck, EC Beyer. (2009). The N Terminus of Connexin37 Contains an ?-Helix That Is Required for Channel Function .
      J Biol Chem 284, 20418-20427
Achieved using the OLIS Software

TE Angel, PC Kraft, EA Dratz. (2006). Metarhodopsin-II stabilization by crosslinked Gt??C-terminal peptides and implications for the mechanism of GPCR-G protein coupling.
      Vision Research 46, 4547-4555
Achieved using the OLIS UV 3000

F Eker, K Griebenow, X Cao, LA Naifie, R Schweitzer-Stenner. (2004). Tripeptides with Ionizable Side Chains Adopt a Perturbed Polyproline II Structure in Water.
      Biochemistry 43, 613-621
Achieved using the OLIS DSM 10

VA Muniz, S Srinivasan, SA Boswell, DW Meinhold, T Chils, E Osuna, W Colon. (2010). The role of the local environment of engineered Tyr to Trp substitutions for probing the denaturation mechanism of FIS.
      Prot. Sci. 20, 302-312
Achieved using the OLIS DSM 10

T Lou. (2011). The Synthesis and Structural Studies of Oligoproline Macrocyles.
      Master's Thesis Univ. Toronto
Achieved using the OLIS RSM 1000

KL Stewart, MR Nelson, KV Eaton, WJ Anderson, MHJ Cordes. (2013). A role for indels in the evolution of cro protein folds.
      Proteins: Atructure, Function, and Bioinformatics 81, 1988-1996
Achieved using the OLIS DSM 20

KM Oshaben and WS Horne. (2014). Tuning Assembly Size in Peptide-Based Supramolecular Polymers by Modulation of Subunit Association Affinity.
      Biomacromolecules 15, 1436-1442
Achieved using the OLIS DSM 17

PD Mace, C Smits, DL Vaux, J Silke, CL Day. (2010). Asymmetric Recruitment of cIAPs by TRAF2?.
      J Mol. Biol. 400, 8
Achieved using the OLIS DSM 10

L Dong, X Chen, Z Hu. (2007). Study of the effect of Cal-Red on the secondary structure of human serum albumin by spectroscopic techniques.
      J Molecular Struct. 846, 112-118
Achieved using the OLIS DSM 1000

M Itaya, IC Brett, SO Smith. (2012). Synthesis, Purification, and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy.
      Methods in Molecular Biology 831, 333-357
Achieved using the OLIS DSM 1000

V Srinivasan, C Rajendran, FL Sousa, AMP Melo, LM Saraiva, MM Pereira, M Santana, M Teixeira, H Michel. (2005). Structure at 1.3?? Resolution of?Rhodothermus marinus caa3?Cytochrome?c?Domain.
      J Mol. Biol. 345, 1047-1057
Achieved using the OLIS DW 2

W Zhang, J Li, L-W Liu, K-R Wang, J-J Song, J-X Yan, Z-Y Li, B-Z Zhang. (2010). A novel analog of antimicrobial peptide Polybia-MPI, with thioamide bond substitution, exhibits increased therapeutic efficacy against cancer and diminished toxicity in mice.
      Peptides 31, 1832-1838
Achieved using the OLIS DSM 1000

T Dworeck, A-K Petri, N Muhammad, M Fioroni, U Schwanberg. (2010). FhuA deletion variant ?1-159 overexpression in inclusion bodies and refolding with Polyethylene-Poly(ethylene glycol) diblock copolymer.
      Protein Expression and Purification 77, 75-79
Achieved using the OLIS DSM 17

AA Gilep, OL Guryev, SA Usanov, RW Estabrook. (2002). Apo-cytochrome b5?as an indicator of changes in heme accessability:: Preliminary studies with cytochrome P450 3A4.
      J Inorganic Biochemistry 87, 237-244
Achieved using the OLIS DW2

C Chang, V Simplaceanu, C Ho. (2002). Effects of Amino Acid Substitutions at ?131 on the Structure and Properties of Hemoglobin:? Evidence for Communication between ?1?1- and ?1?2-Subunit Interfaces.
      Biochemistry 41, 5644-5655
Achieved using the OLIS Olis SF

AM Sydor, H Lebrette, R Ariyakumaran, C Cavazza, DB Zamble. (2014). Relationship between Ni(II) and Zn(II) coordination and nucleotide binding by the Helicobacter pylori [NiFe]-hydrogenase and urease maturation factor HypB.
      Journal of Biological Chemistry 289, 3828-3841
Achieved using the OLIS RSM 1000

AF Mehl, UG Nalin, Z Ahmed, A Wells, TD Spyratos. (2011). Probing dimer interface stabilization within a four-helix bundle of the GrpE protein from?Escherichia coli?via internal deletion mutants: Conversion of a dimer to monomer.
      Int. J. of Biol. Macromolecules 48, 627-633
Achieved using the OLIS DSM 1000

H Sharifi, VK Walker, JA Ripmeester, P Englezos. (2014). Insights into the behaviour of biological clathrate hydrate inhibitors in aqueous saline solutions.
      Crystal Growth and Design 14, 2923-2930
Achieved using the OLIS DSM 1000

SS Wasko, G Tay, A Schwaighofer, C Nowak, JH Waite, A Miserez. (2013). Structural Proteins from Whelk Egg Capsule with Long Range Elasticity Associated with a Solid-state Phase Transition.
      Biomacromolecules 15, 30-42
Achieved using the OLIS DSM 1000

N Jawanda, J Ebalunode, A Gribenko, J Briggs, JC Lee, S -C Tu. (2008). A single-residue mutation destabilizes Vibrio harveyi flavin reductase FRP dimer.
      ABB 472, 51-57
Achieved using the OLIS DSM 1000

AA Fuller, FJ Seidl, PA Bruno, MA Plescia, KS Palla. (2011). Use of the environmentally sensitive fluorophore 4-N,N-dimethylamino-1,8-naphthalimide to study peptoid helix structures.
      Biopolymers Peptide Science 96, 627-638
Achieved using the OLIS DSM 1000

TDR Vance, LLC Olijve, RL Campbell, IK Voets, PL Davies, S Guo. (2014). Ca2+-stabilized adhesion helps an Antarctic bacterium reach out and bind ice.
      Bioscience Reports BSR20140083
Achieved using the OLIS GlobalWorks

TQ Faria, ZL de Almeida, PC Cruz, CSH Jesus, P Castanheira, RMM Brito. (2015). A look into amyloid formation by transthyretin: aggregation pathway and a novel kinetic model.
      Physical Chemistry Chemical Physics 17, 7255-7263
Achieved using the OLIS DSM 20

AJ van Hell, A Klymchenko, PP Burgers, EE Moret, W Jiskoot, We Hennink, DJA Crommelin, E Mastrobattista. (2010). Conformation and Intermolecular Interactions of SA2 Peptides Self-Assembled into Vesicles.
      J Phys B 114, 11046-11052
Achieved using the OLIS DSM 1000

J Oh, JJ Bowling, Y Zou, AG Chittiboyina, RJ Doerksen, D Ferreira, TD Leininger, MJ Hamann. (2013). Configurational assignments of conformationally restricted bis-monoterpene hydroquinones: Utility in exploration of endangered plants.
      Biochim Biophys Acta 1830, 4229-4234
Achieved using the OLIS DSM 20

PJ Buske and PA Levin. (2012). Extreme C Terminus of Bacterial Cytoskeletal Protein FtsZ Plays Fundamental Role in Assembly Independent of Modulatory Proteins.
      J. Biol. Chem. 287, 10945-10957
Achieved using the OLIS DM 45

L Wang and W Colon. (2007). Effect of Zinc, Copper, and Calcium on the Structure and Stability of Serum Amyloid A.
      Biochemistry 46, 5562-5569
Achieved using the OLIS DSM 10

JH Ippel, CJC de Haas, A Bunschoten, JAG Strijp, JAW Kruijtzer, RMJ Liskamp, J Kemmink. (2009). Structure of the Tyrosine-sulfated C5a Receptor N Terminus in Complex with Chemotaxis Inhibitory Protein of Staphylococcus aureus .
      J Biol Chem 284, 12363-12372
Achieved using the OLIS RSM 1000

AA Fuller, BA Yurash, EN Schaumann, FJ Seidl. (2013). Self-Association of Water-Soluble Peptoids Comprising (S)-N-1-(Naphylethyl)glycine Residues.
      Organic Letters 15, 5118-5121
Achieved using the OLIS DSM 1000

SW Pedersen, SB Pedersen, L Anker, G Hultqvist, AS Kristensen, P Jemth, K Stromgaard. (2014). Probing backbone hydrogen bonding in PDZ/ligand interactions by protein amide-to-ester mutations.
      Nature Communications 5, 3215
Achieved using the OLIS DSM 10

SM Lystved, S Volden, G Singh, M Yasuda, O Halskau. (2013). Tunable photophysical properties, conformation and function of nanosized protein-gold constructs..
      RSC Advances 3, 482-495
Achieved using the OLIS DSM 1000

S Ahmed, T Sprules, K Kaur. (2014). Structural Similarity between ?3-Peptides Synthesized from ??3-Homo-amino Acids or L-Aspartic Acid Monomers.
      Peptide Science 101, 359-367
Achieved using the OLIS DSM 17

YP Tan, UH Yildiz, W Wei, JH Waite, A Miserez. (2013). Layer-by-layer polyelectrolyte deposition: a mechanism for forming biocomposite materials.
      Biomacromolecules 14, 1715-1726
Achieved using the OLIS DSM 1000

N Bouxsein, L Jaeger, M Tirrell. (2010). Structural Responses of DNA-DDAB Films to Varying Hydration and Temperature .
      JACS 132, 7025-7035
Achieved using the OLIS DSM 1000

X Xu, VL Lockamy, K Chen, Z Huang, H Shields, SB King, SK Ballas, JS Nichols, MT Gladwin, CT Noguchi, AN Schechter, DB Kim-Shapiro. (2002). Effects of Iron Nitrosylation on Sickle Cell Hemoglobin Solubility .
      J Biol Chem 277, 36787-36792
Achieved using the OLIS RSM 10000

H Shao, X Xu, M-AA Mastrangelo, N Jing, RG Cook, GB Legge, DJ Tweardy. (2004). Structural Requirements for Signal Transducer and Activator of Transcription 3 Binding to Phosphotyrosine Ligands Containing the YXXQ Motif .
      J Biol Chem 279, 18967-18973
Achieved using the OLIS DSM 1000

K Nienhaus, F Renzi, B Vallone, J Wiedenmann, GU Nienhaus. (2006). Chromophore-Protein Interactions in the Anthozoan Green Fluorescent Protein asFP499.
      Biophys J 91, 4210-4220
Achieved using the OLIS Cary 14

W Ma, G Li, J Wang, W Yang, Y Zhang, PS Conti, K Chen. (2014). In vivo NIRF imaging-guided delivery of a novel NGR?VEGI fusion protein for targeting tumor vasculature.
      Amino Acids 46, 2721-2732
Achieved using the OLIS Cary 14

A Bouchard, N Javanovic, W Jiskoot, E Mendes, G-J Witkamp, DJA Crommelin, GW Hofland. (2007). Lysozyme Particle Formation During Supercritical Fluid Drying: Particle Morphology and Molecular Integrity.
      J Supercritical Fluids 40, 293-307
Achieved using the OLIS DSM 1000

B Metz, W Jiskoot, D Mekkes, R Kingma, WE Hennink, DJA Crommelin, GFA Kersten. (2007). Quality Control of Routine, Experimental and Real-time Aged Diptheria Toxoids by in vitro Analytical Techniques.
      Vaccine 25, 39-40
Achieved using the OLIS DSM 1000

P Asuri, SS Bale, RC Pangule, DA Shah, RS Kane, JS Dordick. (2007). Structure, Function, and Stability of Enzymes Covalently Attached to Single-Walled Carbon Nanotubes.
      Langmuir 23, 12318-12321
Achieved using the OLIS DSM 10

F Philippart, M Arlt, S Gotzen, S-J Tenne, M Bocola, H-H Chen, L Zhu, U Schwaneberg, J Okuda. (2013). A Hybrid Ring-Opening Metathesis Polymerization Catalyst Based on an Engineered Variant of the ?-Barrel Protein FhuA.
      Chemistry: A European Journal 19, 13865-13871
Achieved using the OLIS DSM 17

RJ Bauer, MT Begley, MA Trakselis. (2012). Kinetics and Fidelity of Polymerization by DNA Polymerase III from Sulfolobus solfataricus.
      Biochemistry 51, 1996-2007
Achieved using the OLIS DSM 17

R Fagerlund, PL Ooi, SM Wilbanks. (2012). Soluble expression and purification of tumor suppressor WT1 and its zinc finger domain.
      Protein Expression and Purification 85, 165-172
Achieved using the OLIS DSM 10

S Qiu, G Li, Slu, B Huang, Z Feng, C Li. (2012). Chiral Sulfur Compounds Studied by Raman Optical Activity: tert-Butanesulfinamide and its Precursor tert-Butyl tert-Butanethiosulfinate.
      Chirality 24, 731-740
Achieved using the OLIS DSM 1000

F Zhang, O Sadavski, SJ Xin, GA Wolley. (2007). Stabilization of Folded Peptide and Protein Structures via Distance Matching with a Long, Rigid Cross-Linker.
      JACS 129, 14154-14155
Achieved using the OLIS DSM 1000

BG Diaz, T Moldoveanu, MJ Kulper, RL Campbell, PL Davies. (2004). Insertion Sequence 1 of Muscle-specific Calpain, p94, Acts as an Internal Propeptide .
      J Biol Chem 279, 27656-27666
Achieved using the OLIS DSM 1000

C Chen, H Duan, C Gao, M Liu, X Wu,Y Wei, X Zhang, Z Liu. (2014). Non-covalent modification of thrombolytic agent- nattokinase: Simultaneous improvement of fibrinolysis activity and enzymatic stability.
      RSC Advances 4, 27422-27429
Achieved using the OLIS DSM 1000

CR Smallwood, L Jordan, V Trinh, DW Schuerch, A Gala, M Hanson, Y Shipelskiy, A Majumdar, SMC Newton, PE Klebba. (2014). Concerted loop motion triggers induced fit of FepA to ferric enterobactin.
      Journal of General Physiology 144, 71-80
Achieved using the OLIS SLM 8000 SF

R Hussain, K Benning, T Javorfi, E Longo, TR Rudd, B Pullford, G Siligardi. (2015). CDApps: integrated software for experimental planning and data processing at beamline B23, Diamond Light Source.
      Journal of Synchrotron Radiation 22 (Pt 2), 465-468
Achieved using the OLIS GlobalWorks

NA Ramella, GR Schinella, ST Ferreira, ED Prieto, ME Vela, JL Rios, MA Tricerri, OJ Rimoldi. (2012). Human Apolipoprotein A-I Natural Variants: Molecular Mechanisms Underlying Amyloidogenic Propensity.
      PLoS ONE 7(8): e43755
Achieved using the OLIS SLM 4800

D Gu, Y Zhou, V Kallhoff, B Baban, JJ Tanner, DF Becker. (2004). Identification and Characterization of the DNA-binding Domain of the Multifunctional PutA Flavoenzyme .
      J Biol Chem 279, 31171-31176
Achieved using the OLIS DSM 1000

TR Brown, ML Drummond, S Barelier, AS Crutchfield, A Dinescu, KD Slavens, TR Cundari, ME Anderson. (2011). Aspartate 458 of human glutathione synthetase is important for cooperativity and active site structure.
      Biochem. Biophys. Res Comm. 411, 536-542
Achieved using the OLIS DSM 1000

Y Hou, TB Hansen, A Staby, SM Cramer. (2010). Effects of Urea Induced Protein Conformational Changes on Ion Exchange Chromatographic Behavior.
      J Chromatography A 1217, 7393-7400
Achieved using the OLIS DSM 1000

M Hamborg, L Jorgensen, AR Bojsen, D Christensen, C Foged. (2013). Protein Antigen Adsorption to the DDA/TDB Liposomal Adjuvant: Effect on Protein Structure, Stability, and Liposome Physicochemical Characteristics.
      Pharamceutical Research 30, 140-155
Achieved using the OLIS DSM 10

FJ Bartl, O Fritze, E Ritter, R Hermann, V Kuksa, E Palczewski, KP Hofman, OP Ernst. (2005). Partial Agonism in a G Protein-coupled Receptor: ROLE OF THE RETINAL RING STRUCTURE IN RHODOPSIN ACTIVATION .
      J Biol Chem 280, 34259-34267
Achieved using the OLIS RSM 1000

C Hiser, DA Mills, M Schall, S Ferguson-Miller. (2001). C-Terminal Truncation and Histidine-Tagging of Cytochrome c Oxidase Subunit II Reveals the Native Processing Site, Shows Involvement of the C-Terminus in Cytochrome c Binding, and Improves the Assay for Proton Pumping.
      Biochemistry 40, 1606-1615
Achieved using the OLIS RSM 1000 SF

J Madine, HA Davies, E Hughes, DA Middleton. (2013). Heparin promotes the rapid fibrillization of a non-amyloidogenic peptide.
      Biochemistry 52, 8984-8992
Achieved using the OLIS Synchrotron DSM 20

KM Pegg, EM Liu, AC George, AE LaCuran, CR Bethel, RA Bonomo, P Oelschaeger. (2014). Understanding the determinants of substrate specificity in IMP family metallo-?-lactamases: The importance of S262.
      Protein Science DOI:?23, 1451-1460
Achieved using the OLIS DSM 20

M Fano, M van de Weert, E Horn Moeller, N Aaby Kruse, S Frokjaer. (2011). Ionic strength-dependent denaturation of?Thermomyces lanuginosus?lipase induced by SDS.
      Arch. Biochem. Biophys 506, 92-98
Achieved using the OLIS DSM 10

CL Piscitelli, TE Angel, BW Bailey, P Hargrave, EA Dratz, CM Lawrence. (2006). Equilibrium between Metarhodopsin-I and Metarhodopsin-II Is Dependent on the Conformation of the Third Cytoplasmic Loop .
      J Biol Chem 281, 6813-6825
Achieved using the OLIS UV-3000

P van Rijn, M Tutus, C Kathrein, NC Mougin, H Park, C Hein, MP Schurings, A Boker. (2014). Ultra-Thin Self-Assembled Protein-Polymer Membranes: A New Pore Forming Strategy.
      Advanced Functional Materials 24, 6762-6770
Achieved using the OLIS DSM 17

R Feltham, B Bettjeman, R Budhidarmo, PD Mace, S Shirly, SM Condon, SK Chunduru, MA McKinlay, DL Vauk, J Silke, CL Day. (2011). SMAC-mimetics activate the E3 ligase activity of cIAP1 by promoting RING dimerisation.
      J. Biol. Chem 286, 17015-17028
Achieved using the OLIS DSM 10

L Shin, WJ Cho, JD Cook, TL Stemmler, BP Jena. (2010). Membrane Lipids Influence Protein Complex Assembly-Disassembly.
      JACS 132, 5596-5597
Achieved using the OLIS DSM 16

MR Razeghifard. (2004). On-column refolding of recombinant human interleukin-4 from inclusion bodies.
      Protein Expression and Purification 37, 180-186
Achieved using the OLIS DSM 1000

MDR Peralta, A Karza, A Ngo, C Sierra, KT Fong, NR Hayre, N Mirzee, KM Ravikumar, AJ Kluber, X Chen, G-Y Liu, MD Toney, RR Singh, DL Cox. (2015). Engineering Amyloid Fibrils from ?-Solenoid Proteins for Biomaterials Applications.
      ACS Nano 9, 449-463
Achieved using the OLIS DSM 20

AP Batista, AS Fernandes, RO Lour, J Steuber, MM Pereira. (2010). Energy conservation by?Rhodothermus marinus?respiratory complex I.
      BBA Bioenergetics 1797, 509-515
Achieved using the OLIS DW2

M Fedurco, J Augustynski, C Indiani, G Smulevich, M Antalik, M Bano, E Sedlak, MC Glascock, JH Dawson. (2004). The heme iron coordination of unfolded ferric and ferrous cytochrome?c?in neutral and acidic urea solutions. Spectroscopic and electrochemical studies.
      BBA Proteins and Proteomics 1703, 31-41
Achieved using the OLIS DSM 16

L Cai, E Balogh, M Gochin. (2009). Stable Extended Human Immunodeficiency Virus Type 1 gp41 Coiled Coil as an Effective Target in an Assay for High-Affinity Fusion Inhibitors.
      Antimicrob. Agents Chemother 53, 2444-2449
Achieved using the OLIS DSM 20

M Gochin and L Cai. (2009). The Role of Amphiphilicity and Negative Charge in Glycoprotein 41 Interactions in the Hydrophobic Pocket.
      J Med Chem 52, 4338-4344
Achieved using the OLIS DSM 20

K Jiang, Q-Y Song, S-J Peng, Q-Q Zhao, G-D Li, Y Li, K Guo. (2015). New lignans from the roots of?Schisandra sphenanthera.
      Fitotera DOI: 10.1016/j.fitote.2015.03.015
Achieved using the OLIS DSM 1000

D Andrews, NR Mattatall, D Arnold, BC Hill. (2005). Expression, purification, and characterization of the CuA?cytochrome?c?domain from subunit II of the?Bacillus subtilis?cytochrome?caa3?complex in?Escherichia coli.
      Protein Expression and Purification 42, 227-235
Achieved using the OLIS DSM 1000

R Bitton, J Schmidt, M Biesalski, R Tu, M Tivrell, H Bianco-Peled. (2005). Self-Assembly of Model DNA-Binding Peptide Amphiphiles.
      Langmuir 21, 11888-11895
Achieved using the OLIS DSM 1000

K Zhu, A Jutila, EKJ Tuominen, SA Patkar, A Svendsen, PKJ Kinnunen. (2001). Impact of the tryptophan residues of?Humicola lanuginosa?lipase on its thermal stability.
      BBA Protein Structure and Molecular Enzymology 1547, 329-338
Achieved using the OLIS RSM 1000

Q Wang, Y Zhang, H Sun, H Chen, X Chen. (2010). Study on the interaction of butyl?p-hydroxybenzoate with human serum albumin by molecular modeling and multi-spectroscopic method.
      J Luminescence 131, 206-211
Achieved using the OLIS DSM 1000

J Chen, Y Li, L-Q Yang, Y-Z Li, Z-B Nan, K Gao. (2011). Biological activities of flavonoids from pathogenic-infected Astragalus adsurgens.
      Food Chem. 131, 546-551
Achieved using the OLIS DSM 1000

DS Hwang and JH Waite. (2012). Three intrinsically unstructured adhesive proteins, mfp-1, mfp-2, and mfp-3: Analysis by circular dichroism?.
      Protein Science 21, 1689-1695
Achieved using the OLIS DSM 1000

Y Li, L St. Louis, BI Knapp, D Muthu, B Anglin, D Giuvelis, JM Bidlack, EJ Bilsky, R Polt. (2014). Can Amphipathic Helices Influence the CNS Antinociceptive Activity of Glycopeptides Related to ?-Endorphin?.
      Journal of Medicinal Chemistry 57, 2237-2246
Achieved using the OLIS DSM 20

SA Cochrane and JC Vederas. (2014). Unacylated tridecaptin A1?acts as an effective sensitiser of Gram-negative bacteria to other antibiotics.
      International Journal of Antimicrobial Agents 44, 493-499
Achieved using the OLIS DSM 17

J Sugihara, L Sun, N Yan, HR Kaback. (2012). Dynamics of the?L-fucose/H+?symporter revealed by fluorescence spectroscopy.
      ?Proc. Natl. Acad. Sci. 109, 14847-14851
Achieved using the OLIS SLM 8000

DJ Adams, D Atkins, AI Cooper, S Furzeland, A Trewin, I Young. (2008). Vesicles from Peptidic Side Chain Polymers Synthesized by Atom Transfer Radical Polymerization.
      Biomacromolecules 9, 2997-3003
Achieved using the OLIS DSM 20

AD Nielsen, ML Pusey, CC Fuglsang, P Westh. (2003). A proposed mechanism for the thermal denaturation of a recombinant?Bacillus halmapalus??-amylase?the effect of calcium ions.
      BBA Proteins and Proteomics 1652, 52-63
Achieved using the OLIS DSM 1000

AL Fishburn, JR Keefe, AV Lissovnov, DH Peyton, SJ Anthony-Cahill. (2002). A Circularly Permuted Myoglobin Possesses a Folded Structure and Ligand Binding Similar to Those of the Wild-Type Protein but with a Reduced Thermodynamic Stability.
      Biochemistry 41, 13318-13327
Achieved using the OLIS DSM 10

E Maklashina, AB Kotlyar, G Cecchini. (2003). Active/de-active transition of respiratory complex I in bacteria, fungi, and animals.
      BBA Bioenergetics 1606, 95-103
Achieved using the OLIS RSM 1000

MD Hoos. (2009). Inhibition of Amyloid Beta-Protein Fibrillogenesis by Myelin Basic Protein.
      Ph.D. Thesis Stony Brook University
Achieved using the OLIS DSM 1000

WM Schluchter. (2011). Attachment of Non-cognate Chromophores to CpcA of Synechocystis sp. strain PCC 6803 expressed in E. coli.
      Biological Sciences Faculty Publications. Paper 2.: 4890 -4902
Achieved using the OLIS SLM 8000

G Thakur, K Prashanthi, T Thundat. (2013). Directed self-assembly of proteins into discrete radial patterns.
      Scientific Reports 3, 1923
Achieved using the OLIS DSM 17

TJ Sontag and CA Reardon. (2014). Polymorphisms of Mouse Apolipoprotein A-II Alter Its Physical and Functional Nature.
      PLoS ONE 9(2): e88705
Achieved using the OLIS GlobalWorks

DF Moriarty, C Fiorillo, C Miller, W colon. (2007). A truncated peptide model of the mutant P61A FIS forms a stable dimer.
      BBA Proteins and Proteomics 1774, 78-85
Achieved using the OLIS DSM 10

JD Wen and DM Gray. (2004). Ff Gene 5 Single-Stranded DNA-Binding Protein Assembles on Nucleotides Constrained by a DNA Hairpin.
      Biochemistry 43, 2622-2634
Achieved using the OLIS Cary 14

KH Ruan, V Cervantes, J Wu. (2009). Ligand-Specific Conformation Determines Agonist Activation and Antagonist Blockade in Purified Human Thromboxane A2 Receptor.
      Biochemistry 48, 3157-3165
Achieved using the OLIS DSM 1000

MA Corral-Rodriguez, PE Bock, E Hernandez-Carvajal, R Gutierrez-Gallego, P Fuentes-Prior. (2011). Structural basis of thrombin?mediated factor V activation: the Glu666?Glu672?sequence is critical for processing at the heavy chain?B domain junction.
      Blood 117, 7164-7173
Achieved using the OLIS SLM 8000

K Teilum, K Maki, BB Kragelund, FM Poulsen, H Roder. (2002). Early kinetic intermediate in the folding of acyl-CoA binding protein detected by fluorescence labeling and ultrarapid mixing .
      PNAS 99, 9807-9812
Achieved using the OLIS Titrator

RW Noble, HL Hui, CD Kwiatkowski, P Paily, A DeYoung, A Wierzba, JE Colby, S Bruno, A Mozzarelli. (2001). Mutational Effects at the Subunit Interfaces of Human Hemoglobin:? Evidence for a Unique Sensitivity of the T Quaternary State to Changes in the Hinge Region of the ?1?2 Interface.
      Biochemistry 40, 12357-12368
Achieved using the OLIS RSM 1000 SF

HNH Wilks, TM Arrington, BM Britt. (2014). Volume Change of the Random Coil to Folded Conformational Transition of Thermomyces lanuginosus Xylanase at 24oC and pH=7 via Application of the Clausius-Clapeyron Equation.
      Journal of Biophysical Chemistry 5, 134-142
Achieved using the OLIS DSM 1000

G Bilofsky, M Kolaczkowski, E Admas, J Schreibert, V Eisenberg, CM Coleman. (2013). Fungal ABC Transporter-Associated Activity of Isoflavonoids from the Root Extract of Dalea formosa.
      J. Natural Products 76, 915-925
Achieved using the OLIS DSM 20

M Noronha, JC Lima, E Paci, H Santos, AL Macanita. (2007). Tracking Local Conformational Changes of Ribonuclease A Using Picosecond Time-Resolved Fluorescence of the Six Tyrosine Residues.
      Biophys J 92, 4401-4414
Achieved using the OLIS DW2

JD Cook, WJ Cho, TL Stemmler, BP Jena. (2008). Circular dichroism (CD) spectroscopy of the assembly and disassembly of SNAREs: The proteins involved in membrane fusion in cells.
      Chem. Phys. Lett. 462, 6-9
Achieved using the OLIS DSM 16

E Wang, WL Erdahl, SA Hamidinia, CJ Chapman, RW Taylor, DR Pfeiffer. (2001). Transport Properties of the Calcium Ionophore ETH-129.
      Biophys. J 81, 3275-3284
Achieved using the OLIS DW2

J Litt, C Padala, P Asuri, S Vuntukuru, K Athmakuri, S Kumar, J Dordick, RS Kane. (2009). Enhancing Protein Stability by Adsorption onto Raftlike Lipid Domains.
      JACS 131, 7107-7111
Achieved using the OLIS DSM 10

S Hermeling, L Aranha, JMA Damen, M Slijper, H Schellekens, DJA Crommelin, W Jiskoot. (2005). Structural Characterization and Immunogenicity in Wild-Type and Immune Tolerant Mice of Degraded Recombinant Human Interferon Alpha2b.
      Pharm. Res. 12, 1997-2006
Achieved using the OLIS DSM 1000

Y Li, T-T Wang, J Zhao, X Shi, S-C Hu, K Gao. (2012). Norditerpenoids from Agathis macrophylla.
      Food Chemistry 131, 972-976
Achieved using the OLIS DSM 1000

JM Jungles, MP Dukes, N Vunnam, S Pedigo. (2014). Impact of pH on the Structure and Function of Neural Cadherin.
      Biochemistry 53, 7436-7444
Achieved using the OLIS DSM 20

AS Fraga, AC Esteves, N Micaelo, PF Cruz, RMM Brito, M Nutley, A Cooper, MMT Barros, EMV Pires. (2012). Functional and conformational changes in the aspartic protease cardosin A induced by TFE.
      Int. J. Biological Macromolecules 50, 323-330
Achieved using the OLIS DSM 20

VK Garripelli and S Jo. (2012). Nanocomposite thermogel for controlled release of small proteins.
      Journal of Bioactive and Compatible Polymers 27, 198-209
Achieved using the OLIS DSM 20

SP Balashov, ES Imasheva, JM Wang, JK Lanyi. (2008). Excitation energy transfer and the relative orientation of retinal and carotenoid in xanthorhodopsin.
      Biophys J 95, 2402-2414
Achieved using the OLIS SLM 8000

L Zhong, RS Tu, ML Gilchrist. (2013). Tether-Supported Biomembranes with Alpha-Helical Peptide Based Anchoring Constructs.
      Langmuir 29, 299-307
Achieved using the OLIS DSM 20

R Hussain, T Javorfi, G Siligardi. (2012). Circular dichroism beamline B23 at the Diamond Light Source.
      J. Synchrotron Rad. 19, 132-135
Achieved using the OLIS Custom CD

JA Kyndt, SN Sawides, S Memmi, M Koh, JC Fitch, TE Meyer, MP Heyn, JJ Van Beeumen, MA Cusanovich. (2007). Structural Role of Tyrosine 98 in Photoactive Yellow Protein: Effects on Fluorescence, Gateway, and Photocycle Recovery.
      Biochemistry 46, 95-105
Achieved using the OLIS GlobalWorks

AJ Middleton, AM Brown, PL Davies, VK Walker. (2009). Identification of the ice-binding face of a plant antifreeze protein?.
      FEBS Lett. 583, 815-819
Achieved using the OLIS DSM 1000

S Ahmed, R Beleld, T Sprules, K Kaur. (2007). Solid-Phase Synthesis and CD Spectroscopic Investigations of Novel ?-Peptides from l-Aspartic Acid and ?-Amino-l-alanine.
      Org Letters 9, 25-28
Achieved using the OLIS DSM 17

RE Berry, TK Shokhireva, I Filippov, MN Shokirev, H Zhang, FA Walker. (2007). Effect of the N-Terminus on Heme Cavity Structure, Ligand Equilibrium, Rate Constants, and Reduction Potentials of Nitrophorin 2 from Rhodnius prolixus.
      Biochemistry 46, 6830-6843
Achieved using the OLIS RSM 1000 SF

Z Fu, D Aucoin, J Davis, WE Van Nostrand. (2015). Mechanism of nucleated conformational conversion of A?42.
      Biochemistry DOI:?10.1021/acs.biochem.5b00467
Achieved using the OLIS DSM 1000

S-J Tenne and U Scheaneberg. (2012). First Insights on Organic Cosolvent Effects on FhuA Wildtype and Fhu (Delta)1-159.
      Int. Mol. Sci. 13, 2459-2471
Achieved using the OLIS DSM 17

I Smirnova, V Kasho, J Sugihara, HR Kaback. (2009). Probing of the rates of alternating access in LacY with Trp fluorescence .
      PNAS 106, 21561-21566
Achieved using the OLIS SLM 8100

NB Gillespie, L Ren, L Ramos, H Daniell, D Dews, KA Utzat, JA Stuart, CH Buck, RR Birge. (2005). Characterization and Photochemistry of 13-Desmethyl Bacteriorhodopsin.
      J. Phys. Chem. B 109, 16142-16152
Achieved using the OLIS RSM 1000

M Krewinkel, T Deworeck, M Fioroni. (2011). Engineering of an E. coli Outer Membrane Protein FhuA with Increased Channel Diameter.
      Journal of Nanobiotechnology 9, 33
Achieved using the OLIS DSM 1000

JS de Melo, G Quintero, J Pina, S Breda, R Fausto. (2001). Spectroscopic characterization of ?- and ?-pyrones and their substituted 4-hydroxy and 4-methoxy derivatives: an integrated infrared, photophysical and theoretical study.
      J Molecular Biology 565, 59-67
Achieved using the OLIS Cary 14

BD Reilly. (2006). Structural comparison of human C4A3 and C4B1 after proteolytic activation by C1s.
      Molecular Immunology 43, 800-811
Achieved using the OLIS DSM 10

JL Klassen, R McKay, JM Foght. (2009). 2?-Methyl and 1?-xylosyl derivatives of 2?-hydroxyflexixanthin are major carotenoids of Hymenobacter species?.
      Tetrahedron Letters 50, 2656-2660
Achieved using the OLIS DSM 17

ML van Slooten, AGWG Visser, A van Hoek, G Storm, DJA Crommelin, W Jiskoot. (2000). Conformational Stability of Human Interferon-Gamma on Association with and Dissociation from Liposomes.
      J Pharm. Sciences. 89, 1605-1629
Achieved using the OLIS DSM 1000

ME Wiltrout, JL Giovannelli, V Simplaceanu, JA Lukin, NT Ho, C Ho. (2005). A Biophysical Investigation of Recombinant Hemoglobins with Aromatic B10 Mutations in the Distal Heme Pockets,.
      Biochemistry 44, 7207-7217
Achieved using the OLIS Stopped flow

AJ Martinolich, G Park, M Nakamotot, RE Gate, KE Wheeler. (2012). Structural and functional effects of Cu metalloprotein-driven silver nanoparticle dissolution.
      Environ. Sci. Tech. 46, 6355-6362
Achieved using the OLIS DSM 1000

J Zhou, C Wei, G Jia, X Wang, Z Feng, C Li. (2009). Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kineticsstar, open.
      Biochimie 91, 1104-1111
Achieved using the OLIS DSM 1000

A Sinaga, TA Hattan, KC Tan. (2007). Poly(acrylic acid)-block-poly(L-Valine): Evaluation of beta Sheet Formation and its Stability Using Circular Dichroism Techniques.
      Biomacromolecules 8, 2801-2808
Achieved using the OLIS RSM 1000

DS Majumdar, I Smirnova, V Kasho, E Nir, X Kong, S Weiss, HR Kaback. (2007). Single-molecule Chemistry and Biology Special Feature: Single-molecule FRET reveals sugar-induced conformational dynamics in LacY .
      PNAS 104, 12640-12645
Achieved using the OLIS SLM 8100

T Merbitz-Zahradnik, K Zwicker, JH Nett, TA Link, BL Trumpower. (2003). Elimination of the Disulfide Bridge in the Rieske Iron?Sulfur Protein Allows Assembly of the [2Fe-2S] Cluster into the Rieske Protein but Damages the Ubiquinol Oxidation Site in the Cytochrome bc1 Complex.
      Biochemistry 42, 13637-13645
Achieved using the OLIS DW2

M Noronha, JC Lima, M Bastos, H Santos, A Macanita. (2004). Unfolding of Ubiquitin Studied by Picosecond Time-Resolved Fluorescence of the Tyrosine Residue.
      Biophys J 87, 2609-2620
Achieved using the OLIS Cary 15

S Jayakanthan, SA Roberts, A Weichsel, JM Arguello, MM McEvoy. (2012). Conformations of the apo, substrate-bound and phosphate-bound ATP-binding domain of the Cu(II) ATPase CopB illustrate coupling of domain movement to the catalytic cycle.
      Bioscience Reports 32, 443-453
Achieved using the OLIS DSM 20

AF Chu-Kung, R Nguyen, KN Bozzelli, M Tirrell. (2010). Chain length dependence of antimicrobial peptide?fatty acid conjugate activity.
      J. Colloid and Interface Sci 345, 160-167
Achieved using the OLIS DSM 1000

G Siligardi, R Hussain, D Myatt, T Javorfi. (2010). B23 Circular Dichroism Beamline at the Diamond Light Source.
      Diamond Light Source Proceedings 1, e104, 1-3
Achieved using the OLIS DSM 20

KJ Lauersen, A Brown, A Middleton, PL Davies, VK Walker. (2011). Expression and characterization of an antifreeze protein from the perennial rye grass, Lolium perenne.
      Cryobiology 17, 10039-10049
Achieved using the OLIS DSM 1000

CA Olsen, G Bonke, L Vedal, A Adersen. (2007). ?-Peptide/?-Peptoid Chimeras.
      Org. Letters 9, 1549-1552
Achieved using the OLIS DSM 10

A Moawad, M Hetta, JK Zjawiony, D Ferreira, M Hifnawy. (2013). Two new dihydroamentoflavone glycosides from Cycas revoluta.
      Natural Products Research DOI: 10.1080/14786419.2013.832675
Achieved using the OLIS DSM 20

D Li, J Zhu, J Jin, X Yao. (2007). Studies on the binding of nevadensin to human serum albumin by molecular spectroscopy and modeling.
      J Molecular Struct. 846, 1-3
Achieved using the OLIS DSM 1000

J Mendez, A Monteagudo, KH Griebenow. (2012). Stimulus-responsive Controlled Release System by Covalent Immobilization of an Enzyme into Mesoporous Silica Nanoparticles.
      Bioconjugate Chem. 23, 698-704
Achieved using the OLIS DSM 10

S Dobney, D Chiasson, P Lam, SP Smith, WA Snedden. (2009). The Calmodulin-related Calcium Sensor CML42 Plays a Role in Trichome Branching .
      J Biol Chem 284, 31647-31657
Achieved using the OLIS DSM 1000

C Feng, HL Wilson, G Tollin, AV Astashkin, JT Hazzard, KV Rajagopalan, JH Enemark. (2005). The Pathogenic Human Sulfite Oxidase Mutants G473D and A208D are Defective in Intramolecular Election Transfer.
      Biochemistry 44, 13734-13743
Achieved using the OLIS RSM 1000

TP Curran and EL Handy. (2009). A 310-helix single turn enforced by crosslinking of lysines with 1,1?-ferrocenedicarboxylic acid.
      J Organometallic Chem 694, 902-907
Achieved using the OLIS DSM 16

IF Szylowska, J Nowacki, J Herbich. (2010). Structural changes accompanying excited-state electron transfer in meta- and para-dialkylaminopyridines.
      J PhotoChem. Photobiol. A Chem 209, 135-146
Achieved using the OLIS DSM 17

JJ Warren and JM Mayer. (2010). Predicting organic hydrogen atom transfer rate constants using the Marcus cross relation .
      PNAS 107, 5282-5287
Achieved using the OLIS RSM 1000

P Li, A Tan, CA Prestidge, HM Nielsen, A Mullertz. (2014). Self-nanoemulsifying drug delivery systems for oral insulin delivery:?In vitro?and?in vivo?evaluations of enteric coating and drug loading.
      International Journal of Pharmaceutics 477, 390-398
Achieved using the OLIS DSM 10

AR Lim, BP Sishta, Ag Mauk. (2006). Contribution of the heme propionate groups to the electron transfer and electrostatic properties of myoglobin.
      J Inorg. Biocheem. 100, 2017-2023
Achieved using the OLIS Durrum-Gibson

KW Bender, DM Rosenbaum, B Vanderbeld, M Ubaid, WA Snedden. (2013). The Arabidopsis calmodulin-like protein, CML39, functions during early seedling establishment.
      The Plant Journal 76, 634-647
Achieved using the OLIS GlobalWorks

A Sabahi and P Wittung-Stafshede. (2002). Unfolding the unique?c-type heme protein,?Chlamydomonas reinhardtii?cytochromef.
      BBA Protein Structure and Molecular Enzymology 1596, 163-171
Achieved using the OLIS DSM 1000

C Beltran, E Rodriquez-Miranda, G Granados-Gonzalez, LG de la Torre, T Nishigaki, A Darszon. (2014). Zn2+?induces hyperpolarization by activation of a K+?channel and increases intracellular Ca2+?and pH in sea urchin spermatozoa.
      Developmental Biology 394, 15-23
Achieved using the OLIS SLM 8000

IG Shabalina, M Ost, N Petrovic, M Vrbacky, J Nedergaard. (2010). Uncoupling protein-1 is not leaky?.
      BBA Bioenergetics 1797, 773
Achieved using the OLIS DW2

MS Freon, MV Frassa, M Ceolin, S Ituarte, J-W Qiu, J Sun, PE Fernandez, H Heras. (2013). Novel Animal Defenses against Predation: A Snail Egg Neurotoxin Combining Lectin and Pore-Forming Chains That Resembles Plant Defense and Bacteria Attack Toxins.
      PLosOne 8(5): e63782
Achieved using the OLIS SLM 4800

JD Wen and DM Gray. (2002). The Ff Gene 5 Single-Stranded DNA-Binding Protein Binds to the Transiently Folded Form of an Intramolecular G-Quadruplex.
      Biochemistry 41, 11438-11448
Achieved using the OLIS Cary 14

DM Skytte, K Frydenvang, L Hansen, PG Nielsen, JW Jaroszewski. (2010). Synthesis and Characterization of an Epimer of Tacrolimus, an Immunosuppressive Drug.
      J Nat Prod 73, 776-779
Achieved using the OLIS DSM 10

AA Fuller, CA Holmes, FJ Seidl. (2013). A fluorescent peptoid pH-sensor.
      Biopolymers 100, 380-386
Achieved using the OLIS DSM 1000

VR Sharma and RD Sheardy. (2014). The Human Telomere Sequence, (TTAGGG)4, in the Absence and Presence of Consulates: A Spectroscopic Investigation.
      Molecules 19, 595-601
Achieved using the OLIS DSM 1000

M Zhong, Y-X Ma, J-X Liu, D-L Di. (2014). A new quaternary protoberberine alkaloid isolated from Dicranostigma leptopodum (Maxim) Fedde.
      Natural Products Research 28, 507-510
Achieved using the OLIS DSM 1000

JV Badami, P Desir, RS Tu. (2014). Integration of Surface-Active, Periodically Sequenced Peptides into Lipid-Based Microbubbles.
      Langmuir 30, 8839-8847
Achieved using the OLIS DSM 20

LH Moller, JS Bahnsen, HM Nielsen, J Ostergaard, S Sturup, B Gammelgaard. (2014). Selenium as an alternative peptide label-comparison to fluorophore-labelled penetratin.
      European Journal of Pharmaceutical Sciences 67, 76-84
Achieved using the OLIS DSM 10

IH Hanna, MS Kim, FP Guengerich. (2001). Heterologous Expression of Cytochrome P450 2D6 Mutants, Electron Transfer, and Catalysis of Bufuralol Hydroxylation: The Role of Aspartate 301 in Structural Integrity.
      Archives of Biochemistry and Biophysics 393, 255-261
Achieved using the OLIS DW2/Cary 14

CL Higgins, J Meyer, P Wittung-Stafshede. (2002). Exceptional stability of a [2Fe?2S] ferredoxin from hyperthermophilic bacteriumAquifex aeolicus.
      BBA Proteins and Proteomics 1599, 82-89
Achieved using the OLIS DSM 1000

SK Kim, A Rahman, RC Conover, MK Johnson, JT Mason, V Gomes, M Hirasawa, ML Moore, T Leustek, DB Knaff. (2006). Properties of the Cysteine Residues and the Iron-Sulfur Cluster of the Assimilatory 5'Adenylyl Sulfate Reductase from Enteromorpha intestinalis.
      Biochemistry 45, 5010-5018
Achieved using the OLIS DSM 10

FR Schumacher, G wilson, CL Day. (2013). The N-terminal extension of UBE2E ubiquitin conjugating enzymes limits chain assembly.
      J. Mol. Biol. 81, 1988-1996
Achieved using the OLIS DSM 10

JR Kesting, L Olsen, D Staerk, MV Tejesvi. KR Kini, HS Prakash, JW Jaroszewski. (2011). Production of Unusual Dispiro Metabolites in Pestalotiopsis virgatula Endophyte Cultures: HPLC-SPE-NMR, Electronic Circular Dichroism, and Time-Dependent Density-Functional Computation Study.
      J. Nat. Prod. 74, 2206-2215
Achieved using the OLIS DSM 10

X Qian, T Mester, L Morgado, T Arkawa, ML Sharma, K Inoue, C Joseph, CA Salgueiro, MJ Maroney, DR Lovley. (2011). Biochemical characterization of purified OmcS, a?c-type cytochrome required for insoluble Fe(III) reduction in?Geobacter sulfurreducens.
      BBA Bioenergetics 1807, 404-412
Achieved using the OLIS RSM 1000

S Farmanesh, S Ramamoorty, J Chung, JR Asplin, P Karande, JD Rimer. (2014). Specificity of Growth Inhibitors and their Cooperative Effects in Calcium Oxalate Monohydrate Crystallization.
      Journal of the American Chemical Society 136, 367-376
Achieved using the OLIS DSM 1000

GA Lengyel, ZE Reinert, BD Griffith, WS Horne. (2014). Comparison of Backbone Modification in Protein ?-Sheets by ??? Residue Replacement and ?-Residue Methylation.
      Organic and Biomedical Chemistry 12, 5375-5381
Achieved using the OLIS DSM 17

M Hospes, J Hendriks, KJ Hellingwerf. (2013). Tryptophan fluorescence as a reporter for structural changes in photoactive yellow protein elicited by photo-activation.
      Photochem. Photobiol. Sci. 12, 479-488
Achieved using the OLIS DM 45

KC Kondapalli, NM Kok, D Danius, TL Stemmler. (2008). Drosophila Frataxin: An Iron Chaperone during Cellular Fe?S Cluster Bioassembly.
      Biochemistry 47, 6917-1927
Achieved using the OLIS Cary 60 CD

WD Nes, P Jayasimha, W Zhou, R Kanagasabai, C Jin, TT Jaradat, RW Shaw, JM Bujnicki. (2004). Sterol methyltransferase: Functional Analysis of Highly Conserved Residues by Site Directed Mutagenesis.
      Biochemistry 43, 569-576
Achieved using the OLIS DSM 1000

ETJ van den Bremer, W Jiskoot, R James, GR Moore, C Kleanthous, AJR Heck, CS Maier. (2002). Probing Metal Ion Binding and Conformational Properties of the Colicin E9 Endonuclease by Electrospray Time of Flight Mass Spectroscopy.
      Prot. Sci. 11, 1738-1752
Achieved using the OLIS DSM 1000

MI de Jonge, MP Bos, HJ Hamstra, W Jiskoot, P can Ulsen, J Tommassen, L van Alphen, P van der Ley. (2002). Conformational Analysis of Opacity Proteins from Neisseria Meningitidis.
      Eur. J. Biochem. 269, 5215-5223
Achieved using the OLIS DSM 1000

L Ying, W Chao, L Guanghua. (2010). Interaction of Jatrorrhizine with Human Gamma Globulin in membrane mimetic environments: Probing of the binding mechanism and binding site by spectroscopic and molecular modeling methods?.
      J Mol Struct. 980, 108-113
Achieved using the OLIS DSM 1000

D Sung, ZW Chen, FS Mathews, VL Davidson. (2002). Mutation of ?Phe55 of Methylamine Dehydrogenase Alters the Reorganization Energy and Electronic Coupling for Its Electron Transfer Reaction with Amicyanin,.
      Biochemistry 41, 13926-13933
Achieved using the OLIS RSM 1000

M van de Weert, J Hoechstetter, WE Hennink, JA Crommelin. (2000). The Effect of a Water/Organic Solvent Interface on the Structural Stability of Lysozyme.
      J Controlled Release 68, 351-359
Achieved using the OLIS DSM 1000

M Elgeti, R Kazmin, M Heck, T Morizumi, E Ritter, P Sheerer, OP Ernst, F Siebert, KP Hofmann, FJ Bartl. (2011). Conserved Tyr2235.58?Plays Different Roles in the Activation and G-Protein Interaction of Rhodopsin.
      J. Amer. Chem. Soc. 133, 7159-7165
Achieved using the OLIS RSM 1000

J Babcock and L Brancaleon. (2013). Bovine Serum Albumin Oligomers in the E- and B-Forms at Low Protein Concentration and Ionic Strength.
      Int. J. Biolog. Macromol. 53, 42-53
Achieved using the OLIS DSM 17

KC Cunha, VH Rusu, IFT Viana, ETA Marques, R Dhalia, RD Lins. (2015). Assessing protein conformational sampling and structural stability via?de novo?design and molecular dynamics simulations.
      Biopolymers DOI:?10.1002/bip.22626
Achieved using the OLIS DSM 17

J Hwang and TJ Deming. (2001). Methylated Mono- and Di(ethylene glycol)-Functionalized ?-Sheet Forming Polypeptides.
      Biomacromolecules 2, 17-21
Achieved using the OLIS DSM 1000

AT Bender, Y Kamada, PA Kleveland, Y Osawa. (2002). Assembly and activation of heme-deficient neuronal NO synthase with various porphyrins.
      J Inorganic Biochemistry 91, 625-634
Achieved using the OLIS DW2

AM Garcia Costas, Y Tsukatani, SP Romberger, GT Oostergetel, EJ Boekema, JH Golbeck, DA Bryant. (2011). Ultrastructural Analysis and Identification of Envelope Proteins of ?Candidatus Chloracidobacterium thermophilum? Chlorosomes.
      J. Bacteriology 193, 6701-6711
Achieved using the OLIS SLM 8000

M Fernandez-Vidal, S Jayasinghe, AS Ladakhin, SH White. (2007). Folding Amphipathic Helices Into Membranes: Amphiphilicity Trumps Hydrophobicity.
      J Mol Biol 370, 459-470
Achieved using the OLIS SLM 8000

D Toubarro, MM Avila, YJ Hao, N Balasubramanian, Y Jing, R Montiel, TQ Faria, RM Brito, N Simoes. (2013). A Serpin Released by an Entomopathogen Impairs Clot Formation in Insect Defense System.
      PLoS ONE 8(7): e69161
Achieved using the OLIS DSM 20

V Cabra, E Vazquez-Contreras, a Moreno, R Arreguin-Espinosa. (2008). The effect of sulfhydryl groups and disulphide linkage in the thermal aggregation of Z19 ?-zein.
      BBA Proteins and Proteomics 1784, 1028-1036
Achieved using the OLIS DM 45

DC Lamb, Y Kim, LU Vermalitskaya, YN Yermalitsky, GI Lepesheva, SL Kelly, MR Waterman, LM Podust. (2006). A Second FMN Binding Site in Yeast NADPH-Cytochrome P450 Reductase Suggests a Mechanism of Electron Transfer by Diflavin Reductases.
      Structure 14, 51-61
Achieved using the OLIS RSM 1000

RS Phillips, EW Miles, G Holtermann, RS Goody. (2005). Hydrostatic Pressure Affects the Conformational Equilibrium of Salmonella typhimurium Tryptophan Synthase.
      Biochemistry 44, 7921-7928
Achieved using the OLIS Cary 14

H Lin, EN Kitova, MA Johnson, L Eugenio, KKS Ng, JS Klassen. (2012). Electrospray Ionization-Induced Protein Unfolding.
      Journal of the American Society for Mass Spectrometry 23, 2122-2131
Achieved using the OLIS DSM 17

S Azmi, K Jiang, M Stiles, T Thundat, K Kaur. (2015). Detection of Listeria monocytogenes with short peptide fragments from class IIa bacteriocins as recognition elements.
      ACS Combinatorial Science 17, 156-163
Achieved using the OLIS DSM 17

MN Perdersen, V Fodera, I Horvath, A van Maarschalkerweerd, KN Toft, C Weise, F Almqvist, M Wolf-Watz, P Wittung-Stafshede, B Versergaard. (2015). Direct Correlation Between Ligand-Induced ?-Synuclein Oligomers and Amyloid-like Fibril Growth.
      Scientific Reports 5, Article number: 10422
Achieved using the OLIS DSM 10

MJ Hatfield, S Gillespie, Y Chen, Z Li, CJ Cassady, JB Vincent. (2006). Low-molecular-weight chromium-binding substance from chicken liver and American alligator liver?.
      Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 144, 423-431
Achieved using the OLIS Cary 14

AJ Wyman, H Popelkova, CF Yocum. (2008). Site-Directed Mutagenesis of Conserved C-Terminal Tyrosine and Tryptophan Residues of Psbo, the Photosystem II Manganese-Stabalizing Protein, Alters Its Activities and Fluorescence Properties.
      Biochemistry 47, 6490-6498
Achieved using the OLIS Cary 17

DA Mills, L Floens, C Hiser, J Qian, S Ferguson-Miller. (2000). Where is ?outside? in cytochrome c oxidase and how and when do protons get there?.
      BBA Bioenergetics 1458, 180-187
Achieved using the OLIS RSM 1000

V Carvalho, P Castanheira, TQ Faria, C Goncalves, P Madureira, C Faro, L Domingues, RMM Brito, M Vilanova, M Gama. (2010). Biological activity of heterologous murine interleukin-10 and preliminary studies on the use of a dextrin nanogel as a delivery system.
      Int. J. Pharmaceutics 400, 234-242
Achieved using the OLIS DSM 20

M Ghosh, Y Wang, CE Ebert, S Vadlamuri, DS Beattie. (2001). Substituting Leucine for Alanine-86 in the Tether Region of the Iron?Sulfur Protein of the Cytochrome bc1 Complex Affects the Mobility of the [2Fe2S] Domain.
      Biochemistry 40, 327-335
Achieved using the OLIS Cary 17

C Wagner, W Buchegger, M Vellekoop, M Kraft, B Lendl. (2010). Time-resolved mid-IR spectroscopy of (bio)chemical reactions in solution utilizing a new generation of continuous-flow micro-mixer.
      Chemistry and Material Science 400, 2487-2497
Achieved using the OLIS IR Stopped Flow

Y Li, T Morgan, and SW Ragsdale. (2010). Identification of a Thiol/Disulfide Redox Switch in the Human BK Channel That Controls Its Affinity for Heme and CO.
      JBC 285, 20117-20127
Achieved using the OLIS Cary 14

R Budhidarmo and CL Day. (2014). The Ubiquitin-Associated Domain of Cellular Inhibitor of Apoptosis Proteins Facilitates Ubiquitylation.
      Journal of Biological Chemistry 289, 25721-25736
Achieved using the OLIS DSM 10

PN Achar, MY Sreenivasa, G Galdo. (2011). Comparative studies on the changes of total soluble proteins and protease activity in Georgian commercial peanuts contaminated by Aspergillus flavus.
      Archives of Phytopathology and Plant Protection 45, 220-227
Achieved using the OLIS DM 45

O Julien, S Chatterjee, TC Bjorndahl, B Sweeting, S Acharya, V Semenchenko, A Chakrabartty, EF Pai, DS Wishart, BD Sykes, NR Cashman. (2011). The relative and regional stabilities of the hamster, mouse, rabbit and bovine prion proteins towards urea unfolding assessed by NMR and CD spectroscopies..
      Biochemsitry 50, 7536-7545
Achieved using the OLIS DSM 17

MD Wysta, AL Cogen, TJ Deming. (2001). A Parallel Synthetic Approach for the Analysis of Membrane Interactive Copolypeptides.
      JACS 123, 12919-12920
Achieved using the OLIS DSM 1000

M Hijnen, PGM van Gageldonk, GAM Berbers, T van Woerkman, FR Mooi. (2005). The?Bordetella pertussis?virulence factor P.69 pertactin retains its immunological properties after overproduction in?Escherichia coli.
      Protein Expression and Purification 41, 106-112
Achieved using the OLIS DSM 1000

KP Conner, AM Schimf, AA Cruce, KJ McLean, AW Munro, DJ Frank, MD Krzyaniak, PR Ortiz de Montellano, MK Bowman, WM Atkins. (2014). Strength of Axial Water Ligation in Substrate-Free Cytochrome P450s is Isoform Dependent.
      Biochemistry 53, 1428-1434
Achieved using the OLIS DW-2

TD Mealman, M Zhou, T Affandi, KN Chacon, ME Aranguren, NJ Blackburn, VH Wysocki, MM McEvoy. (2012). The N-terminal region of CusB is sufficient for metal binding and metal transfer with the metallochaperone CusF.
      Biochemistry 51, 6767-6775
Achieved using the OLIS DSM 20

C Galicia, G Mendoza-Hernandez, A Rodriguez-Romero. (2015). Impact of the vulcanization process on the structural characteristics and IgE recognition of two allergens, Hev b 2 and Hev b 6.02, extracted from latex surgical gloves.
      Molecular Immunology 65, 250-258
Achieved using the OLIS DM 45

AH Ghassemi. (2011). Microspheres based on biodegradable functionalized poly(alpha-hydroxy) acids fro the controlled release of bioactive proteins and peptides.
      PhD thesis Utrecht Inistitute of Pharmaceutical Sciences
Achieved using the OLIS DSM 1000

RJ Debus, C Aznar, KA Campbell, W Gregor, BA Diner, RD Britt. (2003). Does Aspartate 170 of the D1 Polypeptide Ligate the Manganese Cluster in Photosystem II? An EPR and ESEEM Study.
      Biochemistry 42, 10600-10608
Achieved using the OLIS Cary 14

J Kortekaas, SA Muller, P Ringler, M Gregorini, VE Weynants, L Rutten, MP Bos, J Tommassen. (2006). Immunogenicity and structural characterisation of an in vitro folded meningococcal siderophore receptor (FrpB, FetA).
      Microbes and Infection 8, 2145-2153
Achieved using the OLIS DSM 1000

KV Eaton, WJ Anderson, MS Dubrava, VK Kumirov, EM Dykstra, MHJ Cordes. (2015). Studying protein fold evolution with hybrids of differently folded homologs.
      Protein Engineering Design and Selection DOI:?10.1093/protein/gzv027
Achieved using the OLIS DSM 20

AK Mahalka, T Kirkegaard, LTI Jukola, M Jaattela, PKJ Kinnunen. (2014). Human heat shock protein 70 (Hsp70) as a peripheral membrane protein.
      Biophysica et Biochimica Acta: Biomembranes 1838, 1344-1361
Achieved using the OLIS DSM 1000

AL Jacob-Ferreira, MY Kondo, PK Baral, MNG James, A Holt, X Fan, R Schultz. (2013). Phosphorylation Status of 72 kDa MMP-2 Determines Its Structure and Activity in Response to Peroxynitrite.
      PLoS ONE 8(8): e71794
Achieved using the OLIS DSM 17

KP Neupane and J Shearer. (2006). The Influence of Amine/Amide versus Bisamide Coordination in Nickel Superoxide Dismutase.
      Inorg. Chem. 45, 10552-10566
Achieved using the OLIS Cary 14/DSM 17

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