Publications from the Lab of Dr. Guengerich of Vanderbilt University

Guengerich, F.P., Persmark, M., and Humphreys, W.G. (1993) Chem. Res. Toxicol. 6, 635-648. "Formation of 1, N2- and N2,3-Ethenoguanine Derivatives from1-Halooxiranes: Isotopic Labeling Studies and Formation of a Hemiaminal Derivative of N2-(2-Oxoethyl)guanine."

Thier, R., Pemble, S.E., Taylor, J.B., Humphreys, W.G., Persmark, M.,Ketterer, B., and Guengerich, F.P. (1993) Proc. Natl. Acad. Sci. USA 90, 8576-8580. "Expression of Mammalian Glutathione S#030#Transferase 5-5 in Salmonella typhimurium TA1535 Leads to Base-pair Mutations Upon Exposure to Dihalomethanes."

Sandhu, P., Guo, Z., Baba, T., Martin, M.V., Tukey, R.H., and Guengerich, F.P.(1994) Arch. Biochem. Biophys. 309, 168-177. "Expression of Modified Human Cytochrome P450 1A2 in Escherichia coli: Stabilization, Purification, Spectral Characterization, and Catalytic Activities of the Enzyme."

Persmark, M., and Guengerich, F.P. (1994) Biochemistry 33, 8662-8672.

"Spectral and Thermodynamic Characterization of the Interaction of N7-Guanyl Thioether Derivatives of d(TGCTG*CAAG) with Potential Complements."

Yamazaki, H., Guo, Z., Persmark, M., Mimura, M., Gonzalez, F.J., Sugahara, C.,Guengerich, F.P., and Shimada, T. (1994) Mol. Pharmacol. 46, 568-577. "Bufuralol Hydroxylation by Cytochrome P450 2D6 and 1A2 Enzymes in Human Liver Microsomes."

Gillam, E.M.J., Guo, Z., Martin, M.V., Jenkins, C.M., and Guengerich, F.P. (1995) Arch. Biochem. Biophys. 319, 540-550. "Expression of Cytochrome P450 2D6 in Escherichia coli, Purification, and Spectral and Catalytic Characterization."

Yamazaki, H., Ueng, Y-F., Shimada, T., and Guengerich, F.P. (1995) Biochemistry 34, 8380-8389. "Roles of Divalent Metal Ions in Oxidations Catalyzed by Recombinant Cytochrome P450 3A4 and Replacement of NADPH-Cytochrome P450 Reductase with Other Flavoproteins, Ferredoxin, and Oxygen Surrogates."

Dong, M-S., Yamazaki, H., Guo, Z., and Guengerich, F.P. (1996) Arch. Biochem. Biophys. 327, 11-19. "Recombinant Human Cytochrome P450 1A2 and an N-Terminal Truncated Form: Construction, Purification, Aggregation Properties, and Interactions with Flavodoxin, Ferredoxin, and NADPH-Cytochrome P450 Reductase."

Chun, Y-J., Shimada, T., and Guengerich, F.P. (1996) Arch. Biochem. Biophys. 330, 48-58. "Construction of a Human Cytochrome P450 1A1:Rat NADPH-P450 Reductase Fusion Protein cDNA, Expression in Escherichia coli, Purification, and Catalytic Properties of the Enzyme in Bacterial Cells and After Purification."

Nakhgevany, R., Bonsu, O., Barber, J., Mabrouk, P.A., Ueng, Y-F., Bell, L.C., and Guengerich, F.P. (1996) Biochem. Biophys. Res. Commun. 222, 406-409. "Preparation, Purification, and Spectrophometric Characterization of Rabbit Cytochrome P450 1A2 Conjugated with Polyethylene Glycol Monomethyl Ether."

Lowe, L.G., and Guengerich, F.P. (1996) Biochemistry 35, 9840-9849. "Steady-state and Pre-Steady-state Kinetic Analysis of dNTP Insertion Opposite 8-Oxo-7,8-dihydroguanine by Escherichia coli Polymerases I exo- and II exo-." 12840

Johnson, W.W., Harris, T.M., and Guengerich, F.P. (1996) J. Am. Chem. Soc.118, 8213-8220. "Kinetics and Mechanism of Hydrolysis of Aflatoxin B1 exo-8,9-Oxide and Rearrangement of the Dihydrodiol."

Dong, M-S., Bell, L.C., Guo, Z., Phillips, D.R., Blair, I.A., and Guengerich,F.P. (1996) Biochemistry 35, 10031-10040. "Identification of Retained N-Formylmethionine in Bacterial Recombinant Cytochrome P450 Proteins with the N-Terminal Sequence MALLLAVFL...: Roles of Residues 3-5 in Retention and Membrane Topology."

Yamazaki, H., Johnson, W.W., Ueng, Y-F., Shimada, T., and Guengerich, F.P. (1996) J. Biol. Chem. 271, 27438-27444. "Lack of Electron Transfer from Cytochrome b5 in Stimulation of Catalytic Activities of Cytochrome P450 3A4: Characterization of a Reconstituted Cytochrome P450 3A4/NADPH-cytochrome P450 Reductase System and Studies with Apo-cytochrome b5." 12841

Guengerich, F.P., Yun, C-H., and Macdonald, TELL. (1996) J. Biol. Chem. 271, 27321-27329. "Evidence for a One-Electron Mechanism in N-Dealkylation of N,N-Dialkylanilines by Cytochrome P450 2B1. Kinetic Hydrogen Isotope Effects,Linear Free Energy Relationships with Biomimetic Models, Comparisons with Horseradish Peroxidase, and Studies with Oxygen Surrogates." 12847

Mancy, A., Dijols, S., Poli, S., Guengerich, F.P., and Mansuy, D. (1996) Biochemistry 35, 16205-16212. "Interaction of Sulfaphenazole Derivatives with Human Liver Cytochromes P450 2C: Molecular Origin of the Specific Inhibitory Effects of Sulfaphenazole on CYP 2C9 and Consequences for the Substrate Binding Topology."

Foroozesh, M., Primrose, G., Guo, Z., Bell, L.C., Guengerich, F.P., and Alworth, W.L. (1997) Chem. Res. Toxicol. 10, 91-102. "Propynylaryl Acetylenes as Mechanism-based Inhibitors of Cytochrome P450 1A1, 1A2, and 2B1 Enzymes."

Guengerich, F.P., Vaz, A.D.N., Raner, G.M., Pernecky, S.J., and Coon, M.J. (1997) Mol. Pharmacol. 51, 147-151. "Evidence for a Role of a Perferryl-Oxygen Complex, FeO3+, in N-Oxygenation of Amines by Cytochrome P450 Enzymes."

Ueng, Y-F., Kuwabara, T., Chun, Y-J., and Guengerich, F.P. (1997) Biochemistry 36, 370-381. "Cooperativity in Oxidations Catalyzed by Cytochrome P450 3A4."

Parikh, A., and Guengerich, F.P. (1997) Protein Express. Purif. 9, 346-354. "Expression, Purification, and Characterization of a Catalytically Active Human Cytochrome P450 1A2:NADPH-Cytochrome P450 Reductase Fusion Protein." 12855

Langouet, S., Muller, M., and Guengerich, F.P. (1997) Biochemistry 36, 6069-6079. "Misincorporation of dNTPs Opposite 1,N2-Ethenoguanine and 5,6,7,9-Tetrahydro-7-hydroxy-9-oxoimidazo[1,2-a]purine in Oligonucleotides by Escherichia coli Polymerases I exo- and II exo-, T7 Polymerase exo-, Human Immunodeficiency Virus-1 Reverse Transcriptase, and Rat Polymerase b."

Furge, L.L., and Guengerich, F.P. (1997) Biochemistry 36, 6475-6487. "Analysis of Nucleotide Insertion and Extension at 8-Oxo-7,8-dihydroguanine by Replicative T7 Polymerase exo- and Human Immunodeficiency Virus-1 Reverse Transcriptase Using Steady-state and Pre-Steady-state Kinetics."

Johnson, W.W., and Guengerich, F.P. (1997) Proc. Natl. Acad. Sci. USA 94, 6121-6125. "Reaction of Aflatoxin B1-8,9-exo-Epoxide with DNA: Kinetics of Covalent Binding and DNA-induced Hydrolysis."

Parikh, A., Gillam, E.M.J., and Guengerich, F.P. (1997) Nature Biotechnol. 15, 784-788. "Drug Metabolism by Escherichia coli Expressing Human Cytochromes P450."

Gillam, E.M.J., Wunsch, R.M., Ueng, Y-F., Shimada, T., Reilly, P.E.B., Kamataki, T., and Guengerich, F.P. (1997) Arch. Biochem. Biophys. 346, 81-90. "Expression of Cytochrome P450 3A7 in Escherichia coli: Effects of 5'Modification and Catalytic Characterization of Recombinant Enzymes Expressed in Bicistronic Format with NADPH-Cytochrome P450 Reductase." 12869

Shaw, P.M., Hosea, N.A., Thompson, D.V., Lenius, J.M., and Guengerich, F.P.(1997) Arch. Biochem. Biophys. 348, 107-115. "Reconstitution Premixes for Assays Using Recombinant Human Cytochrome P450, NADPH-Cytochrome P450 Reductase, and Cytochrome b5."

Kim, M-S., and Guengerich, F.P. (1997) Chem. Res. Toxicol. 10, 1133-1143. "Synthesis of Oligonucleotides Containing the Ethylene Dibromide-derived DNA Adducts S-[2-(N7-Guanyl)ethyl]-glutathione, S-[2-(N2-Guanyl)ethyl]glutathione,and S-[2-(O6-Guanyl)ethyl]glutathione at a Single Site."

Guengerich, F.P., and Johnson, W.W. (1997) Biochemistry 36, 14741-14750. "Kinetics of Ferric Cytochrome P450 Reduction by NADPH-Cytochrome P450 Reductase. Rapid Reduction in Absence of Substrate and Variation Among Cytochrome P450 Systems."

Furge, L.L., and Guengerich, F.P. (1998) Biochemistry 37, 3567-3574. "Pre-Steady-State Kinetics of Nucleotide Insertion Following 8-Oxo-7,8-dihydroguanine Base Pair Mismatches by Bacteriophage T7 DNA Polymerase exo-."

Kim, M-S., and Guengerich, F.P. (1998) Chem. Res. Toxicol. 11, 311-316. "Polymerase Blockage and Misincorporation of dNTPs Opposite the Ethylene Dibromide-derived DNA Adducts S-[2-(N7-Guanyl)ethyl]glutathione, S-[2-(N2-Guanyl)ethyl]glutathione, and S-[2-(O6-Guanyl)ethyl]-glutathione."

Langouet, S., Mican, A.N., Muller, M., Fink, S.P., Marnett, L.J., Muhle, S.A., and Guengerich, F.P. (1998) Biochemistry 37, 5184-5193. "Misincorporation of Nucleotides Opposite Three 5-Membered Exocyclic Ring Guanine Derivatives: 1,N2-Ethenoguanine, 5,6,7,9-Tetrahydro-9-oxoimidazo[1,2-a]purine, and 5,6,7,9-Tetrahydro-7-hydroxy-9-oxoimidazo[1,2-a]purine."

Hosea, N.A., and Guengerich, F.P. (1998) Arch. Biochem. Biophys. 353, 365-373. "Oxidation of Non-ionic Detergents by Cytochrome P450 Enzymes." 12879

Turesky, R.J., Constable, A., Richoz, J., Vargu, N., Markovic, J., Martin,M.V., and Guengerich, F.P. (1998) Chem. Res. Toxicol. 11, 925-936. "Differences in Activation of Heterocyclic Aromatic Amines by Rat and Human Liver Microsomes and by Rat and Human Cytochromes P450 1A2."

Shimada, T., Wunsch, R.M., Hanna, I.H., Sutter, T.R., Guengerich, F.P., and Gillam, E.M.J. (1998) Arch. Biochem. Biophys. 357, 111-120. "Recombinant Human Cytochrome P450 1B1 Expression in Escherichia coli." 12888 Shimada, T., Yamazaki, H., Foroozesh, M., Hopkins, N.E., Alworth, W.L., and Guengerich, F.P. (1998) Chem Res. Toxicol. 11, 1048-1056. "Selectivity of Polycyclic Inhibitors for Human Cytochromes P450 1A1, 1A2, and 1B1."

Einolf, H.F., Schnetz-Boutaud, N., and Guengerich, F.P. (1998) Biochemistry 37, 13300-13312. "Steady-state and Pre-Steady-state Kinetics of 8-Oxo-7,8-dihydroguanosine Triphosphate Incorporation and Extension by Replicative and Repair Polymerases."

Furge, L.L., and Guengerich, F.P. (1999, in press) Biochemistry "Explanation of Pre-Steady-State Kinetics and Decreased Burst Amplitude of HIV-1 Reverse Transcriptase at Sites of Modified DNA Bases with an Additional Non-Productive Enzyme-DNA-Nucleotide Complex."

For further information about Fred Guengerich and his research, please visit his professional website.

What does Dr. Guengerich say about his Olis DW-2?