Pump-Probe & flash photolysis are extensions of absorption spectroscopy. The absorbance at a particular wavelength or range of wavelengths of a sample is measured as a function of time after excitation by a flash of light. In a typical experiment, the light excitation is generated by a pulsed laser or LED. Absorbance, fluorescence and circular dichroism are useful tools for monitoring kinetic changes of chemical and biological systems. Flash photolysis measurements are initiated with an intense short light pump pulse from a laser, flash lamp, or LED. The excited electronic state caused by the pump pulse induces a physical or chemical change observed by absorbance or fluorescence. OLIS systems target changes in the microsecond to second range.
DB 620 UV/Vis [NIR] Spectrophotometer
Olis RSM 1000 UV/Vis[NIR] rapid-scanning spectrophotometer
Today, flash photolysis and pump-probe are extensively used by researchers to study:
- light-induced processes in organic molecules
- photosynthesis in plants
- light induced conformation changes in biological systems