Stopped Flow

What is Stopped Flow?

Absorbance, fluorescence, and circular dichroism are useful tools for monitoring kinetic changes of chemical and biological systems. However, changes often occur on the millisecond scale, too fast for simple mixing. A stopped-flow accessory is used on the spectrophotometer to achieve fast mixing.Reagents are injected from two drive syringes through a mixer into the observation cell, where the changes in absorbance, fluorescence, or circular dichroism are recorded. Reactions ranging from a few milliseconds to hundreds of seconds can be studied with the stopped-flow method.

Stopped Flow Applications

Typical research areas include reaction mechanisms, drug-binding processes, and determination of protein structure. Other applications include

  • Ligand binding
  • Coordination reactions
  • Chemical reactions
  • Electron transfer
  • Fluorescence resonance energy transfer (FRET)
  • Protein folding
  • Enzyme reactions
  • Protein-protein interactions
Models which support Stopped Flow
The DM 45 Series

The DM 45 Series

DSM 1000 Spectrophotometer

DSM 1000 Spectrophotometer

Olis RSM 1000 UV/Vis[NIR] Rapid-scanning Spectrophotometer

Olis RSM 1000 UV/Vis[NIR] rapid-scanning spectrophotometer