The Olis™ DSM 20 CD Spectrophotometer

far UV and visible [170-700 nm]

The model 20 uses the the new DeSa subtractive double grating monochromator for selecting and scanning the wavelengths. This tiny 7-sided monochromator uses two huge 40 x 45 mm gratings for outstanding light throughput, even down to the lowest wavelengths. These holographically blazed gratings can be for ultraviolet, UV/Vis, visible, Vis-NIR, or NIR. All associated light sources and detectors will be selected by Olis to be compatible with this blazed grating range. The spectral resolution is 0.5-5 nm, based on the slit width used; slits are interchangeable fixed slits of 0.5, 0.6, 1.24, and 2 mm wide, with other sizes available by special request. Like the Olis DSM 17, it can be enhanced to support scanning (or fixed wavelength) excitation and fixed wavelength emission; it is slightly superior to the model 17 in its excitation intensity performance.

February 2009: 'Two story' or 'skirted' Olis DSM 20 CD, allowing us to return to use of the UV enhanced Osram lamp. In addition to a (vertically-oriented lamp), this system includes (slit wheel assemblies) for ease in choosing appropriate slit widths and (electronics housed underneath the optics) rather than in a free-standing box.

The Olis DSM 20 CD with USA Stopped-Flow attached.

Gratings	Spectral Range	Wavelength Resolution	Applications	Detectors
The gratings are optics which determine the spectral range, wavelength resolution, and applications. Easily accessed and changed in the Olis DSM 20 spectrophotometer	The spectral range is fixed by the gratings. Gratings in the Olis Hummingbird monochromator can be changed with a minute’s effort.	The bandpass sets spectral resolution, which is determined by the gratings and slits used. Wavelength resolution for two slit widths are presented; slit widths optionally available are 0.280, 0.600, 0.760, 1.24, 1.56, 3.62 mm.	Secondary structure determination mandates first grating pair (usual range for a protein is 260-190 nm, or to 185, 180, or 175 nm if the sample preparation permits).	For Digital Subtractive CD, fast and sensitive detectors are used. PMTs are useful from UV to around 850 nm; InGaAs detectors with Olis fast response electronics are used from ~600-1650 nm
1200 lines/mm; blaze = 250 nm	170-700 nm	with 0.12 mm slit, 0.5 nm with 0.5 mm slit, 2 nm	Secondary protein structure determination; tertiary structure information	Photomultiplier tubes (PMTs)
1200 lines; 350 nm	250-800 nm	with 0.12 mm slit, 0.5 nm, with 0.5 mm slit, 2 nm	Tertiary protein structure information	PMTs
800 lines; 600 nm	350-1200 nm	with 0.12 mm slit, 0.7 nm with 0.5 mm slit, 3 nm	Nanostructures, Polymers, Metal ion chelation	PMTs and InGaAs
600 lines; 750 nm	400-1650 nm	with 0.12 mm slit, 1 nm with 0.5 mm slit, 4 nm	Nanostructures, Polymers, Metal ion chelation	PMTs and InGaAs

See how the DSM 20 compares to our other CD models.

View client data collected with the DSM 10 CD (the predecessor of the DSM 20).

Download our PDF flyer for the DSM 20 CD.

Download a PDF brochure on the new Hummingbird Monochromator

View the LC 600™ 6-position cell changer from Quantum Northwest.

Take a closer look at the DSM CD sample compartment and associated hardware.