Four Syringe Automated Enzyme Assay Device

• Use for automated preparation of enzyme, substrate, inhibitor, and buffer mixtures.

• Standard syringe volumes are 2.5 mL for inhibitor, substrate, and enzyme, and 10 mL for the buffer, which will be used for flushing the cuvette between measurements, too.

• Volumes from each syringe can be 0.0 or 0.0001-100% and are specified in
  user-created ASCII script.

• Interfaces directly with all Olis spectrophotometers and can be used freestanding.

In this model, volumes from syringes 1, 2, and 3 are mixed in the mixing valve and volume of syringe 4 is mixed further down the line, nearer the measuring cuvette (Fig. 1, below). Thus, the appropriate mixture of inhibitor, substrate and buffer is prepared and then, right before the mixture shoots into the measuring cuvette, the reactant—the enzyme—is added, too. Then, after the scan, instead of the mixture being withdrawn into any of the syringes, it is forced out of the system by a flush of buffer. Thus, the measuring cuvette is thoroughly rinsed with buffer between each run. The syringes will be (for example) 2.5 mL for inhibitor, substrate, and enzyme, and 10 mL for the buffer.

There is a great deal of flexibility in our designs, both explicitly and implicitly. Volumes added can be anything from 0.0 or 0.0001 of the syringe volume to 100% of it. Mixing can be done any number of times. Changing syringes from one size to another is a 5 minute operation. And any number of scans can be made of each dilution.

A Peltier temperature-controlled enzyme assay device is also available.
Click here for more information.

We offer a Peltier temperature-controlled 4 position cell holder.
Click here for more information.

The Olis Short Pathlength Titrator