Olis DSM CD with Stopped-Flow

Combining circular dichroism (CD) with Stopped-flow speed mixing means detecting milliabsorbance signals on a millisecond time scale. Rendering this difficult measurement more practical is the Olis CD stopped-flow spectrophotometer.

Fixed wavelength detection, recommended for all the more difficult measurements including UV readings, is done by sampling 1,000 times per second. With this millisecond detection rate, one can obtain excellent kinetic traces and calculate rate constants. Rapid-scanning detection, suitable for all of the easier measurements, including visible readings, can be done by sampling at rates to 62 CD scans per second (please see PDF documentation for more information). With this millisecond spectral detection rate, one can obtain kinetics at hundreds of wavelengths, supporting not only rate constant calculation but also spectral reconstruction.

This Olis DSM CD module and Olis CDSF can be used with the Olis RSM 1000 for fixed wavelength, conventional speed scanning, and rapid-scanning work; and the Olis DSM CD and CDSF can be used with other Olis monochromators for fixed wavelength and conventional speed scanning. Spectral ranges and prices dependent upon selected monochromator.

Pictured in the photo below is a view of the stopped-flow not attached to the CD module and a close up of the observation cell and the insulated tubing for the circulating heating/cooling fluids.