The Olis Short Pathlength Titrator

The Olis Automatic Titrator supports use of all dimension cuvettes, rendering it as useful in UV CD of proteins as it is in absorbance and fluorescence work. Stirring within the cuvette is not required, so cuvette pathlengths down to 0.1 mm are as suitable as 1 cm² or wider cuvettes. Mixing is accomplished by computerized infusion and withdrawal cycles from the syringes to the measuring cell. The sample is perturbed to the minimum level, so sudsing issues are completely avoided. Anaerobicity can be maintained throughout the experiment. And the sample is in the measuring beam only during the measurement, so photolysis is held to a minimum. Mixing is complete. Delay times to wait for equilibrium are user-selectable. Volumes are based on cuvette pathlength. Dilution ratio of sample and titrant are arbitrary. There are no 'overflow' issues; sample is 'mixed and stored' in an external syringe until time of optical measurement; and volume to be measured is precisely what is needed to fill cuvette (any 'overflow' remains in mixing/storage syringe). In-line pH microelectrode can be positioned within flow line from storage syringe to sample cell. The sample is in the light path only when measurements are made.

Users of the Olis' U.S.A. Stopped-Flow will recognize the trademark user friendly knobs. (No piddly little switches here!) The accessory is also mounted on a thick metal sheet, continuing Olis tradition of creating a sturdy, lifelong product.

Motors and electrical components are neatly housed in this "box" which is then connected to a main control box.

Titrator number 1 (pictured above) was purchased by Dr. Jeffrey Moore of the University of Illinois.

Titrator Operation Details

Description and screenshot of manual control panel in SpectralWorks.

Sample Data: Titrator Dependent CD Scans

4-syringe peltier-controlled enzyme assay device