Paul's Practical Guide - Table 1
Cutoff Wavelengths For Common Solvents and Buffers
| Solvent System |
Cut off (nm) |
Water
Trifluoroethanol
Hexafluoroisopropanol
Acetonitrile
Methanol
Ethanol
2-Propanol
Cyclohexane
Dimethylsulfoxide
Dioxane
|
<185
<185
<185
185
195
196
196
<185
251
232
|
(NH4)2SO4 0.15 M
NaCl 0.15 M
NaClO4 0.15 M
NaNO3 0.15 M
|
191
196
<185
245
|
Phosphate 100 mM
Tris 100 mM
Pipes 100 mM
Mes 100 mM
|
<185
195
215
205
|
GdnHCl 4 M
Urea 4 M
|
210
210
|
(For one mm pathe lenth.)
|
