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The RSM as Spectrofluorimeter
Evaluating the Performance of the Olis RSM 1000 in its Fluorescence Modes
After 45 absorbance model RSM systems were delivered, we responded to encouragement to design a model fully optimized for fluorescence. (Pictured here is an Olis RSM 1000F fitted with a 450 watt lamp and U.S.A. Stopped-Flow). Emission scan rates to 1,000 scans/second are achievable on the finalized system that uses a 450 watt xenon arc lamp as the exciting source, a single grating monochromator as the exciting light wavelength selector, and the RSM with a mid-plane PMT.
Issues with the bright 450 watt xenon arc lamp - heat and stray light - were addressed by the following new components:
Beam-Cooling Accessory
Positioned between the lamp housing and the monochromator that removes the IR (the heat) from the output beam. "Sister Monochromator"
Featuring a 40x45 mm concave holographic grating driven by a small servo motor; the unit has conventional entrance and exit slits and a place for a second-order filter; provides high light throughput, affords the equivalent wavelength range of scores of filters, and can be adjusted using a simple keystroke command to the software to select excitation wavelengths. Closed-Cycle Cooling Box
Cools both the lamp housing and the output beam without requiring an external source of water. Middle-Plane PMT
Optimizes photosensitivity; attached to a trap-door lid, this high-gain, side-window PMT intercepts the emission beam at the middle plane of the RSM, maximizing detection of weak luminescence.
These innovative additions transform the world's only rapid-scanning spectrophotometer into the world's only rapid-scanning spectrofluorometer. Providing extraordinary sensitivity, resolution, and speed in monitoring rapidly changing samples, both configurations are available in one benchtop workstation!
Click here for a pictorial comparison of the RSM in five popular configurations.
 View details on our newest fluorometer, the DM 45.
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