| Gratings |
Spectral Range |
Wavelength Resolution |
Applications |
Detectors |
| The gratings determine the spectral range wavelength resolution. They are easily accessible within the Hummingbird monochromator and are exhanged without tools. Calibration is automatic. |
The spectral range is fixed by the gratings. Gratings in the Olis Hummingbird monochromator can be changed with a minute's effort. |
The bandpass sets spectral resolution, which is determined by the gratings and slits used.
Wavelength resolution for two slit widths are presented; slit widths optionally available are 0.280, 0.600, 0.760, 1.24, 1.56, 3.62 mm. |
Secondary structure determination (CD) mandates first grating pair (usual range for a protein is 260-190 nm). |
Fast and sensitive PMTs are useful from UV to around 850 nm; InGaAs detectors with Olis fast response electronics are useful from ~600-1650 nm (standard) or ~800-2600 nm (extended range). Photon counting detectors are useful for high sensitivity emission. |
1200 lines/mm;
blazed at 250 nm |
165-720 nm |
with 0.12 mm slit, 0.5 nm
with 0.5 mm slit, 2 nm |
Secondary protein structure determination; tertiary structure information |
Photomultiplier tubes (PMTs) |
| 1200 lines; 350 nm |
250-800 nm |
with 0.12 mm slit, 0.5 nm,
with 0.5 mm slit, 2 nm |
CD: Tertiary protein structure information |
PMTs or Photon counting detectors |
| 800 lines; 600 nm |
350-1200 nm |
with 0.12 mm slit, 0.7 nm
with 0.5 mm slit, 3 nm |
Absorbance, Fluorescence:Nanostructures, Polymers, Metal ion chelation |
PMTs and InGaAs |
| 600 lines; 750 nm |
400-1650 nm |
with 0.12 mm slit, 1 nm
with 0.5 mm slit, 4 nm |
Absorbance, Fluorescence:Nanostructures, Polymers, Metal ion chelation |
PMTs and InGaAs |
| 1200 lines; 1000 nm |
500-2500 nm |
with 0.12 mm slit, 0.5 nm
with 0.5 mm slit, 2 nm |
Absorbance, Fluorescence:Nanostructures, Polymers, Metal ion chelation |
PMTs and InGaAs |
